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Volume 12, Number 11—November 2006
Research

Clostridium difficile PCR Ribotypes in Calves, Canada

Alexander Rodriguez-Palacios*Comments to Author , Henry R. Stämpfli*, Todd Duffield*, Andrew S. Peregrine*, Lise A. Trotz-Williams*, Luis G. Arroyo*, Jon S. Brazier†, and J. Scott Weese*
Author affiliations: *University of Guelph, Guelph, Ontario, Canada; †University Hospital of Wales, Cardiff, United Kingdom3

Main Article

Table 2

Clostridium difficile PCR ribotypes and toxin genes of 31 isolates obtained from dairy calves in southern Ontario, Canada, 2004

Toxigenic classification† Calf PCR ribotypes* Subtotal, no. (%)
A-11 B-89 C-129 D-189 E-257 F-12 H-75 I-157
A–B, cdtB 1 1 (3.2)
A–B, cdtB+ 2 2 (6.5)
A+B+, cdtB 2 4 1 1 8 (25.8)
A+B+, cdtB+ 7 4 11 (35.5)
A–B+, cdtB 8 8 (25.8)
A–B+, cdtB+ 1 1 (3.2)
Subtotal, no. (%) 7 (22.6) 9 (29) 2 (6.5) 4 (12.9) 4 (12.9) 3 (9.7) 1 (3.2) 1 (3.2) 31 (100)
Type of tcdC deletion‡, (no.) A (7) None (9) None (2) B (4) None (4) A (3) None (1) None (1)
Human PCR ribotypes§ 078 017 077 027 014 033 078 NS¶
*By PCR typing method of Bidet et al. (22). †A, toxic gene tcdA; B, tcdB; – absence or presence of the categorized gene; cdtB, gene that codifies CDTb, the binding segment of the binary toxin. ‡None, tcdC with no deletions (≈345 bp); A, type A deletion of ≈39 bp; B, nondifferentiated type B or C deletion of ≈18 bp (11). §Selected isolates processed at the Anaerobe Reference Laboratory, University Hospital of Wales, Cardiff, UK (23). ¶NS, not submitted for analysis.

Main Article

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Page updated: October 14, 2011
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