Volume 13, Number 12—December 2007
Dispatch
Parachlamydia spp. and Related Chlamydia-like Organisms and Bovine Abortion
Table
Results of histologic, 16S rRNA sequence, and immunohistochemical analyses for 43 placentas positive for Chlamydia-like DNA by a 16S rRNA PCR*
Specimen no. | Histology |
16S rRNA sequence† |
Immunohistochemistry |
|||||
---|---|---|---|---|---|---|---|---|
Placentitis | Vasculitis | Species | % Similarity | Parachlamydia spp. | Waddlia | |||
1 | N | Yes | Parachlamydia | 99 | + | – | ||
2 | N | No | Parachlamydia | 97 | + | – | ||
3 | P/N | No | Parachlamydia | 98 | + | – | ||
4 | P/N | No | Parachlamydia | 97 | – | – | ||
5 | P/N | No | Parachlamydia | 97 | – | – | ||
6 | A | No | Parachlamydia | 96 | + | – | ||
7 | A | No | Parachlamydia | 96 | + | – | ||
8 | A | No | Parachlamydia | 97 | + | – | ||
9 | P/N | Yes | Chlamydia-like | 92 | – | – | ||
10 | P/N | Yes | Chlamydia-like | 92 | – | – | ||
11 | P/N | Yes | Chlamydia-like | 93 | – | – | ||
12 | P/N | Yes | Chlamydia-like | 91 | + | – | ||
13 | P/N | No | Chlamydia-like | 82 | + | – | ||
14 | P/N | No | Chlamydia-like | 91 | + | – | ||
15 | P/N | No | Chlamydia-like | 92 | + | – | ||
16 | P/N | No | Chlamydia-like | 92 | + | – | ||
17 | P/N | No | Chlamydia-like | 92 | + | – | ||
18 | P/N | No | Chlamydia-like | 92 | + | – | ||
19 | P/N | No | Chlamydia-like | 92 | + | – | ||
20 | P/N | No | Chlamydia-like | 92 | + | – | ||
21 | P/N | No | Chlamydia-like | 93 | + | – | ||
22 | P/N | No | Chlamydia-like | 94 | + | – | ||
23 | P/N | No | Chlamydia-like | 95 | + | – | ||
24 | P/N | No | Chlamydia-like | 100 | + | – | ||
25 | P/N | No | Chlamydia-like | 93 | – | – | ||
26 | P/N | No | Chlamydia-like | 93 | – | – | ||
27 | P/N | No | Chlamydia-like | 95 | – | – | ||
28 | P/N | No | Chlamydia-like | 96 | – | – | ||
29 | N | No | Chlamydia-like | 85 | + | – | ||
30 | N | No | Chlamydia-like | 88 | + | – | ||
31 | N | No | Chlamydia-like | 88 | + | – | ||
32 | N | No | Chlamydia-like | 91 | + | – | ||
33 | N | No | Chlamydia-like | 91 | + | – | ||
34 | N | No | Chlamydia-like | 95 | + | – | ||
35 | P | No | Chlamydia-like | 91 | + | – | ||
36 | P | No | Chlamydia-like | 94 | + | – | ||
37 | A | No | Chlamydia-like | 91 | + | – | ||
38 | A | No | Chlamydia-like | 92 | + | – | ||
39 | A | No | Chlamydia-like | 92 | + | – | ||
40 | A | No | Chlamydia-like | 91 | – | – | ||
41 | A | No | Chlamydia-like | 92 | – | – | ||
42 | A | No | Chlamydia-like | 93 | – | – | ||
43 | A | No | Chlamydia-like | 95 | – | – |
*When partial 16S rRNA sequence showed a similarity >95% with a recognized species (i.e., Parachlamydia acanthamoebae), the corresponding genus was reported (i.e., Parachlamydia spp.). Conversely, when the sequence showed a best BLAST (www.ncbi.nlm.nih.gov) hit with uncultured or uncharacterized Chlamydia-related organisms, the sequence was designated as being similar to a Chlamydia-like organism. N, necrotizing; +, positive; –, negative; P, purulent; A, autolysis.
†A 278-bp fragment was amplified and sequenced (9).
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