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Volume 13, Number 7—July 2007
Research

Response to Emerging Infection Leading to Outbreak of Linezolid-Resistant Enterococci

Marion A. Kainer*Comments to Author , Rose A. Devasia*†, Timothy F. Jones*, Bryan P. Simmons‡, Kelley Melton‡, Susan Chow‡, Joyce Broyles‡, Kelly L. Moore*, Allen S. Craig*, and William Schaffner§
Author affiliations: *Tennessee Department of Health, Nashville, Tennessee, USA; †Centers for Disease Control and Prevention, Atlanta, Georgia, USA; ‡Methodist University Hospital, Memphis, Tennessee, USA; §Vanderbilt University School of Medicine, Nashville, Tennessee, USA;

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Figure 3

Pulsed-field gel electrophoresis of linezolid-resistant enterococci (LRE) isolates, hospital A, Tennessee. A) Digestion with ApaI. B) Digestion with SmaI. Isolates labeled A, B, C, and D refer to patients mentioned in the text. Blood, isolate from blood specimen culture; Rect, isolate from perirectal/rectal swab specimen culture; Env, environmental isolate; H9812, S. Braenderup H9812 strain (ATCC BAA-664) (27) used as size marker.

Figure 3. Pulsed-field gel electrophoresis of linezolid-resistant enterococci (LRE) isolates, hospital A, Tennessee. A) Digestion with ApaI. B) Digestion with SmaI. Isolates labeled A, B, C, and D refer to patients mentioned in the text. Blood, isolate from blood specimen culture; Rect, isolate from perirectal/rectal swab specimen culture; Env, environmental isolate; H9812, S. Braenderup H9812 strain (ATCC BAA-664) (27) used as size marker.

