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Volume 12, Number 9—September 2006
Research

Differentiation of Tuberculosis Strains in a Population with Mainly Beijing-family Strains

Vladyslav Nikolayevskyy*, Krishna Gopaul*, Yanina Balabanova*†, Timothy Brown*, Ivan Fedorin†, and Francis Drobniewski*Comments to Author 
Author affiliations: *Barts and the London School of Medicine, University of London, London, United Kingdom; †Samara Regional Tuberculosis Service, Samara, Russia

Main Article

Table A1

Extended panel of loci, primer sequences, and PCR conditions for VNTR-MIRU analysis*

Markers
 Primer sequences
H37Rv locus name MIRU system† (1,2) ETR system
 (3) Mtub system
 (4) QUB system (5,6) Forward Reverse Label
1 0154 MIRU2† 5´-CAG GTG CCC TAT CTG CTG ACG-3´ 5´-GTT GCG TCC GGC ATA CCA AC-3´ Dye 3
2 0424 Mtub4 5´-CCG CCC TGG TCG TCT GGA-3´ 5´-CGG CAT CCT CAA CAA CGG TAG C-3´ Dye 3
3 0531 MPTR-A 5´-CTG CCG AGG CCC GCG TTG ATT GT-3´ 5´-GAC GTG GCG GCG ATG GCT GGT TA-3´ Dye 2
4 0577 ETR-C‡ 5´-GTG AGT CGC TGC AGA ACC TGC AG-3´ 5´-GGC GTC TTG ACC TCC ACG AGT G-3´ Dye 3
4 0580 MIRU4 EDR-D 5´-GTC AAA CAG GTC ACA ACG AGA GGA A-3´ 5´-CCT CCA CAA TCA ACA CAC TGG TCA T-3´ Dye 3
5 0802 MIRU40 5´-GAT TCC AAC AAG ACG CAG ATC AAG A-3´ 5´-TCA GGT CTT TCT CTC ACG CTC TCG-3´ Dye 3
6 0959 MIRU10 5´-ACC GTC TTA TCG GAC TGC ACT ATC AA-3´ 5´-CAC CTT GGT GAT CAG CTA CCT CGA T-3´ Dye 4
7 1644 MIRU16 5´-CGG GTC CAG TCC AAC TAC CTC AAT-3´ 5´-GAT CCT CCT GAT TGC CCT GAC CTA-3´ Dye 2
8 1955 Mtub21 5´-TGT CGA GTT CAC CGT CCA TCA TCT-3´ 5´-CCG ACG CCA ATA GCA CAG CAC CAG-3´ Dye 4
9 1982 QUB-18 5´-GGA ATG GCT ACG GAA GGA ATA CTC-3´ 5´-TTA CGA CAC CTG ATC TGA CTC TGC- 3´ Dye 2
10 2059 MIRU20 5´-CCC CTT CGA GTT AGT ATC GTC GGT T-3´ 5´-CAA TCA CCG TTA CAT CGA CGT CAT C-3´ Dye 2
11 2074 Mtub24 5´-CGC GAG GAC GAG GTG GAG AA-3´ 5´-ACA ATT GCA GCC AGA GAT GAG ACG-3´ Dye 4
12 2163a QUB-11a 5´-CCC GGG GCG CTC GTG ATG- 3´ 5´-CGG CGG CAC CCT GGA GTC TGG-3´ Dye 4
13 2165 ETR-A‡ 5´-AAA TCG GTC CCA TCA CCT TCT TAT-3´ 5´-CGA AGC CTG GGG TGC CCG CGA TTT-3´ Dye 2
14 2387 MIRU24 5´-GAA GGC TAT CCG TCG ATC GGT T-3´ 5´-GGG CGA GTT GAG CTC ACA GAA C-3´ Dye 3
15 2461 ETR-B‡ 5´-GCG AAC ACC AGG ACA GCA TCA TG-3´ 5´-GGC ATG CCG GTG ATC GAG TGG-3´ Dye 4
16 2531 MIRU23 5´-CGA ATT CTT CGG TGG TCT CGA GT-3´ 5´-ACC GTC TGA CTC ATG GTG TCC AA-3´ Dye 4
17 2996 MIRU26 5´-GCG GAT AGG TCT ACC GTC GAA ATC-3´ 5´-TCC GGG TCA TAC AGC ATG ATC A-3´ Dye 4
18 3006 MIRU27 QUB-5 5´-TCT GCT TGC CAG TAA GAG CCA-3´ 5´-GTG ATG GTG ACT TCG GTG CCT T-3´ Dye 4
19 3192 MIRU31 ETR-E 5´-CGT CGA AGA GAG CCT CAT CAA TCA T-3´ 5´-AAC CTG CTG ACC GAT GGC AAT ATC-3´ Dye 3
20 3232 QUB-3232 5´-CAC TAG TTG TTG CGG CGA TGG T-3´ 5´-AAG GGC GGC ATT GTG TTC C-3´ Dye 3
21 3239 ETR-F 5´-GAC TTC GGG CAG CTC GGG CAT CC-3´ 5´-CCG CGG TGG TTG TCG TGA TG-3´ Dye 2
22 3336 QUB-3336 5´-GAT CGG GTG CAG TGG TTT CAG GTG-3´ 5´-GGG CGG CCA GCG GTG TC-3´ Dye 3
23 3690 Mtub39 5´-CGA GGA TCA CGA TGC GGG TCA C-3´ 5´-GGC GGG GGC TCG GGT GGT A-3´ Dye 4
24 4348 MIRU39 5´-CGG TCA AGT TCA GCA CCT TCT ACA TC-3´ 5´-GCG TCC GTA CTT CCG GTT CAG-3´ Dye 2

*VNTR, variable number tandem repeats; MIRU, mycobacterium interspersed repetitive units, ETR, exact tandem repeats.
†Primer sequences by Kwara et al. (7).
‡Primer sequences by Frothingham et al. (3).

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