Volume 12, Number 3—March 2006
Dispatch
Pneumonia and New Methicillin-resistant Staphylococcus aureus Clone
Fabien Garnier*
, Anne Tristan†, Bruno François‡, Jerome Etienne†, Manuella Delage-Corre§, Christian Martin*, Nadia Liassine¶, Wim Wannet#, François Denis*, and Marie-Cécile Ploy*
, Anne Tristan†, Bruno François‡, Jerome Etienne†, Manuella Delage-Corre§, Christian Martin*, Nadia Liassine¶, Wim Wannet#, François Denis*, and Marie-Cécile Ploy*
Table
Primers used to screen for staphylococcal toxin genes, agr alleles, and mecA gene
| Gene | Primer | Oligonucleotide sequence (5´–3´) | Reference |
|---|---|---|---|
| mecA | mecA-1 | AAAATCGATGGTAAAGGTTGGC | (6) |
| mecA-2 | AGTTCTGCAGTACCGGATTTGC | ||
| agr1 | agr1-1 | ATGCACATGGTGCACATGC | (5) |
| agr1-2 | GTCACAAGTACTATAAGCTGCGAT | ||
| agr2 | agr2-1 | ATGCACATGGTGCACATGC | (5) |
| agr2-2 | TATTACTAATTGAAAAGTGCCATAGC | ||
| agr3 | agr3-1 | ATGCACATGGTGCACATGC | (5) |
| agr3-2 | GTAATGTAATAGCTTGTATAATAATACCCAG | ||
| agr4 | agr4-1 | ATGCACATGGTGCACATGC | (5) |
| agr4-2 | CGATAATGCCGTAATACCCG | ||
| sea | sea-1 | GAAAAAAGTCTGAATTGCAGGGAACA | (3) |
| sea-2 | CAAATAAATCGTAATTAACCGAAGGTTC | ||
| seb | seb-1 | ATTCTAATTAAGGACACTAAGTTAGGGA | (3) |
| seb-2 | ATCCCGTTTCATAAGGCGAGT | ||
| sec | sec-1 | GTAAAGTTACAGGTGGCAAAACTTG | (3) |
| sec-2 | CATATCATACCAAAAAGTATTGCCGT | ||
| sed | sed-1 | GAATTAAGTAGTACCGCGCTAAATAATATG | (3) |
| sed-2 | GCTGTATTTTTCCTCCGAGAGT | ||
| see | see-1 | CAAAGAAATGCTTTAAGCAATCTTAGGC | (3) |
| see-2 | CACCTTACCGCCAAAGCTG | ||
| tsst | tsst-1 | TTCACTATTTGTAAAAGTGTCAGACCCACT | (3) |
| tsst-2 | TACTAATGAATTTTTTTATCGTAAGCCCTT | ||
| eta | eta-1 | ACTGTAGGAGCTAGTGCATTTGT | (3) |
| eta-2 | TGGATACTTTTGTCTATCTTTTTCATCAAC | ||
| etb | etb-1 | CAGATAAAGAGCTTTATACACACATTAC | (3) |
| etb-2 | AGTGAACTTATCTTTCTATTGAAAAACACTC | ||
| LukS-PV/lukF-PV | pvl-1 | ATCATTAGGTAAAATGTCTGGACATGATCCA | (3) |
| Npvl-2 | GCATCAASTGTATTGGATAGCAAAAGC |
References
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