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References
  1. Bonten  MJ, Willems  R, Weinstein  RA. Vancomycin-resistant enterococci: why are they here, and where do they come from. Lancet Infect Dis. 2001;1:31425. DOIPubMedGoogle Scholar
  2. Delisle  S, Perl  T. Vancomycin-resistant enterococci: a road map on how to prevent the emergence and transmission of antimicrobial resistance. Chest. 2003;123(Suppl 5):504S13S. DOIPubMedGoogle Scholar
  3. Stosor  V, Peterson  L, Postelnick  M, Noskin  G. Enterococcus faecium bacteremia. Arch Intern Med. 1998;158:5227. DOIPubMedGoogle Scholar
  4. Pfizer, Inc. Linezolid (Zyvox) [package insert]. Peapack (NJ): Pharmacia & Upjohn Company, Division of Pfizer, Inc.; 2000.
  5. Food and Drug Administration. Warning letter to Pfizer: Re NDA 21–130; 21–131; 21–132 Zyvox (linezolid) injection, tablets and oral suspension MACMIS #13544, Dated July 20, 2005. Rockville (MD): US Department of Health and Human Services, FDA; 2005. [cited 2006 December 28].Available from http://www.fda.gov/cder/warn/2005/Zyvox_wl.pdf
  6. Meka  VG, Gold  HS. Antimicrobial resistance to linezolid. Clin Infect Dis. 2004;39:10105. DOIPubMedGoogle Scholar
  7. Sharpe  JN, Shively  EH, Polk  HC Jr. Clinical and economic outcomes of oral linezolid versus intravenous vancomycin in the treatment of MRSA-complicated, lower-extremity skin and soft-tissue infections caused by methicillin-resistant Staphylococcus aureus. Am J Surg. 2005;189:4258. DOIPubMedGoogle Scholar
  8. Wunderink  RG, Rello  J, Cammarata  SK, Croos-Dabrera  RV, Kollef  MH. Linezolid vs. vancomycin: analysis of two double-blind studies of patients with methicillin-resistant Staphylococcus aureus nosocomial pneumonia. Chest. 2003;124:178997. DOIPubMedGoogle Scholar
  9. Kollef  MH, Rello  J, Cammarata  SK, Croos-Dabrera  RV, Wunderink  RG. Clinical cure and survival in gram-positive ventilator-associated pneumonia: retrospective analysis of two double-blind studies comparing linezolid with vancomycin. Intensive Care Med. 2004;30:38894. DOIPubMedGoogle Scholar
  10. Bassetti  M, Farrel  PA, Callan  DA, Topal  JE, Dembry  LM. Emergence of linezolid-resistant Enterococcus faecium during treatment of enterococcal infections. Int J Antimicrob Agents. 2003;21:5934. DOIPubMedGoogle Scholar
  11. Klare  I, Konstabel  C, Mueller-Bertling  S, Werner  G, Strommenger  B, Kettlitz  C, Spread of ampicillin/vancomycin-resistant Enterococcus faecium of the epidemic-virulent clonal complex-17 carrying the genes esp and hyl in German hospitals. Eur J Clin Microbiol Infect Dis. 2005;24:81525. DOIPubMedGoogle Scholar
  12. Marra  AR, Major  Y, Edmond  MB. Central venous catheter colonization by linezolid-resistant, vancomycin-susceptible Enterococcus faecalis. J Clin Microbiol. 2006;44:19156. DOIPubMedGoogle Scholar
  13. Johnson  AP, Tysall  L, Stockdale  MV, Woodford  N, Kaufmann  ME, Warner  M, Emerging linezolid-resistant Enterococcus faecalis and Enterococcus faecium isolated from two Austrian patients in the same intensive care unit. Eur J Clin Microbiol Infect Dis. 2002;21:7514. DOIPubMedGoogle Scholar
  14. Bonora  MG, Ligozzi  M, Luzzani  A, Solbiati  M, Stepan  E, Fontana  R. Emergence of linezolid resistance in Enterococcus faecium not dependent on linezolid treatment. Eur J Clin Microbiol Infect Dis. 2006;25:1978. DOIPubMedGoogle Scholar
  15. Seedat  J, Zick  G, Klare  I, Konstabel  C, Weiler  N, Sahly  H. Rapid emergence of resistance to linezolid during linezolid therapy of an Enterococcus faecium infection. Antimicrob Agents Chemother. 2006;50:42179. DOIPubMedGoogle Scholar
  16. Auckland  C, Teare  L, Cooke  F. Linezolid-resistant enterococci: report of the first isolates in the United Kingdom. J Antimicrob Chemother. 2002;50:7436. DOIPubMedGoogle Scholar
  17. Gonzales  RD, Schreckenberger  PC, Graham  MB, Kelkar  S, DenBesten  K, Quinn  JP. Infections due to vancomycin-resistant Enterococcus faecium resistant to linezolid. Lancet. 2001;357:1179. DOIPubMedGoogle Scholar
  18. Herrero  IA, Issa  NC, Patel  R. Nosocomial spread of linezolid-resistant, vancomycin-resistant Enterococcus faecium. N Engl J Med. 2002;346:8679. DOIPubMedGoogle Scholar
