Volume 13, Number 10—October 2007
Dispatch
Novel Variant of Tickborne Encephalitis Virus, Russia
Table 2
Individual amino acid substitutions of Glubinnoe/2004 and 205/Sofjin-HO viruses and unique substitutions in comparison with 37 flaviviruses, determined by polyprotein alignment*†
| Gene | Amino acid substitutions Glubinnoe/2004 → 205
or Sofjin-HO |
Unique substitutions | Changed putative cleavage sites | Summary of substitutions 205/Sofjin-HO | |
|---|---|---|---|---|---|
| 205 | Sofjin-HO | ||||
| Viral C | No | M43 → L; A54 → V; N64 → K | No | No | 0/3 |
| CTHD | A99‡ → V; I108 → V; M111 → I; M113‡ → V; F115‡ → L | A99‡ → V; I108 → V; M111 → L; M113‡ → V; F115‡ → L | No | Viral C/CTHD (RGKRR/SAA†DW) anchored C/prM (GM‡TF‡A/ATVRK) | 5/5 |
| prM | No | No | No | No | 0/0 |
| M | R210‡ → P | R210‡→ P; I246 → M; I266→ V | R210* | prM/M (SRTRR/SVLIR‡) | 1/3 |
| E | K508 → R; I597 → T; T646 → N; V743 → A | A433 → V; T646 → N; M740 → V; G759 → S | No | No | 4/4 |
| NS1 | G780‡ → C; K883 → R; S951 → P; I1053 → T | I1053 → T; V1122 → I | No | E/NS1 (LGVGA/DVGG‡A) | 4/2 |
| NS2A | R1180 → K; R1227 → S; G1250 → S; G1277 → E; C1311 → Y | R1180 → K; R1227 → S; G1250 → S; G1277 → E; I1296 → T; A1297 → V | No | No | 5/6 |
| NS2B | V1423 → M | V1423 → M | No | No | 1/1 |
| NS3 | E1563 → D; G1650 → E; I1673 → S; I1707 → T; T1828 → S; A1861 → V; P1948 → Q; V1975 → G; D1988 → N; A2062 → T | D1491‡ → G; S1551 → Y; E1563 → D; G1650 → E; I1673 → T; N1731 → S; T1828 → S; T1869 → A; P1948 → Q; V1975 → G; D1988 → N; A2062 → T | E1563; G1650; P1948; V1975; D1988 | NS2B/NS3 (RTARR/SD‡LVF) | 10/12 |
| NS4A | D2143 → E ;V2165 → A | D2143 → E; A2173 → G | D2143 | No | 2/2 |
| NS4B | M2283 → L; I2314 → M; A2331 → V; V2349 → I; A2472 → V | M2283 → V; F2347 → L; S2457 → A | M2283 | No | 5/3 |
| NS5 |
K2625 → R; A2757 → G;
G2758 → D; E3013 → G;
S3014 → F; G3033 → E;
K3074 → R; V3080 → I;
S3187 → G; P3251 → R;
V3260 → I; I3297 → V;
V3342 → I; K3389 → E;
Y3402 → D; N3406 → E |
K2526 → R; M2641 → V;
A2757 → G; G2758 → D;
E3013 → G; Y3030 → H;
V3080 → I; R3188 → G;
P3251 → R; I3297 → V;
V3342 → I; V3343 → A;
R3384 → K; K3389 → E;
Y3402 → D; N3406 → E |
A2757;
G2758;
P3251;
K3389;
Y3402;
N3406 |
No |
16/16 |
| Total | 14 | 5 | 53/57 | ||
*Position number corresponds to the polyprotein sequence Glubinnoe/2004 of tickborne encephalitis virus (TBEV). CTHD, C-terminal hydrophobic domain.
†The following polyprotein sequences were used for polyprotein alignment: DQ862460 (TBEV, Glubinnoe/2004); ABJ74160 (TBEV, 205); AAN73266 (TBEV, Senzhang); BAB71943 (TBEV, Oshima 5–10); BAB72162 (TBEV, Sofjin-HO); AF 069066 (TBEV, Vasilchenko);AAD34205 (TBEV, Vasilchenko); ABF46836 (TBEV, EK-238); AAO43537 (TBEV, Zausaev); NP_043135 (TBEV, Neudoerfl); AAZ80455 (TBEV, ts-variant of strain 263); AAA86739 (TBEV, 263); Q01299 (TBEV, Hypr); ABB90676 (Spanish sheep encephalitis virus); BB90675 (Turkish sheep encephalitis virus); AAQ91606 (OHFV, Bogolubovka); NP 878909 (OHFV, Bogoluvovska); AAR98531 (OHFV, Kubrin); ABB90677 (Greek goat encephalitis virus); NP_044677 (Louping ill virus);NP_620108 (Langat virus, TP21); AAF75260 (Langat virus, E5); P29837 (Langat virus, TP21);AAQ91607 (Kyasanur forest disease virus); NP_722551 (Alkhurma virus); NP_620099 (Powassan virus);AAL32169 (deer tick virus); ABB90669 (Gadgets Gully virus); ABB90671 (Karshi virus); YP_224133 (Karshi virus); ABE73208 (Karshi virus); ABB90673 (Royal Farm virus); ABB90670 (Kadam virus); ABB90674 (Saumarez Reef virus); ABB90668 (Meaban virus); ABB90672 (Tyuleniy virus); NP_689391). (Montana myotis leukoencephalitis virus).
‡For substitution in cleavage sites.