Nareg Roubinian*, Beth D. Kirkpatrick*, Freyja Lynn†, Jonathan Zenilman‡, and Margaret Bash†
Figure. . Pre- and posttransplantation serum antibodies as measured by enzyme-linked immunosorbent assay (ELISA). A) Immunoglobulin (Ig) G antibodies to Neisseria meningitidis serogroup C capsular polysaccharide (CPS) determined as described by Arakere and Frasch (7) with minor modifications. Samples were run in duplicate at 8 serial dilutions, and antibody concentrations were calculated relative to the standard reference serum lot CDC 1992 (courtesy of G. Carlone, Centers for Disease Control and Prevention, Atlanta, GA). B) IgM antibodies to N. meningitidis lipooligosaccharide (LOS) immunotypes (L2, L3, L7, L9). ELISA to detect antibodies to LOS immunotypes L2, L3, L7, or L9 was performed as described (8), with minor modifications, by using goat antihuman IgG (γ-specific, Kirkegaard & Perry, Gaithersburg, MD, USA) or goat antihuman IgM (μ-specific, Sigma, St. Louis, MO, USA) conjugated to alkaline phosphatase. Samples were run in duplicate at 4 serial dilutions. OD, optical density.