Volume 11, Number 7—July 2005
SARS Vaccine Development
|Characteristics||SARS-CoV–like virus||Early human SARS-CoV||Late human SARS-CoV|
|Representative strains||SZ3, SZ16||GD03T0013||Tor2, Urbani, BJ01, GZ02|
|Source||Palm civets||SARS patients during 2003–2004 epidemic||SARS patients during 2002–2003 outbreak|
|29-nucleotide deletion||No||No||Yes (some have a 415– nucleotide deletion)|
|Properties of spike protein|
|Rate of nonsynonymous mutation||High||High||Low|
|Binding affinity to ACE2||Low||Low||High|
- Peiris JS, Guan Y, Yuen KY. Severe acute respiratory syndrome. Nat Med. 2004;10:S88–97.
- Snijder EJ, Bredenbeek PJ, Dobbe JC, Thiel V, Ziebuhr J, Poon LLM, Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage. J Mol Biol. 2003;331:991–1004.
- Guan Y, Zheng BJ, He YQ, Liu XL, Zhuang ZX, Cheung CL, Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China. Science. 2003;302:276–8.
- Chinese SARS Molecular Epidemiology Consortium. Molecular evolution of the SARS coronavirus during the course of the SARS epidemic in China. Science. 2004;303:1666–9.
- Guan Y, Peiris JS, Zheng B, Poon LL, Chan KH, Zeng FY, Molecular epidemiology of the novel coronavirus that causes severe acute respiratory syndrome. Lancet. 2004;363:99–104.
- Yang ZY, Werner HC, Kong WP, Leung K, Traggiai E, Lanzavecchia A, Evasion of antibody neutralization in emerging severe acute respiratory syndrome coronaviruses. Proc Natl Acad Sci U S A. 2005;102:797–801.
- Seto WH, Tsang D, Yung RWH, Ching TY, Ng TK, Ho M, Effectiveness of precautions against droplets and contact in prevention of nosocomial transmission of severe acute respiratory syndrome (SARS). Lancet. 2003;361:1519–20.
- Xiong S, Wang YF, Zhang MY, Liu XJ, Zhang CH, Liu SS, Immunogenicity of SARS inactivated vaccine in BALB/c mice. Immunol Lett. 2004;95:139–43.
- He Y, Zhou Y, Siddiqui P, Jiang S. Inactivated SARS-CoV vaccine elicits high titers of spike protein-specific antibodies that block receptor binding and virus entry. Biochem Biophys Res Commun. 2004;325:445–52.
- Chou TH, Wang S, Sakhatskyy PV, Mboudoudjeck I, Lawrence JM, Huang S, Epitope mapping and biological function analysis of antibodies produced by immunization of mice with an inactivated Chinese isolate of severe acute respiratory syndrome–associated coronavirus (SARS-CoV). Virology. 2005;334:134–43.
- Qu D, Zheng B, Yao X, Guan Y, Yuan ZH, Zhong NS, Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice. Vaccine. 2005;23:924–31.
- Marshall E, Enserink M. Medicine. Caution urged on SARS vaccines. Science. 2004;303:944–6.
- Wang D, Lu J. Glycan arrays lead to the discovery of autoimmunogenic activity of SARS-CoV. Physiol Genomics. 2004;18:245–8.
- Holmes KV. SARS-associated coronavirus. N Engl J Med. 2003;348:1948–51.
- Li WH, Moore MJ, Vasilieva NY, Sui JH, Wong SK, Berne AM, Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature. 2003;426:450–4.
- Prabakaran P, Xiao X, Dimitrov DS. A model of the ACE2 structure and function as a SARS-CoV receptor. Biochem Biophys Res Commun. 2004;314:235–41.
- Wong SK, Li W, Moore MJ, Choe H, Farzan M. A 193-amino-acid fragment of the SARS coronavirus S protein efficiently binds angiotensin-converting enzyme 2. J Biol Chem. 2003;279:3197–201.
- Xiao X, Chakraborti S, Dimitrov AS, Gramatikoff K, Dimitrov DS. The SARS-CoV S glycoprotein: expression and functional characterization. Biochem Biophys Res Commun. 2003;312:1159–64.
- Dimitrov DS. The secret life of ACE2 as a receptor for the SARS virus. Cell. 2003;115:652–3.
- Yang ZY, Huang Y, Ganesh L, Leung K, Kong WP, Schwartz O, pH-dependent entry of severe acute respiratory syndrome coronavirus is mediated by the spike glycoprotein and enhanced by dendritic cell transfer through DC-SIGN. J Virol. 2004;78:5642–50.
- Jeffers SA, Tusell SM, Gillim-Ross L, Hemmila EM, Achenbach JE, Babcock GJ, CD209L (L-SIGN) is a receptor for severe acute respiratory syndrome coronavirus. Proc Natl Acad Sci U S A. 2004;101:15748–53.
