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Volume 13, Number 4—April 2007
Research

Global Emergence of Trimethoprim/Sulfamethoxazole Resistance in Stenotrophomonas maltophilia Mediated by Acquisition of sul Genes

Mark A. Toleman*, Peter M. Bennett*, David M.C. Bennett*, Ronald N. Jones†, and Timothy R. Walsh*Comments to Author 
Author affiliations: *School of Medicine at Cardiff University, Cardiff, Wales; †The JONES Group/JMI Laboratories, North Liberty, Iowa, USA;

Main Article

Table

Oligonucleotide primers used in this study, Cardiff, 2007

Primer Sequence (5′→3′) GenBank accession no. Target Reference
Ina-F
 GCCTGTTCGGTTCGTAAGCT
intI
 (14)
Int-R
 CGGATGTTGCGATTACTTCG
intI
 (14)
sul1-F
 ATGGTGACGGTGTTCGGCATTCTGA
sul1
 (15)
sul1-R
 CTAGGCATGATCTAACCCTCGGTCT
sul1
 (15)
sul2-F
 GAATAAATCGCTCATCATTTTCGG
 AJ289135
 sul2
 (15)
sul2-R
 CGAATTCTTGCGGTTTCTTTCAGC
 AJ289135
 sul2
 (15)
aacA4-F
 AACTTGCGAGCGATCCGATG
aacA4
 (14)
aacA4-R
 ATGTACACGGCTGGACCATC
aacA4
 (14)
aacA7-F
 AATGGATAGTTCGCCGCTCG
aacA7
 This study
aacA7-R
 TTCCGGAAGCAGCGTACTTG
aacA7
 This study
CRF
 CACTWCCACATGCTGTKKC
 AF231986
 All ISCR
 This study
CRF-r
 GMMACAGCATGTGGWAGTG
 AF231986
 All ISCR
 This study
CRFF
 GGRYGCAACGSCTCAAGCG
 AF231986
 All ISCR
 This study
CRFF-r
 CGCTTGAGSCGTTGCRYCC
 AF231986
 All ISCR
 This study
LECR2
 CACTGGCTGGCAATGTCTAG
 AF231986
 ISCR2
 This study
RECR2
 CTTTGGACCGCAGTTGACTC
 AF231986
 ISCR2
 This study
FloF
 TCGACATCCTGGCTTCACTG
 AF231986
 floR
 This study
FloR
 ATTACAAGCGCGACAGTGGC
 AF231986
 floR
 This study
dfra20f
 GGGAAACACCGAGAAATGGG
 AJ605332
 dfrA20
 This study
dfrA20R
 TTCTTCTTCCCATTCTCCCC
 AJ605332
 dfrA20
 This study
dfrA9F
 CAGATTCCGTGGCATGAACC
 X57730
 dfrA9
 This study
dfrA9R
 GACCTCAGATACGAGTTTCC
 X57730
 dfrA9
 This study
dfrA10F
 TGTAGCGCGTGGTGTAAACG
 AY055428
 dhfr10
 This study
dfrA10R
 ACGTCTACGTGAGTATCCGC
 AY055428
 dhfr10
 This study
strAF
 TCTGTCGCACCTGCTTGATC
 AY055428
 strA
 This study
strAR
 CATTGCTGATGAACTGCGCG
 AY055428
 strA
 This study
tetAF
 CGCTGTTTGTGATTACACCC
 AJ250203
 tetA
 This study
tetAR
 CAGCGAGATGCGATATATCC
 AJ250203
 tetA
 This study
glmmR
 GAGTCAACTGCGGTCCAAAC
 AJ289135
 glmM
 This study
glmmF ACGGTATTCGTGGCAAAGCC AJ289135 glmM This study

Main Article

References
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Main Article

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Page updated: June 28, 2010
Page reviewed: June 28, 2010
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