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Volume 12, Number 3—March 2006
Dispatch

Pneumonia and New Methicillin-resistant Staphylococcus aureus Clone

Fabien Garnier*Comments to Author , Anne Tristan†, Bruno François‡, Jerome Etienne†, Manuella Delage-Corre§, Christian Martin*, Nadia Liassine¶, Wim Wannet#, François Denis*, and Marie-Cécile Ploy*
Author affiliations: *Laboratoire de Bactériologie-Virologie-Hygiène, Limoges, France; †Centre National de Reference des Staphylocoques, Lyon, France; ‡Service de Réanimation Polyvalente, Limoges, France; §Service d'Anatomie Pathologique, Limoges, France; ¶Bioanalytique-Riotton, Geneva, Switzerland; #National Institute of Public Health and the Environment, Bilthoven, the Netherlands

Main Article

Table

Primers used to screen for staphylococcal toxin genes, agr alleles, and mecA gene

Gene Primer Oligonucleotide sequence (5´–3´) Reference
mecA mecA-1 AAAATCGATGGTAAAGGTTGGC (6)
mecA-2 AGTTCTGCAGTACCGGATTTGC
agr1 agr1-1 ATGCACATGGTGCACATGC (5)
agr1-2 GTCACAAGTACTATAAGCTGCGAT
agr2 agr2-1 ATGCACATGGTGCACATGC (5)
agr2-2 TATTACTAATTGAAAAGTGCCATAGC
agr3 agr3-1 ATGCACATGGTGCACATGC (5)
agr3-2 GTAATGTAATAGCTTGTATAATAATACCCAG
agr4 agr4-1 ATGCACATGGTGCACATGC (5)
agr4-2 CGATAATGCCGTAATACCCG
sea sea-1 GAAAAAAGTCTGAATTGCAGGGAACA (3)
sea-2 CAAATAAATCGTAATTAACCGAAGGTTC
seb seb-1 ATTCTAATTAAGGACACTAAGTTAGGGA (3)
seb-2 ATCCCGTTTCATAAGGCGAGT
sec sec-1 GTAAAGTTACAGGTGGCAAAACTTG (3)
sec-2 CATATCATACCAAAAAGTATTGCCGT
sed sed-1 GAATTAAGTAGTACCGCGCTAAATAATATG (3)
sed-2 GCTGTATTTTTCCTCCGAGAGT
see see-1 CAAAGAAATGCTTTAAGCAATCTTAGGC (3)
see-2 CACCTTACCGCCAAAGCTG
tsst tsst-1 TTCACTATTTGTAAAAGTGTCAGACCCACT (3)
tsst-2 TACTAATGAATTTTTTTATCGTAAGCCCTT
eta eta-1 ACTGTAGGAGCTAGTGCATTTGT (3)
eta-2 TGGATACTTTTGTCTATCTTTTTCATCAAC
etb etb-1 CAGATAAAGAGCTTTATACACACATTAC (3)
etb-2 AGTGAACTTATCTTTCTATTGAAAAACACTC
LukS-PV/lukF-PV pvl-1 ATCATTAGGTAAAATGTCTGGACATGATCCA (3)
Npvl-2 GCATCAASTGTATTGGATAGCAAAAGC

Main Article

References
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