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Volume 12, Number 10—October 2006
Dispatch

Chimpanzee Adenovirus Antibodies in Humans, Sub-Saharan Africa

Zhiquan Xiang*, Yan Li*, Ann Cun*, Wei Yang†, Susan Ellenberg†, William M. Switzer‡, Marcia L. Kalish‡, and Hildegund C.J. Ertl*Comments to Author 
Author affiliations: *The Wistar Institute, Philadelphia, Pennsylvania, USA; †University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA; ‡Centers for Disease Control and Prevention, Atlanta, Georgia, USA

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Table 1

Sera with neutralizing activity to different human and chimpanzee adenoviruses

Origin % positive samples (p values)*†
AdHu5 AdC68 AdC6 AdC1
Human controls, United States (n = 50) 34.0 2.0 4.0 2.0
Human zoo workers, United States (n = 50) 28.0 0 0 0
Humans, Thailand (n = 200) 76.5 1.5 3.0 4.0
Humans, Cameroon (n = 405) 55.8 1.7 (0.6764) 7.9 (0.0045) 5.4 (0.1248)
Humans, Côte d'Ivoire (n = 169) 95.8 9.5 (0.0003) 10.7 (0.0008) 3.0 (0.9796)
Humans, Nigeria (n = 182) 89.0 4.9 (0.0267) 18.7 (<0.0001) 9.3 (0.0045)
Chimpanzees, United States (n = 50) 44.0 86.0 (<0.0001) 92.0 (<0.0001) 46.0 (<0.0001)

*p values show statistical difference between percentages of sera positive for neutralizing antibodies to human and chimpanzee adenoviruses. Reactivity of human sera from Cameroon, Côte d'Ivoire, and Nigeria and of chimpanzee sera to the 3 chimpanzee-derived adenoviruses were compared with human sera from the United States (n = 100) and Thailand (n = 200); the last 2 were combined because these countries do not offer natural chimpanzee habitats (similarity of USA and Thailand data for these adenoviruses was statistically confirmed). A logistic regression model was fitted to compare the percentages of samples positive for neutralizing antibodies between different groups. A p value <0.05 was considered statistically significant. All analyses were performed by using SAS version 9.1 logistic procedure (9).
†Virus tested for neutralization with a previously described neutralization assay (10). Samples that neutralized virus at dilutions >1:20 were scored as positive.

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References
  1. Xiang  ZQ, Yang  Y, Wilson  JM, Ertl  HC. A replication-defective human adenovirus recombinant serves as a highly efficacious vaccine carrier. Virology. 1996;219:2207. DOIPubMed
  2. Shiver  JW, Fu  TM, Chen  L, Casimiro  DR, Davies  ME, Evans  RK, Replication-incompetent adenoviral vaccine vector elicits effective anti-immunodeficiency-virus immunity. Nature. 2002;415:3315. DOIPubMed
  3. Vinner  L, Wee  EG, Patel  S, Corbet  S, Gao  GP, Nielsen  C, Immunogenicity in Mamu-A*01 rhesus macaques of a CCR5-tropic human immunodeficiency virus type 1 envelope from the primary isolate (Bx08) after synthetic DNA prime and recombinant adenovirus 5 boost. J Gen Virol. 2003;84:20313. DOIPubMed
  4. Fitzgerald  JC, Gao  GP, Reyes-Sandoval  A, Pavlakis  GN, Xiang  ZQ, Wlazlo  AP, A simian replication-defective adenoviral recombinant vaccine to HIV-1 gag. J Immunol. 2003;170:141622.PubMed
  5. Casimiro  DR, Chen  L, Fu  TM, Evans  RK, Caulfield  MJ, Davies  ME, Comparative immunogenicity in rhesus monkeys of DNA plasmid, recombinant vaccinia virus, and replication-defective adenovirus vectors expressing a human immunodeficiency virus type 1 gag gene. J Virol. 2003;77:630513. DOIPubMed
  6. Farina  SF, Gao  GP, Xiang  ZQ, Rux  JJ, Burnett  RM, Alvira  MR, Replication-defective vector based on a chimpanzee adenovirus. J Virol. 2001;75:1160313. DOIPubMed
  7. Shiver  JW. Gene therapy: chimpanzee-origin adenovirus vectors as vaccine. Banff, Alberta, Canada: Keystone Symposia; 2003.
  8. Basnight  M Jr, Rogers  NG, Gibbs  CJ Jr, Gajdusek  DC. Characterization of four new adenovirus serotypes isolated from chimpanzee tissue explants. Am J Epidemiol. 1971;94:16671.PubMed
  9. The LOGISTIC procedure. SAS version 9.1.3. Cary (NC): SAS Institute, Inc.; 2005.
  10. Reyes-Sandoval  A, Fitzgerald  JC, Grant  R, Roy  S, Xiang  ZQ, Li  Y, Human immunodeficiency virus type 1-specific immune responses in primates upon sequential immunization with adenoviral vaccine carriers of human and simian serotypes. J Virol. 2004;78:73929. DOIPubMed
  11. Tutin  CE. Ecology and social organization of African tropical forest primates: aid in understanding retrovirus transmission. Bull Soc Pathol Exot. 2000;93:15761.PubMed
  12. Hahn  BH, Shaw  GM, De Cock  KM, Sharp  PM. AIDS as a zoonosis: scientific and public health implications. Science. 2000;287:60714. DOIPubMed
  13. Switzer  WM, Bhullar  V, Shanmugam  V, Cong  M, Parekh  B, Lerche  NW, Frequent simian foamy virus infection in persons occupationally exposed to nonhuman primates. J Virol. 2004;78:27809. DOIPubMed
  14. Calattini  S, Maudere  P, Tortevoye  P, Froment  A, Saib  A, Gessain  A. Interspecies transmission of simian foamy virus from chimpanzees and gorillas to Bantus and Pygmy hunters in southern Cameroon [abstract]. Würzburg, Germany: Fifth International Foamy Virus Conference. 2004 Jul 9–1.
  15. Kostense  S, Koudstaal  W, Sprangers  M, Weverling  GJ, Penders  G, Helmus  N, Adenovirus types 5 and 35 seroprevalence in AIDS risk groups supports type 35 as a vaccine vector. AIDS. 2004;18:12136. DOIPubMed

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