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Volume 13, Number 1—January 2007

Spread of Cryptococcus gattii in British Columbia, Canada, and Detection in the Pacific Northwest, USA

Laura MacDougall*Comments to Author , Sarah E. Kidd†, Eleni Galanis*, Sunny Mak*, Mira J. Leslie‡, Paul R. Cieslak§, James W. Kronstad†, Muhammad G. Morshed*, and Karen H. Bartlett†
Author affiliations: *British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada; †University of British Columbia, Vancouver, British Columbia, Canada; ‡Washington State Department of Health, Shoreline, Washington, USA; §Oregon State Public Health, Portland, Oregon, USA;

Main Article

Table 4

Multilocus sequence typing (MLST) profiles of representative VGII strains from Vancouver Island compared with Cryptococcus gattii isolates from clinical and environmental sources in other locations*

Culture no.OriginSourceRFLP genotypeMLST profiles
A1M R265VIHumanVGIIa5†3†4†2†1†1†
A1M R272VIHumanVGIIb7‡343‡2‡2‡
A5M R57LMHumanVGIIa534211
A5M F738LMHumanVGIIa534211
KB14489WAFence postVGIIa534211
A6M R38ORHumanVGIIb11¶34#1

*RFLP, restriction fragment length polymorphism; VI, Vancouver Island; LM, British Columbia lower mainland; GI, Gulf Islands; WA, northern Washington, USA; OR, Oregon, USA.
†Representative sequence accession nos.: URA5 = AY973136, LAC = AY973094, FTR1 = AY972024, CAP1 = AY971991, PLB1 = DQ777861, IGS = DQ777859.
‡ Representative sequence accession nos.: URA5 = AY973141, CAP1 = AY971981, PLB1 = DQ777862, IGS = DQ777860.
§Accession no. for unique sequences: URA5 = DQ777864.
¶Accession no. for unique sequences: URA5 = DQ777863, FTR1 DQ777857, IGS DQ777858.
#Representative sequence accession no.: CAP1 = AY971973.

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