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Volume 12, Number 7—July 2006

European Bat Lyssavirus Type 2 RNA in Myotis daubentonii

Nicholas Johnson*Comments to Author , Philip R. Wakeley*, Sharon M. Brookes*, and Anthony R. Fooks*
Author affiliations: *Veterinary Laboratories Agency, Addlestone, Surrey, United Kingdom

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Figure. Results of quantitative polymerase chain reaction (PCR) of viral genome copies within organ samples taken from 2 Daubenton's bats infected with European bat lyssavirus type 2 (EBLV-2). A) Standard curve of duplicate dilutions of known quantities of EBLV-2 amplicon. A 20% (vol/vol) sample (10-fold dilution series) separated by electrophoresis on a 1% agarose gel is shown in the inset. Ct, threshold value. B) Real-time PCR amplification of EBLV-2 genomic RNA from organ samples from a bat (696/04) in Lancashire, United Kingdom infected with EBLV-2. dR, fluorescence change generated by a baseline-corrected algorithm calculated from every amplification cycle. A 20% (vol/vol) sample of each amplification separated by electrophoresis on a 1% agarose gel is shown in the inset. Br, brain; To, tongue; Li, liver; Ki, kidney; In, intestine; St, stomach; -, negative sample (no template); +, positive sample from a previous human case (5).

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