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Volume 12, Number 4—April 2006
Research

Lineage 1 and 2 Strains of Encephalitic West Nile Virus, Central Europe

Tamás Bakonyi*†, Éva Ivanics‡, Károly Erdélyi‡, Krisztina Ursu‡, Emőke Ferenczi§, Herbert Weissenböck*, and Norbert Nowotny*¶Comments to Author 
Author affiliations: *University of Veterinary Medicine, Vienna, Austria; †Szent István University, Budapest, Hungary; ‡Central Veterinary Institute, Budapest, Hungary; §"Béla Johan" National Center for Epidemiology, Budapest, Hungary; ¶United Arab Emirates University, Al Ain, United Arab Emirates

Main Article

Table A2

Oligonucleotide primers used for sequencing of West Nile virus (WNV) strain goshawk-Hungary/04

Name* Sequence 5´ to 3´ Product length (bp)
WNVII 1f AGTAGTTCGCCTGTGTGAGC 200
WNVII 200r AGCATAGCCCTCTTCAGTCC
WNVII 81f TGGCACGAAGATCTCGATGT 874
WNVII 954r TGCCACCAGGAGCAATAGAA
WNVII 870f CCTCGTTGCAGCTGTCATTG 761
WNVII 1630r TCCATGGCAGGTTCAGATCC
WNVII 1584f CTTCCTGGTTCACCGAGAAT 908
WNVII 2491r CTTGCCTGCCAATGTCAATG
Usu 2328f CTCTTTGGRGGRATGTCHTG 818
Usu 3145r GTGCADGATTTKACYTCTCC
WNVII 3027f CAACATGGCTGTGCATAGTG 673
WNVII 3699r GCCGACGAGAATGACATATC
Usu 3606f AAGAGGTGGACGGCCARRHT 1,152
Usu 4759r GTGTGCCAYAGYGTGTGGAA
WNVII 3861f GGCTTACTATGACGCCAAGA 594
WNVII 4454r CCATCATCATCCAGCCTAAC
Usu 4251f ATGTTYGCCATYGTWGGDGG 1,142
Usu 5392r TGGCACATSACATCMACKAT
WNVII 5294f TTGCTGCTGAGATGTCTGAG 324
WNVII 5617r TGTCCGAGATAGGAGCATTG
Usu 5454f ATGGATGAAGCYCATTTCAC 337
Usu 5790r GGTAYTCYGTMTCATAGGAC
WNVII 5629f GCCTGGAACACTGGATATGA 616
WNVII 6245r TAAGCGAGCCAGACTGGTAA
WNVII 6127f TATCAGCCTGAGCGCGAGAA 932
WNVII 7058r ACGGCTGTCGTTACGGCATA
WNVII 6935f ATGACATTGGCAGCCTGTTG 672
WNVII 7606r ATCCTCCTCGCATGATGTGA
WNVII 7505f GAGAGGCTGGAATTCTGACT 653
WNVII 8158r GTTCTTCTACCTCGGCACTT
WNV 8078f CATCAGAAGCGAGCGACACA 744
WNV 8821r ACAGCCAGTTCGTGGTCTCA
WNV 8723f TCGGTCAACAACGAGTGTTC 739
WNV 9461r GAGATGACGTCCATCACAGT
WNV 9368f TAGCGCGGTCCATCATCGAG 733
WNV 10100r CCAACGTTCGACCGGAGTCT
WNVII 10023f CTGTTCCGCTGTGCCTGTCA 519
WNVII 10541r CGTGGATCACCTCGCAGCTT
WNV 9031f TACAACATGATGGGVAARAGAGAGA 1,061
WNV 10091r AGCATGTCTTCYGTBGTCATCCAYT
WNV 10460f GCCACCGGAAGTTGAGTAGA 430
WNV 10889r GCTGGTTGTGCAGAGCAGAA
Usu 10596f GWAAGCCTCYCAGAACCGTCTCGGAAG 419
Usu 11014r AGATCCTGTGKTCTWSYYCMCCAYCAG

*Numbers refer to nucleic acid positions at the 5´-end, according to the complete genomic sequence of the closely related lineage 2 WNV strain B956 (NC_001563). Universal, Japanese encephalitis virus–group specific primers are indicated in italics (28). f, forward (genomic) primer; r, reverse (complementary) primer. Usu, Usutu.

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Page updated: January 24, 2012
Page reviewed: January 24, 2012
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