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Volume 11, Number 7—July 2005
Dispatch

SARS Coronavirus Detection Methods

Susanna K.P. Lau*, Xiao-Yan Che†, Kwok-Yung Yuen*, Beatrice H.L. Wong*, Vincent C.C. Cheng*, Gibson K.S. Woo*, Ivan F.N. Hung‡, Rosana W.S. Poon*, Kwok-Hung Chan*, J.S. Malik Peiris*, and Kwok-Yung Yuen*Comments to Author 
Author affiliations: *University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China; †First Military Medical University, Guangzhou, People's Republic of China; ‡Queen Mary Hospital, Hong Kong Special Administrative Region, People's Republic of China

Main Article

Table 2

Detection of SARS-CoV by qRT-PCR and ELISA in clinical specimens of patients with SARS*

RNA concentration (copies/mL) Fecal specimens
Urine specimens
No. specimens No. positive by polyclonal antibody–based ELISA No. positive by monoclonal antibody–based ELISA No. specimens No. positive by polyclonal antibody–based ELISA No. positive by monoclonal antibody–based ELISA
<3 × 102 8 3 0 100 2 6
3 × 102–<104 5 3 3 16 1 1
104–<106 3 0 0 10 1 1
106–<108 9 5 0 7 2 3
108–<1010 13 8 10 0 0 0
≥1010 2 1 1 0 0 0
Total 40 20 14 133 6 11

*SARS-CoV, severe acute respiratory syndrome coronavirus; qRT-PCR, quantitative reverse transcription–polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay.

Main Article

Page created: April 23, 2012
Page updated: April 23, 2012
Page reviewed: April 23, 2012
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