  19. Bonora  MG, Solbiati  M, Stepan  E, Zorzi  A, Luzzani  A, Catania  MR, Emergence of linezolid resistance in the vancomycin-resistant Enterococcus faecium multilocus sequence typing C1 epidemic lineage. J Clin Microbiol. 2006;44:11535. DOIPubMedGoogle Scholar
  20. Liao  CH, Tseng  SP, Fang  CT, Teng  LJ, Hsueh  PR. First linezolid- and vancomycin-resistant Enterococcus faecium strain in Taiwan. J Antimicrob Chemother. 2005;55:5989. DOIPubMedGoogle Scholar
  21. Dobbs  TE, Patel  M, Waites  KB, Moser  SA, Stamm  AM, Hoesley  CJ. Nosocomial spread of Enterococcus faecium resistant to vancomycin and linezolid in a tertiary care medical center. J Clin Microbiol. 2006;44:336870. DOIPubMedGoogle Scholar
  22. Pai  MP, Rodvold  KA, Schreckenberger  PC, Gonzales  RD, Petrolatti  JM, Quinn  JP. Risk factors associated with the development of infection with linezolid- and vancomycin-resistant Enterococcus faecium. Clin Infect Dis. 2002;35:126972. DOIPubMedGoogle Scholar
  23. Buckingham  SC, McDougal  LK, Cathey  LD, Comeaux K, Craig AS, Fridkin SK, et al. Emergence of community-associated methicillin-resistant Staphylococcus aureus at a Memphis, Tennessee children’s hospital. Pediatr Infect Dis J. 2004;23:61924. DOIPubMedGoogle Scholar
  24. Naidu  SI, Donepudi  SK, Stocks  RMS, Buckingham  SC, Thompson  JW. Methicillin-resistant Staphylococcus aureus as a pathogen in deep neck abscesses: a pediatric case series. Int J Pediatr Otorhinolaryngol. 2005;69:136771. DOIPubMedGoogle Scholar
  25. Qi  C, Zheng  X, Obias  A, Scheetz  MH, Malczynski  M, Warren  JR. Comparison of testing methods for detection of decreased linezolid susceptibility due to G2576T mutation of the 23S rRNA gene in Enterococcus faecium and Enterococcus faecalis. J Clin Microbiol. 2006;44:1098100. DOIPubMedGoogle Scholar
  26. Graves  LM, Swaminathan  B. PulseNet standardized protocol for subtyping Listeria monocytogenes by macrorestriction and pulsed-field gel electrophoresis. Int J Food Microbiol. 2001;65:5562. DOIPubMedGoogle Scholar
  27. Hunter  SB, Vauterin  P, Lambert-Fair  MA, van Duyne  MS, Kubota  K, Graves  L, Establishment of a universal size standard strain for use with the PulseNet standardized pulsed-field gel electrophoresis protocols: converting the national databases to the new size standard. J Clin Microbiol. 2005;43:104550. DOIPubMedGoogle Scholar
  28. Tenover  FC, Arbeit  RD, Goering  RV, Mickelsen  PA, Murray  BE, Persing  DH, Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol. 1995;33:22339.PubMedGoogle Scholar
  29. Harris  AD, Samore  MH, Lipsitch  M, Kaye  KS, Perencevich  E, Carmeli  Y. Control-group selection important in studies of antimicrobial resistance: examples applied to Pseudomonas aeruginosa, enterococci, and Escherichia coli. Clin Infect Dis. 2002;34:155863. DOIPubMedGoogle Scholar
  30. Harris  AD, Karchmer  TB, Carmeli  Y, Samore  MH. Methodological principles of case-control studies that analyzed risk factors for antimicrobial resistance: a systematic review. Clin Infect Dis. 2001;32:105561. DOIPubMedGoogle Scholar
  31. Rahim  S, Pillai  S, Gold  H, Venkataraman  L, Inglima  K, Press  R. Linezolid-resistant, vancomycin-resistant Enterococcus faecium infection in patients without prior exposure to linezolid. Clin Infect Dis. 2003;36:e1468. DOIPubMedGoogle Scholar
  32. Muto  CA, Jernigan  JA, Ostrowsky  BE, Richet HM, Jarvis WR, Boyce JM, SHEA guideline for preventing nosocomial transmission of multidrug-resistant strains of Staphylococcus aureus and Enterococcus. Infect Control Hosp Epidemiol. 2003;24:36286. DOIPubMedGoogle Scholar
  33. Goldmann  DA, Weinstein  RA, Wenzel  RP, Tablan OC, Duma RJ, Gaynes RP, Strategies to prevent and control the emergence and spread of antimicrobial-resistant microorganisms in hospitals: a challenge to hospital leadership. JAMA. 1996;275:23440. DOIPubMedGoogle Scholar
  34. Siegel  JD, Rhinehart  E, Jackson  M. Chiarello L, Healthcare Infection Control Practices Advisory Committee. Management of multidrug-resistant organisms in healthcare settings, 2006. [cited 2007 May 3]. Available from http://www.cdc.gov/ncidod/dhqp/pdf/ar/mdroguideline2006.pdf

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Page created: June 21, 2010
Page updated: June 21, 2010
Page reviewed: June 21, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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