Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 13, Number 6—June 2007
Letter

Ilheus Virus Isolate from a Human, Ecuador

Barbara W. Johnson*Comments to Author , Cristopher Cruz†, Vidal Felices†, William R. Espinoza‡, Stephen Robert Manock§, Carolina Guevara†, James G. Olson†, and Tadeusz J. Kochel†
Author affiliations: *Centers for Disease Control and Prevention, Fort Collins, Colorado, USA; †US Naval Medical Research Center Detachment, Lima, Peru; ‡Hospital de la IV División del Ejercito “Amazonas,” Puyo, Ecuador; §Hospital Vozandes del Oriente, Shell, Ecuador;

Main Article

Figure

Phylogenetic analysis of the NS5 (A) and E gene (B) regions of 6 Ilheus virus (ILHV) isolates. The sequences were aligned in MegAlign (DNASTAR, Inc., Madison, WI, USA); the alignments were then analyzed by using the maximum parsimony method with 500 bootstrap replicates in the program MEGA 3.1 (10). Rocio virus (GenBank accession no. AY632542) was included as an outgroup in the analysis, based on the phylogram of Kuno and Chang (1). Bootstrap values, shown in boldface above the branch, are percentages derived from 500 samplings; branch lengths are shown below the branch. The sequences generated from our study were deposited in GenBank under accession nos. EF396941–EF396952.

Figure. Phylogenetic analysis of the NS5 (A) and E gene (B) regions of 6 Ilheus virus (ILHV) isolates. The sequences were aligned in MegAlign (DNASTAR, Inc., Madison, WI, USA); the alignments were then analyzed by using the maximum parsimony method with 500 bootstrap replicates in the program MEGA 3.1 (10). Rocio virus (GenBank accession no. AY632542) was included as an outgroup in the analysis, based on the phylogram of Kuno and Chang (1). Bootstrap values, shown in boldface above the branch, are percentages derived from 500 samplings; branch lengths are shown below the branch. The sequences generated from our study were deposited in GenBank under accession nos. EF396941–EF396952.

Main Article

References
  1. Kuno  G, Chang  GJ. Biological transmission of arboviruses: reexamination of and new insights into components, mechanisms, and unique traits as well as their evolutionary trends. Clin Microbiol Rev. 2005;18:608–37. DOIPubMedGoogle Scholar
  2. Kuno  G, Chang  GJ, Tsuchiya  KR, Karabatsos  N, Cropp  CB. Phylogeny of the genus Flavivirus. J Virol. 1998;72:73–83.PubMedGoogle Scholar
  3. Monath  TP, Heinz  F. Flaviviruses. In: Knipe D, Howley P, editors. Fields virology. 4th ed. Philadelphia: Lippincott Williams and Wilkins; 1996. p. 1043–125.
  4. Laemmert  H, Hughes  T. The virus of Ilheus encephalitis: isolation, serological specificity and transmission. J Immunol. 1947;55:61–7.
  5. Srihongse  S, Johnson  CM. The isolation of Ilheus virus from a man in Panama. Am J Trop Med Hyg. 1967;16:516–8.PubMedGoogle Scholar
  6. Pinheiro  F, Travassos da Rosa  A. Ilheus fever. In: Beran G, Steel J, editors. CRC handbook of zoonoses. Boca Raton (FL): CRC Press; 1994. p. 210.
  7. Figueiredo  LT. The Brazilian flaviviruses. Microbes Infect. 2000;2:1643–9. DOIPubMedGoogle Scholar
  8. Nassar  ES, Coimbra  TL, Rocco  IM, Pereira  LE, Ferreira  IB, de Souza  LT, Human disease caused by an arbovirus closely related to Ilheus virus: report of five cases. Intervirology. 1997;40:247–52. DOIPubMedGoogle Scholar
  9. Prias-Landinez  E, Bernal-Cubides  C, Morales-Alarcon  A. Isolation of Ilheus virus from a man in Colombia. Am J Trop Med Hyg. 1968;17:112–4.PubMedGoogle Scholar
  10. Kumar  S, Tamura  K, Nei  M. MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform. 2004;5:150–63. DOIPubMedGoogle Scholar

Main Article

Page created: July 05, 2010
Page updated: July 05, 2010
Page reviewed: July 05, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external