Volume 13, Number 1—January 2007
Research
Spread of Cryptococcus gattii in British Columbia, Canada, and Detection in the Pacific Northwest, USA
, Sarah E. Kidd†, Eleni Galanis*, Sunny Mak*, Mira J. Leslie‡, Paul R. Cieslak§, James W. Kronstad†, Muhammad G. Morshed*, and Karen H. Bartlett†
Table 4
Multilocus sequence typing (MLST) profiles of representative VGII strains from Vancouver Island compared with Cryptococcus gattii isolates from clinical and environmental sources in other locations*
| Culture no. | Origin | Source | RFLP genotype | MLST profiles | |||||
|---|---|---|---|---|---|---|---|---|---|
| URA5 | LAC | FTR1 | CAP1 | PLB1 | IGS | ||||
| A1M R265 | VI | Human | VGIIa | 5† | 3† | 4† | 2† | 1† | 1† |
| A1M R272 | VI | Human | VGIIb | 7‡ | 3 | 4 | 3‡ | 2‡ | 2‡ |
| A5M R57 | LM | Human | VGIIa | 5 | 3 | 4 | 2 | 1 | 1 |
| A5M F738 | LM | Human | VGIIa | 5 | 3 | 4 | 2 | 1 | 1 |
| KB7092 | LM | Animal | VGIIa | 5 | 3 | 4 | 2 | 1 | 1 |
| KB11765 | LM | Animal | VGIIa | 5 | 3 | 4 | 2 | 1 | 1 |
| KB2045 | LM | Air | VGIIa | 5 | 3 | 4 | 2 | 1 | 1 |
| KB13866 | GI | Soil | VGIIa | 5 | 3 | 4 | 2 | 1 | 1 |
| KB11377 | GI | Soil | VGIIa | 5 | 3 | 4 | 2 | 1 | 1 |
| KB14489 | WA | Fence post | VGIIa | 5 | 3 | 4 | 2 | 1 | 1 |
| KB14735 | WA | Soil | VGIIa | 5 | 3 | 4 | 2 | 1 | 1 |
| KB11632 | OR | Human | VGIIa | 12§ | 3 | 4 | 2 | 1 | 1 |
| A6M R38 | OR | Human | VGIIb | 11¶ | 3 | 9¶ | 4# | 1 | 3¶ |
*RFLP, restriction fragment length polymorphism; VI, Vancouver Island; LM, British Columbia lower mainland; GI, Gulf Islands; WA, northern Washington, USA; OR, Oregon, USA.
†Representative sequence accession nos.: URA5 = AY973136, LAC = AY973094, FTR1 = AY972024, CAP1 = AY971991, PLB1 = DQ777861, IGS = DQ777859.
‡ Representative sequence accession nos.: URA5 = AY973141, CAP1 = AY971981, PLB1 = DQ777862, IGS = DQ777860.
§Accession no. for unique sequences: URA5 = DQ777864.
¶Accession no. for unique sequences: URA5 = DQ777863, FTR1 DQ777857, IGS DQ777858.
#Representative sequence accession no.: CAP1 = AY971973.