- Liu S, Xiao G, Chen Y, He Y, Niu J, Escalante C, Interaction between the heptad repeat 1 and 2 regions in spike protein of SARS-associated coronavirus: implication for virus fusogenic mechanism and identification of fusion inhibitors. Lancet. 2004;363:938–47.
- Tripet B, Howard MW, Jobling M, Holmes RK, Holmes KV, Hodges RS. Structural characterization of the SARS-coronavirus spike S fusion protein core. J Biol Chem. 2004;279:20836–49.
- Xu Y, Lou Z, Liu Y, Pang H, Tien P, Gao GF, Crystal structure of SARS-CoV spike protein fusion core. J Biol Chem. 2004;279:49414–9.
- Yang ZY, Kong WP, Huang Y, Roberts A, Murphy BR, Subbarao K, A DNA vaccine induces SARS coronavirus neutralization and protective immunity in mice. Nature. 2004;428:561–4.
- Wang S, Chou TH, Sakhatskyy PV, Huang S, Lawrence JM, Cao H, Identification of two neutralizing regions on the severe acute respiratory syndrome coronavirus spike glycoprotein produced from the mammalian expression system. J Virol. 2005;79:1906–10.
- Keng CT, Zhang A, Shen S, Lip KM, Fielding BC, Tan TH, Amino acids 1055 to 1192 in the S2 region of severe acute respiratory syndrome coronavirus s protein induce neutralizing antibodies: implications for the development of vaccines and antiviral agents. J Virol. 2005;79:3289–96.
- Zhong X, Yang H, Guo ZF, Sin WY, Chen W, Xu J, B-cell responses in patients who have recovered from severe acute respiratory syndrome target a dominant site in the S2 domain of the surface spike glycoprotein. J Virol. 2005;79:3401–8.
- Bisht H, Roberts A, Vogel L, Bukreyev A, Collins PL, Murphy BR, Severe acute respiratory syndrome coronavirus spike protein expressed by attenuated vaccinia virus protectively immunizes mice. Proc Natl Acad Sci U S A. 2004;101:6641–6.
- Bukreyev A, Lamirande EW, Buchholz UJ, Vogel LN, Elkins WR, St Claire M, Mucosal immunisation of African green monkeys (Cercopithecus aethiops) with an attenuated parainfluenza virus expressing the SARS coronavirus spike protein for the prevention of SARS. Lancet. 2004;363:2122–7.
- He Y, Zhou Y, Wu H, Luo B, Chen J, Li W, Identification of immunodominant sites on the spike protein of severe acute respiratory syndrome (SARS) coronavirus: implication for developing SARS diagnostics and vaccines. J Immunol. 2004;173:4050–7.
- Olsen CW, Corapi WV, Jacobson RH, Simkins RA, Saif LJ, Scott FW. Identification of antigenic sites mediating antibody-dependent enhancement of feline infectious peritonitis virus infectivity. J Gen Virol. 1993;74:745–9.
- Jiang S, Lin K, Neurath AR. Enhancement of human immunodeficiency virus type-1 (HIV-1) infection by antisera to peptides from the envelope glycoproteins gp120/gp41. J Exp Med. 1991;174:1557–63.
- Weingartl H, Czub M, Czub S, Neufeld J, Marszal P, Gren J, Immunization with modified vaccinia virus Ankara-based recombinant vaccine against severe acute respiratory syndrome is associated with enhanced hepatitis in ferrets. J Virol. 2004;78:12672–6.
- He Y, Zhu Q, Liu S, Zhou Y, Yang B, Li J, Identification of a critical neutralization determinant of severe acute respiratory syndrome (SARS)-associated coronavirus: importance for designing SARS vaccines. Virology. 2005;334:74–82.
- Chen Z, Zhang L, Qin C, Ba L, Yi CE, Zhang F, Recombinant modified vaccinia virus Ankara expressing the spike glycoprotein of severe acute respiratory syndrome coronavirus induces protective neutralizing antibodies primarily targeting the receptor binding region. J Virol. 2005;79:2678–88.
- He Y, Zhou Y, Liu S, Kou Z, Li W, Farzan M, Receptor-binding domain of SARS-CoV spike protein induces highly potent neutralizing antibodies: implication for developing subunit vaccine. Biochem Biophys Res Commun. 2004;324:773–81.
- He Y, Lu H, Siddiqui P, Zhou Y, Jiang S. Receptor-binding domain of SARS coronavirus spike protein contains multiple conformation-dependent epitopes that induce highly potent neutralizing antibodies. J Immunol. 2005;174:4908–15.
- van den Brink EN, Ter Meulen J, Cox F, Jongeneelen MA, Thijsse A, Throsby M, Molecular and biological characterization of human monoclonal antibodies binding to the spike and nucleocapsid proteins of severe acute respiratory syndrome coronavirus. J Virol. 2005;79:1635–44.
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