Volume 12, Number 2—February 2006
Synopsis
Bartonella quintana Characteristics and Clinical Management
Cédric Foucault*, Philippe Brouqui*, and Didier Raoult*
Table A1
Available primers for Bartonella spp. Amplification
| Microorganism | Gene | Direction* | Primer | Sequence |
|---|---|---|---|---|
| Bartonella clarridgeiae | ribC | F | PBC5 | TACATAACGAGCCAATT |
| B. clarridgeiae | ribC | R | PBC15 | TAGCTTTAGAACAATATGGT |
| B. henselae | ribC | F | PBH-L1 | GATATCGGTTGTGTTGAAGA |
| B. henselae | ribC | R | PBH-R1 | AATAAAAGGTATAAAACGCT |
| B. bacilliformis | ribC | F | PBH-L1 | GATATCGGTTGTGTTGAAGA |
| B. bacilliformis | ribC | R | PBB-R1 | AAAGGCGCTAACTGTTC |
| B. quintana | ribC | F | PBH-L1 | GATATCGGTTGTGTTGAAGA |
| B. quintana | ribC | R | PBQ-R1 | AAAGGGCGTGAATTTTG |
| All Bartonella | ribC | F | PBH3 | CCAAGTGCTACATAACCATC |
| All Bartonella | ribC | R | PBH4 | CGGGTTGTTATTGCTCTTAC |
| All (except B. bacilliformis) | 16s-23s spacer | F | 16SF | AGAGGCAGGCAACCACGGTA |
| All (except B. bacilliformis) | 16s-23s spacer | R | 23S1 | GCCAAGGCATCCACC |
| All Bartonella | 16s-23s spacer | F | QHVE1 | TTCAGATGATGATCCCAA |
| All Bartonella | 16s-23s spacer | R | QHVE2 | TTGGGATCATCATCTGAA |
| All Bartonella | 16s-23s spacer | F | QHVE3 | GATATATTCAGACATGTT |
| All Bartonella | 16s-23s spacer | R | QHVE4 | AACATGTCTGAATATATC |
| B. bacilliformis | 16s-23s spacer | F | BABF | CTGGATCACCTCCTTTCTAA |
| B. bacilliformis | 16s-23s spacer | R | BABR | ATGCCCTTAAGACACTTGAT |
| B. quintana | 16s-23s spacer | F | BQF | CTCCACCATTTTAGGTCATC |
| B. quintana | 16s-23s spacer | R | BQR | GGTTTTGAGAATTCCCTTGC |
| All Bartonella | 16s-23s spacer | F | 16s1 | (C/T)CTTCGTTTCTCTTTCTTCA |
| All Bartonella | 16s-23s spacer | R | 16s2 | GGATAAACCGGAAAACCTTC |
| All Bartonella | 16s-23s spacer | F | 16s3 | (C/T)CTTCGTTTCTCTTTCTTCA |
| All Bartonella | 16s-23s spacer | R | 16s4 | AACCAACTGAGCTACAAGCC |
| All Bartonella | 16s-23s spacer | F | 16s5 | CTCTTTCTTCAGATGATGATCC |
| All Bartonella | 16s-23s spacer | R | 16s6 | AACCAACTGAGCTACAAGCCCT |
| All Bartonella | ribC | F | BARTON-1F | TAACCGATATTGGTTGTGTTGAAG |
| All Bartonella | ribC | R | BARTON-2R | TAAAGCTAGAAAGTCTGGCAACATAACG |
| All Bartonella | 16s | S | probe | ATTTGGTTGGGCACTCTAGGGG |
| All Bartonella | 16s | Rp2a | ACGGCTACCTTGTTAGGACTT | |
| All Bartonella | 16s | F | 357f | TACGGGAGGCAGCAG |
| All Bartonella | 16s | R | 357ra | CTGCTGCCTCCCGTA |
| All Bartonella | 16s | F | 536F | CAGCAGCCGCGGTAATAC |
| All Bartonella | 16s | R | 536R | GTATTACCGCGGCTGCTG |
