From July 2004 to March 2005, we studied 69 women with sporadic miscarriages (SM), 200 women who had suffered from recurrent miscarriages (RM), and 169 control women who had had uneventful pregnancies (Table 1). The RM group comprised women who had >3 miscarriages and who had attended the Recurrent Miscarriage Clinic of St Mary’s Hospital, London, the largest specialist referral center in Europe.

Immunofluorescence tests were performed (9); we used W. chondrophila strain ATCC VR-1470 as antigen and we screened sera at a 1:64 dilution with FluolineH (bioMerieux, Marcy l’Etoile, France). Antigen was isolated as described (10,11). Mice and rabbit anti-Waddlia antibodies were used as positive controls with a fluorescein-conjugated anti-mouse and anti-rabbit globulin. Sera that exhibited an Ig titer >64 were tested for IgG and IgM reactivity by using corresponding anti-human Ig fluorescein (FluolineG or FluolineM, bioMérieux) and serial 2-fold dilutions of serum. IgG and IgM positivity cut-offs were >1:64 and >1:32, as proposed for other chlamydia-like organisms. One hundred women had an anti-Waddlia IgG titer >64 (Table 1). Seroprevalence was higher for patients who had sporadic (31.9%) and recurrent (33%) miscarriages than that for women who had had uneventful pregnancies (7.1%, p<0.001 when comparing either SM or RM groups to controls). One woman had a positive IgM titer of 64 and an IgG titer of 512.

Figure 1

Figure 1. Western blot analyses. A) Molecular weight and frequency of IgG reactivity of Waddlia proteins, as determined by Western blots. B) and C) Four representative Western blot patterns of Waddlia IgG positive...

To confirm the specificity of immunofluorescence, we performed Western blot analyses on all Waddlia-positive sera samples. Western blot was performed (9), but Waddlia was used as the antigen. A polyclonal peroxidase-labeled anti-human IgG (Dako, Glostrup, Denmark) was used as a secondary antibody. The presence of anti-Waddlia IgG antibodies was confirmed by Western blot in 97 of the 100 positive samples by immunofluorescence (Table 1; Figure 1, panels B and C). By using Waddlia spp. hyperimmune mouse and rabbit sera and corresponding peroxidase-conjugated anti-mouse/rabbit sera, we obtained similar patterns of 61-, 55-, 53-, 45-, 41-, 38- and 30-kDa proteins (Figure 1, panels D and E). Antibody reactivity against the 55-, 53-, 45-, 41- and 38-kDa proteins disappeared after adsorption with 10⁸ Waddlia antigen for 48 hours, which demonstrated the specificity of the antibody response (Figure 1, panel F).

For further statistical analyses, only patients whose samples were positive for Waddlia spp. by immunofluorescence and confirmed by Western blot were considered seropositive for Waddlia spp. (n = 97). In all age groups, the rate of Waddlia seropositivity was higher in patients who had miscarried than in those who had not (data not shown). Moreover, most women with anti-Waddlia antibodies did not exhibit serologic reactivity against C. trachomatis (Table 2).

Figure 2

Figure 2. Multivariate analyses. A) Multivariate analysis adjusted for all variables listed in this figure and showing the independent association of age, positive Waddlia serologic results and positive Chlamydia trachomatis serologic results for...

In a multivariate logistic regression adjusted for age, ethnicity, contact with animals and C. trachomatis serostatus (Figure 2), miscarriage (SM/RM) remained strongly associated with Waddlia seropositivity (odds ratio [OR] 4.9, 95% confidence interval [CI] 2.5–9.4). In this model, miscarriage was also independently associated with age (OR 2.9, 95% CI 2.0–4.1) and C. trachomatis seropositivity (OR 2.3, 95% CI 1.2–4.5). Additional multivariate models confirmed the association between Waddlia IgG seropositivity and miscarriage, with ORs ranging from 4.9 to 6.2.

Noninfectious causes of miscarriage have also been recorded (Table 2). When studying only the 322 patients without known concurrent conditions, the strong correlation between Waddlia seropositivity and miscarriage was still present (68/195 [34.8%] of patients who had had a miscarriage vs. 10/127 [7.9%] of patients who had not miscarried had anti-Waddlia antibodies; p<0.001).

Waddlia seropositivity was associated with early miscarriage (p<0.001, Table 2). No difference in ethnicity was observed between those who were IgG positive for Waddlia spp. and those who were negative. Moreover, there was no difference in C. trachomatis, Chlamydophila pneumoniae, or Cp. psittaci seropositivity between women who had anti-Waddlia antibodies and those who did not, which suggests that Waddlia antibodies do not cross-react with Chlamydiaceae. Moreover, only 7 (1.6%) of 438 patients had a Parachlamydia IgG titer >64. With such a low Parachlamydia prevalence, cross-reactivity with this chlamydia-like organism is unlikely to explain the high Waddlia seroprevalence observed in the miscarriage groups.

Women who were IgG seropositive for Waddlia spp. were more likely to have had contact with animals. In a multivariate logistic regression model adjusted for age, ethnicity, and C. trachomatis serostatus, those who had had previous contact with animals were more likely to exhibit anti-Waddlia antibodies (OR 1.7, 95% CI 1.0–2.9, Figure 2). In this model, Waddlia IgG–positive serologic test results were also independently associated with age (OR 2.1, 95% CI 1.4–3.1).

This study demonstrates a strong association between the presence of W. chondrophila–specific IgG antibodies and early fetal loss. Cross-reactivity with other microorganisms seems an unlikely explanation for our results because W. chondrophila did not react with monoclonal or polyclonal antisera directed against Rickettsia, Coxiella, Wolbachia, Anaplasma, and Chlamydia spp (8,12). We did not detect any cross-reactivity of W. chondrophila with C. trachomatis, Cp. pneumoniae, and Cp. psittaci. Moreover, the molecular weights of Waddlial immunoreactive proteins obtained by Western blot are clearly different than those reported for C. trachomatis or Cp. pneumoniae (13).

With the exception of 1 patient who had IgM, only IgG antibody reactivity against W. chondrophila was observed. Because IgG antibodies may persist for years after an acute infection has resolved (3), the underlying mechanism of miscarriage due to W. chondrophila may involve reactivation of a latent asymptomatic waddlial infection, endometrial damage from a past waddlial infection, or an immune response to an epitope shared by a waddlial and fetal antigen, as proposed for C. trachomatis (3).

The association we found between contact with animals and positive serologic results for Waddlia spp. raises the zoonotic potential of this bacterium. This hypothesis is further supported by the range of hosts for Waddliaceae. Other modes of transmission are possible (e.g., contaminated water) because free-living amebae may serve as hosts for Waddlia spp (14). and are widespread in water networks (15). Waddlia spp. may also be transmitted through ingestion of contaminated cow milk. Finally, Waddlia spp. might represent a sexually transmitted disease.

Further investigations are urgently needed to define how Waddlia spp. infection may be acquired. To confirm the role of W. chondrophila in miscarriage, it will be important to isolate this intracellular bacterium from miscarriage products or confirm its presence in the placenta by immunohistochemistry or PCR. This may be difficult to achieve, if, as suggested for C. trachomatis (3), W. chondrophila causes miscarriage indirectly, e.g., through increased cytokine production or molecular mimicry with fetal antigens. To our knowledge, this work provides the first evidence that W. chondrophila may be implicated in human fetal death.