Volume 13, Number 12—December 2007
Dispatch
Parachlamydia spp. and Related Chlamydia-like Organisms and Bovine Abortion
Nicole Borel*, Silke Ruhl*, Nicola Casson†, Carmen Kaiser*, Andreas Pospischil*, and Gilbert Greub†
Table
Results of histologic, 16S rRNA sequence, and immunohistochemical analyses for 43 placentas positive for Chlamydia-like DNA by a 16S rRNA PCR*
| Specimen no. | Histology |
16S rRNA sequence† |
Immunohistochemistry |
|||||
|---|---|---|---|---|---|---|---|---|
| Placentitis | Vasculitis | Species | % Similarity | Parachlamydia spp. | Waddlia | |||
| 1 | N | Yes | Parachlamydia | 99 | + | – | ||
| 2 | N | No | Parachlamydia | 97 | + | – | ||
| 3 | P/N | No | Parachlamydia | 98 | + | – | ||
| 4 | P/N | No | Parachlamydia | 97 | – | – | ||
| 5 | P/N | No | Parachlamydia | 97 | – | – | ||
| 6 | A | No | Parachlamydia | 96 | + | – | ||
| 7 | A | No | Parachlamydia | 96 | + | – | ||
| 8 | A | No | Parachlamydia | 97 | + | – | ||
| 9 | P/N | Yes | Chlamydia-like | 92 | – | – | ||
| 10 | P/N | Yes | Chlamydia-like | 92 | – | – | ||
| 11 | P/N | Yes | Chlamydia-like | 93 | – | – | ||
| 12 | P/N | Yes | Chlamydia-like | 91 | + | – | ||
| 13 | P/N | No | Chlamydia-like | 82 | + | – | ||
| 14 | P/N | No | Chlamydia-like | 91 | + | – | ||
| 15 | P/N | No | Chlamydia-like | 92 | + | – | ||
| 16 | P/N | No | Chlamydia-like | 92 | + | – | ||
| 17 | P/N | No | Chlamydia-like | 92 | + | – | ||
| 18 | P/N | No | Chlamydia-like | 92 | + | – | ||
| 19 | P/N | No | Chlamydia-like | 92 | + | – | ||
| 20 | P/N | No | Chlamydia-like | 92 | + | – | ||
| 21 | P/N | No | Chlamydia-like | 93 | + | – | ||
| 22 | P/N | No | Chlamydia-like | 94 | + | – | ||
| 23 | P/N | No | Chlamydia-like | 95 | + | – | ||
| 24 | P/N | No | Chlamydia-like | 100 | + | – | ||
| 25 | P/N | No | Chlamydia-like | 93 | – | – | ||
| 26 | P/N | No | Chlamydia-like | 93 | – | – | ||
| 27 | P/N | No | Chlamydia-like | 95 | – | – | ||
| 28 | P/N | No | Chlamydia-like | 96 | – | – | ||
| 29 | N | No | Chlamydia-like | 85 | + | – | ||
| 30 | N | No | Chlamydia-like | 88 | + | – | ||
| 31 | N | No | Chlamydia-like | 88 | + | – | ||
| 32 | N | No | Chlamydia-like | 91 | + | – | ||
| 33 | N | No | Chlamydia-like | 91 | + | – | ||
| 34 | N | No | Chlamydia-like | 95 | + | – | ||
| 35 | P | No | Chlamydia-like | 91 | + | – | ||
| 36 | P | No | Chlamydia-like | 94 | + | – | ||
| 37 | A | No | Chlamydia-like | 91 | + | – | ||
| 38 | A | No | Chlamydia-like | 92 | + | – | ||
| 39 | A | No | Chlamydia-like | 92 | + | – | ||
| 40 | A | No | Chlamydia-like | 91 | – | – | ||
| 41 | A | No | Chlamydia-like | 92 | – | – | ||
| 42 | A | No | Chlamydia-like | 93 | – | – | ||
| 43 | A | No | Chlamydia-like | 95 | – | – | ||
*When partial 16S rRNA sequence showed a similarity >95% with a recognized species (i.e., Parachlamydia acanthamoebae), the corresponding genus was reported (i.e., Parachlamydia spp.). Conversely, when the sequence showed a best BLAST (www.ncbi.nlm.nih.gov) hit with uncultured or uncharacterized Chlamydia-related organisms, the sequence was designated as being similar to a Chlamydia-like organism. N, necrotizing; +, positive; –, negative; P, purulent; A, autolysis.
