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Volume 12, Number 10—October 2006
Dispatch

Nematode Symbiont for Photorhabdus asymbiotica

John Gerrard*Comments to Author , Susan Joyce†, David Clarke†, Richard ffrench-Constant‡, Graeme Nimmo§, David F.M. Looke¶, Edward Feil†, Lucy Pearce†, and Nick Waterfield†
Author affiliations: *Gold Coast Hospital, Southport, Queensland, Australia; †University of Bath, Bath, United Kingdom; ‡University of Exeter in Cornwall, Falmouth, United Kingdom; §Queensland Health Pathology Service, Brisbane, Queensland, Australia; ¶Princess Alexandra Hospital, Brisbane, Queensland, Australia

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Figure 2

Phylogenetic tree of concatenated sequences of fragments of the glnA gene (474 bp) and the gyrB gene (576 bp) in 52 Photorhabdus isolates representing known diversity across the genus. The tree was constructed with the neighbor-joining algorithm and the K2-P method of distance estimation as implemented in MEGA version 3.0 (12). A total of 1,000 bootstrap replicates were performed, and the percentage of bootstrap trees supporting each node are given. The Kingscliff isolate (arrow) clusters with P

Figure 2. Phylogenetic tree of concatenated sequences of fragments of the glnA gene (474 bp) and the gyrB gene (576 bp) in 52 Photorhabdus isolates representing known diversity across the genus. The tree was constructed with the neighbor-joining algorithm and the K2-P method of distance estimation as implemented in MEGA version 3.0 (12). A total of 1,000 bootstrap replicates were performed, and the percentage of bootstrap trees supporting each node are given. The Kingscliff isolate (arrow) clusters with P. asymbiotica isolates from Australia, both in the concatenated tree (bootstrap score = 100%) and in individual gene trees (not shown). The scale bar shows percentage relatedness.

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