| All Bartonella | 16s | F | 800F | ATTAGATACCCTGGTAG |
| All Bartonella | 16s | R | 800R | CTACCAGGGTATCTAAT |
| All Bartonella | 16s | F | 1050F | TGTCGTCAGCTCGTG |
| All Bartonella | 16s | R | 1050R | CACGAGCTGACGACA |
| All Bartonella | 35KD | F | 35KD1fa | GTCGCTAAAGGCTGATGA |
| All Bartonella | 35KD | R | 35KD2ra | GACTGATATCGTGCGTGTG |
| All Bartonella | 35KD | F | 35KDs1f | GGTACGACGACAGTAATTGTT |
| All Bartonella | 35KD | R | 35KDs2r | GATTTAAGAGATACCAACCA |
| All Bartonella | PAP | F | PAP1fa | CTTTAATGACGACTTCTGTT |
| All Bartonella | PAP | R | PAP4ra | CCGAAATCTGAGTAACGGTA |
| All Bartonella | PAP | R | PAP2r | CCCTAAATGTTTCAAGTTCA |
| All Bartonella | PAP | F | PAP3f | GCTGACAGAGAAGACGCAA |
| All Bartonella | groEL | F | BbHs233.p | CGTGAAGTTGCCTCAAAAACC |
| All Bartonella | groEL | R | BbHs1630.n | AATCCATTCCGCCCATTC |
| All Bartonella | rpoB | F | QVE1 | TTCAGATGATGATCCCAAGC |
| All Bartonella | rpoB | R | QVE3 | AACATGTCTGAATATATCTTC |
| All Bartonella | Bfp1 | ATTAATCTGCAYCGGCCAGA | ||
| All Bartonella | Bfp2 | ACVGADACACGAATAACACC | ||
| All Bartonella | Bfs3 | TTACAAAAATCYGTTGATAC | ||
| All Bartonella | Bfs4 | GTATCAACRGATTTTTGTAA | ||
| All Bartonella | ftsZ | F | BaftsZF | GCTAATCGTATTCGCGAAGAA |
| All Bartonella | ftsZ | R | BaftsZR | GCTGGTATTTCCAAYTGATCT |
| B. henselae, B. clarridgeiae | ftsZ | BhftsZ 1393.n | GCGAACTACGGCTTACTTGC | |
| B. henselae, B. clarridgeiae | ftsZ | Bh ftsZ 1247.p | CGGTTGGAGAGCAGTTTCGTC | |
| B. quintana | ftsZ | F | Bq ftsZseqF | GCACATATTCTTGATGAGAT |
| B. quintana | ftsZ | R | Bq ftsZseqR | CCCCTATCATCTCATCAAG |
| B. bacilliformis | ftsZ | F | Bb ftsZseqF | GCGCATGTTCTTAGTGAAAT |
| B. bacilliformis | ftsZ | R | Bb ftsZseqR | CCTGTATACGTGATGCATTT |
| All Bartonella | ftsZ | FTS1p | GCCTTCTCATCCTCAACTT | |
| All Bartonella | ftsZ | FTS2p | CAGCCTCTTCACGATGTG | |
| All Bartonella | pap31 | PAPn1 | TTCTAGGAGTTGAAACCGAT | |
| All Bartonella | pap31 | PAPn2 | GAAACACCACCAGCAACATA | |
| All Bartonella | pap31 | PAPns2 | GCACCAGACCATTTTTCCTT | |
| All Bartonella | pap31 | PAPns1 | CAGAGAAGACGCAAAAACCT | |
| All Bartonella | groEL | HSPps1 | CAGAAGTTGAAGTGAAAGAAAA | |
| All Bartonella | groEL | HSPps2 | GCNGCTTCTTCACCNGCATT | |
| All Bartonella | groEL | HSPps4 | GCTGGNGGTGTTGCNGTTA | |
| All Bartonella | groEL | HSPps3 | GCTGTNGAAGANGGNATTGT |
*F, forward; R, reverse.
Page created: February 02, 2012
Page updated: February 02, 2012
Page reviewed: February 02, 2012
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.