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Volume 11, Number 8—August 2005
Letter

Neisseria meningitidis Endotoxin and Capsule Transmission by Transplantation

Nareg Roubinian*, Beth D. Kirkpatrick*, Freyja Lynn†, Jonathan Zenilman‡, and Margaret Bash†Comments to Author 
Author affiliations: *University of Vermont College of Medicine, Burlington, Vermont, USA; †US Food and Drug Administration, Rockville, Maryland, USA; ‡Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

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Figure

Pre- and posttransplantation serum antibodies as measured by enzyme-linked immunosorbent assay (ELISA). A) Immunoglobulin (Ig) G antibodies to Neisseria meningitidis serogroup C capsular polysaccharide (CPS) determined as described by Arakere and Frasch (7) with minor modifications. Samples were run in duplicate at 8 serial dilutions, and antibody concentrations were calculated relative to the standard reference serum lot CDC 1992 (courtesy of G. Carlone, Centers for Disease Control and Preventi

Figure. . Pre- and posttransplantation serum antibodies as measured by enzyme-linked immunosorbent assay (ELISA). A) Immunoglobulin (Ig) G antibodies to Neisseria meningitidis serogroup C capsular polysaccharide (CPS) determined as described by Arakere and Frasch (7) with minor modifications. Samples were run in duplicate at 8 serial dilutions, and antibody concentrations were calculated relative to the standard reference serum lot CDC 1992 (courtesy of G. Carlone, Centers for Disease Control and Prevention, Atlanta, GA). B) IgM antibodies to N. meningitidis lipooligosaccharide (LOS) immunotypes (L2, L3, L7, L9). ELISA to detect antibodies to LOS immunotypes L2, L3, L7, or L9 was performed as described (8), with minor modifications, by using goat antihuman IgG (γ-specific, Kirkegaard & Perry, Gaithersburg, MD, USA) or goat antihuman IgM (μ-specific, Sigma, St. Louis, MO, USA) conjugated to alkaline phosphatase. Samples were run in duplicate at 4 serial dilutions. OD, optical density.

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References
  1. Loinaz  C, Moreno Gonzalez  E. Marginal donors in liver transplantation. Hepatogastroenterology. 2000;47:25663.PubMed
  2. Lopez-Navidad  A, Domingo  P, Cabellero  F, Gonzalez  C, Santiago  C. Successful transplantation of organs retrieved from donors with bacterial meningitis. Transplantation. 1997;64:3658. DOIPubMed
  3. Satoi  S, Bramhall  SR, Solomon  M, Hastings  M, Mayer  AD, De Goyet  JV, The use of liver grafts from donors with bacterial meningitis. Transplantation. 2001;72:110813. DOIPubMed
  4. Nery  JR, Weppler  D, Ketchum  P, Olson  L, Fragulidis  GP, Khan  MF, Donor infection and primary nonfunction in liver transplantation. Transplant Proc. 1997;29:4813. DOIPubMed
  5. Yokoyama  I, Todo  S, Miyata  T, Selby  R, Tzakis  AG, Starzi  TE. Endotoxemia and human liver transplantation. Transplant Proc. 1989;21:383341.PubMed
  6. Campbell  JD, Edelman  R, King  JC Jr, Papa  T, Ryall  R, Rennels  MB. Safety, reactogenicity, and immunogenicity of a tetravalent meningococcal polysaccharide-diphtheria toxoid conjugate vaccine given to healthy adults. J Infect Dis. 2002;186:184851. DOIPubMed
  7. Arakere  G, Frasch  CE. Specificity of antibodies to O-acetyl-positive and O-acetyl-negative group C meningococcal polysaccharides in sera from vaccinees and carriers. Infect Immun. 1991;59:434956.PubMed
  8. Tsai  CM, Civin  CI. Eight lipooligoshaccharides of Neisseria meningitidis react with a monoclonal antibody which binds lacto-N-neotetraose (GalB1-4GlcNAcB1-3GalB1-4Glc). Infect Immun. 1991;59:36049.PubMed
  9. Brandtaeg  P, Kierulf  P, Gaustad  P, Skulberg  A, Brunn  J, Halvorse  S, Plasma endotxoin as a predictor of multiple organ failure and death in systemic meningococcal disease. J Infect Dis. 1989;159:195204. DOIPubMed
  10. Zipfel  A, Schenk  M, You  M-S, Lauchart  W, Bode  C, Viebahn  R. Endotoxemia in organ donors: graft function following liver transplantation. Transpl Int. 2000;13(Suppl 1):S286–7.

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