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Volume 12, Number 4—April 2006
Research

Lineage 1 and 2 Strains of Encephalitic West Nile Virus, Central Europe

Tamás Bakonyi*†, Éva Ivanics‡, Károly Erdélyi‡, Krisztina Ursu‡, Emőke Ferenczi§, Herbert Weissenböck*, and Norbert Nowotny*¶Comments to Author 
Author affiliations: *University of Veterinary Medicine, Vienna, Austria; †Szent István University, Budapest, Hungary; ‡Central Veterinary Institute, Budapest, Hungary; §"Béla Johan" National Center for Epidemiology, Budapest, Hungary; ¶United Arab Emirates University, Al Ain, United Arab Emirates

Main Article

Table A1

Oligonucleotide primers used for sequencing West Nile virus (WNV) strain Goose-Hungary/03*

Name* Sequence 5´ to 3´ Product length (bp)
WNVI 1f AGTAGTTCGCCTGTGTGAGC 388
WNVI 388r GCCGATTGATAGCACTGGTC
WNVI 82f AGCACGAAGATCTCGATGTC 986
WNVI 1067r ATGATAGTCACGCAGCTGTC
WNVI 1005f AGGAGTGTCTGGAGCAACAT 995
WNVI 1999r AAGCCACTGACGAGATAGGA
WNVI 1864f ACAACCTATGGCGTCTGTTC 1,049
WNVI 2912r CGATTCTGAGTCGGACATTC
WNVI 2847f AGAACTCGCCAACAACACCT 774
WNVI 3620r ATCTTGGCTGTCCACCTCTT
WNVI 3502f CTCGTGCAGTCACAAGTGAA 957
WNVI 4458r ATCAAGCCGCACATCAACTC
WNVI 4524f GGTCTGTCTCGCGATTAGTG 500
WNVI 5023r GTGAGCCTGATGTTCCAGTG
WNVI 5300f CTGAGATGGCTGAAGCACTG 858
WNVI 6157r TCTCACGCTCTGGTTGGTAG
WNVI 5303f AGATGGCTGAAGCACTGAGA 162
WNVI 5464r CCATCACGAACAGGTTGTAG
WNVI 6009f GTCGCAAGTTGGTGATGAGT 228
WNVI 6236r TCTGCAGTCCTCAACAGTTC
WNVI 6119f ACGGACTGATCGCTCAATTC 1,151
WNVI 7269r AAGGAGTGTTGCCGCTGTTA
WNVI 7177f TTCGTCGATGTTGGAGTGTC 993
WNVI 8169r CCGAATCGTCCTATGCTCTT
WNVI 8112f TTGTGACATCGGAGAGTCCT 1068
WNVI 9179r AGCCAGTGGTCTTCATTGAG
WNVI 8950f GCCAGAGAAGCAGTTGAAGA 305
WNVI 9254r CCAACTTCACGCAGGATGTA
WNVI 9134f TTCTGGAGTTCGAGGCTCTG 1,186
WNVI 10319r CCGATGATTGCTCTGACTTG
WNVI 9445f GGAAGAACCGTCATGGATGT 466
WNVI 9910r GAGCTCTGCCTACCAATTCA
WNVI 10524f CGCCACCGGAAGTTGAGTAG 501
WNVI 11024r CTGTGTTCTCGCACCACCAG

*Numbers refer to nucleic acid positions at the 5´-end of the primers, according to the complete genomic sequence of the closely related WNV strain WN NY 2000-crow3356 (AF404756 27; ). f, forward (genomic) primer; r, reverse (complementary) primer.

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