†A 278-bp fragment was amplified and sequenced (9).
References
- Aitken ID, Clarkson MJ, Linklater K. Enzootic abortion of ewes. Vet Rec. 1990;126:136–8.PubMedGoogle Scholar
- Pospischil A, Thoma R, Hilbe M, Grest P, Gebbers JO. Abortion in woman caused by caprine Chlamydophila abortus (Chlamydia psittaci serovar 1). Swiss Med Wkly. 2002;132:64–6.PubMedGoogle Scholar
- Longbottom D, Coulter LJ. Animal chlamydioses and zoonotic implications. J Comp Pathol. 2003;128:217–44. DOIPubMedGoogle Scholar
- Friedman MG, Dvoskin B, Kahane S. Infections with the Chlamydia-like microorganism Simkania negevensis, a possible emerging pathogen. Microbes Infect. 2003;5:1013–21. DOIPubMedGoogle Scholar
- Corsaro D, Greub G. Pathogenic potential of novel chlamydiae and diagnostic approaches to infections due to these obligate intracellular bacteria. Clin Microbiol Rev. 2006;19:283–97. DOIPubMedGoogle Scholar
- Greub G, Raoult D. Parachlamydiaceae: potential emerging pathogens. Emerg Infect Dis. 2002;8:625–30.PubMedGoogle Scholar
- Dilbeck PM, Evermann JF, Crawford TB, Ward AC, Leathers CW, Holland CJ, Isolation of a previously undescribed rickettsia from an aborted bovine fetus. J Clin Microbiol. 1990;28:814–6.PubMedGoogle Scholar
- Henning K, Schares G, Granzow H, Polster U, Hartmann M, Hotzel H, Neospora caninum and Waddlia chondrophila strain 2032/99 in a septic stillborn calf. Vet Microbiol. 2002;85:285–92. DOIPubMedGoogle Scholar
- Borel N, Thoma R, Spaeni P, Weilenmann R, Teankum K, Brugnera E, Chlamydia-related abortions in cattle from Graubunden, Switzerland. Vet Pathol. 2006;43:702–8. DOIPubMedGoogle Scholar
- Casson N, Entenza JM, Greub G. Serological cross-reactivity between different Chlamydia-like organisms. J Clin Microbiol. 2007;45:234–6. DOIPubMedGoogle Scholar
- Borel N, Mukhopadhyay S, Kaiser C, Sullivan ED, Miller RD, Timms P, Tissue MicroArray (TMA) analysis of normal and persistent Chlamydophila pneumoniae infection. BMC Infect Dis. 2006;6:152. DOIPubMedGoogle Scholar
- Greub G, Raoult D. Crescent bodies of Parachlamydia acanthamoeba and its life cycle within Acanthamoeba polyphaga: an electron micrograph study. Appl Environ Microbiol. 2002;68:3076–84. DOIPubMedGoogle Scholar
- Casson N, Medico N, Bille J, Greub G. Parachlamydia acanthamoebae enters and multiplies within pneumocytes and lung fibroblasts. Microbes Infect. 2006;8:1294–300. DOIPubMedGoogle Scholar
- Greub G, Mege JL, Raoult D. Parachlamydia acanthamoebae enters and multiplies within human macrophages and induces their apoptosis. Infect Immun. 2003;71:5979–85. DOIPubMedGoogle Scholar
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