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Volume 12, Number 2—February 2006
Dispatch

Evaluation of a Direct, Rapid Immunohistochemical Test for Rabies Diagnosis

Tiziana Lembo*, Michael Niezgoda†, Andrés Velasco-Villa†, Sarah Cleaveland*, Eblate Ernest‡, and Charles E. Rupprecht†
Author affiliations: *University of Edinburgh, Midlothian, United Kingdom; †Centers for Disease Control and Prevention, Atlanta, Georgia, USA; ‡Tanzania Wildlife Research Institute, Arusha, Tanzania

Main Article

Figure 1

Touch impression of a rabies-positive Tanzanian domestic dog brain preserved in 50% glycerol saline solution for 15 months before testing by direct rapid immunohistochemical test (dRIT) and retested by direct fluorescent-antibody assay (DFA) after 5 months. A) Brain stained by dRIT: rabies virus antigen appears as magenta inclusions (arrowheads) against the blue neuronal hematoxylin counterstain. Magnification, ×630. B) Immunofluorescent apple-green viral inclusions in the same brain processed b

Figure 1. Touch impression of a rabies-positive Tanzanian domestic dog brain preserved in 50% glycerol saline solution for 15 months before testing by direct rapid immunohistochemical test (dRIT) and retested by direct fluorescent-antibody assay (DFA) after 5 months. A) Brain stained by dRIT: rabies virus antigen appears as magenta inclusions (arrowheads) against the blue neuronal hematoxylin counterstain. Magnification, ×630. B) Immunofluorescent apple-green viral inclusions in the same brain processed by DFA. Magnification, ×200.

Main Article

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