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Volume 12, Number 6—June 2006


Mixed Cryptosporidium Infections and HIV

Vitaliano Cama*†, Robert H. Gilman†‡, Aldo Vivar§, Eduardo Ticona¶, Ynes Ortega#, Caryn Bern*, and Lihua Xiao*Comments to Author 
Author affiliations: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA; †Johns Hopkins University, Baltimore, Maryland, USA; ‡Asociación Benéfica Proyectos en Informática, Salud, Medicina y Agricultura, Lima, Peru; §Hospital Arzobispo Loayza, Lima, Peru; ¶Hospital Dos de Mayo, Lima, Peru; #University of Georgia, Griffin, Georgia, USA

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Results of multilocus genotyping of Cryptosporidium specimens originally diagnosed as Cryptosporidium canis and C. felis by an SSU rRNA–based PCR-RFLP tool*

Participant No. specimens tested No. days between first and last specimen Cryptosporidium genotype by locus (no. specimens)
SSU rRNA COWP DHFR Mixed infection
0043D 7 29 C. canis No
0214D 2 5 C. canis No
0448D 4 45 C. canis C. hominis (1) and C. meleagridis (2) C. hominis (2) and C. meleagridis (2) Yes
1083D 1 C. canis No
1322D 2 2 C. canis No
0002D 1 C. canis No
0034D 7 56 C. canis C. parvum (2) C. parvum (2) Yes
0482D 1 C. canis C. hominis (1) C. hominis (1) Yes
0500D 1 C. canis No
0533D 3 3 C. canis No
0670D 4 414† C. canis C. hominis (4) C. hominis (2) Yes
0725D 1 C. canis No
0044A 1 C. felis C. meleagridis (1) C. meleagridis (1) Yes
0076A 4 31 C. felis C. hominis (3) C. hominis (1) Yes
0668A 3 3 C. felis C. hominis (1) C. hominis (2) Yes
0673A 5 31 C. felis No
0817A 2 2 C. felis No
0891A 1 C. felis No
1344A 3 3 C. felis No
0569D 2 2 C. felis No
0776D 1 C. felis No

*PCR-RFLP, polymerase chain reaction–restriction fragment length polymorphism; SSU, small subunit; COWP, Cryptosporidium oocyst wall protein; DHFR, dihydrofolate reductase gene.
†Specimens correspond to 2 visits 14 months apart.

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