Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 14, Number 1—January 2008
Dispatch

Protochlamydia naegleriophila as Etiologic Agent of Pneumonia

Nicola Casson*, Rolf Michel†, Karl-Dieter Müller, John David Aubert*, and Gilbert Greub*Comments to Author 
Author affiliations: *University Hospital Center and University of Lausanne, Lausanne, Switzerland; †Central Institute of the Federal Armed Forces Medical Services, Koblenz, Germany; ‡Institut für Medizinische Mikrobiologie der Universität Essen, Essen, Germany;

Main Article

Table 1

Antibody titers and serologic differentiation index (SDI) obtained from reciprocal cross-reactions of mouse antiserum with different Chlamydia-like organisms, as determined by immunofluorescence* www.cdc.gov/EID/content/14/1/168-T1.htm

Strains Antigen titers (SDI)
Pr. naegleriophila Pr. amoebophila Simkania negevensis P. a. strain Seine Waddlia chondrophila Neoclamydia hartmannellae Criblamydia sequanensis R. crassificans
Pr. naegleriophila 4,096 (0) 256 (7) <4 (14) 512 (10) 4 (20) 16 (14) <4 (20) <4 (13)
Pr. amoebophila 32 (7) 256 (0) <32 (5) 1,024 (3) 64 (10) 32 (0) 128 (9) 64 (3)
S. negevensis 32 (14) 128 (5) 512 (0) 64 (12) 128 (12) <32 (12) 512 (8) 256 (3)
P. strain Seine 128 (10) 512 (3) 32 (12) 16,384 (0) 64 (19) 64 (12) <32 (19) <32 (12)
W. chondrophila 32 (20) 128 (10) 32 (12) <32 (19) 32,768 (0) <32 (18) 512 (13) 128 (10)
N. hartmannellae 32 (14) 128 (0) <32 (12) 128 (12) <32 (18) 2,048 (0) <32 (18) <32 (11)
C. sequanensis 32 (20) 128 (9) 128 (8) 64 (19) 256 (13) <32 (18) 32,768 (0) 128 (8)
R. crassificans 32 (13) 128 (3) 64 (3) 64 (12) 64 (10) <32 (11) 256 (8) 256 (0)

*Pr., Protochlamydia; P. a., Parachlamydia acanthamoebae; R., Rhabdochlamydia. Titers highlighted in gray, previously published in (9), are provided here because these data were used to calculate SDI between Pr. naegleriophila strain KNic and the other Chlamydia-related bacteria.

Main Article

References
  1. Michel  R, Muller  KD, Hauröder  B, Zöller  L. A coccoid bacterial parasite of Naegleria sp. (Schizopyrenida: Vahlkampfiidae) inhibits cyst formation of its host but not transformation to the flagellate stage. Acta Protozool. 2000;39:199–207.
  2. Greub  G, Mege  JL, Raoult  D. Parachlamydia acanthamebae enters and multiplies within human macrophages and induces their apoptosis. Infect Immun. 2003;71:5979–85. DOIPubMedGoogle Scholar
  3. Greub  G, Raoult  D. Microorganisms resistant to free-living amoebae. Clin Microbiol Rev. 2004;17:413–33. DOIPubMedGoogle Scholar
  4. Greub  G, Raoult  D. Parachlamydiaceae: potential emerging pathogens. Emerg Infect Dis. 2002;8:625–30.PubMedGoogle Scholar
  5. Greub  G, Berger  P, Papazian  L, Raoult  D. Parachlamydiaceae as rare agents of pneumonia. Emerg Infect Dis. 2003;9:755–6.PubMedGoogle Scholar
  6. Greub  G, Boyadjiev  I, La Scola  B, Raoult  D, Martin  C. Serological hint suggesting that Parachlamydiaceae are agents of pneumonia in polytraumatized intensive care patients. Ann N Y Acad Sci. 2003;990:311–9.PubMedGoogle Scholar
  7. Casson  N, Medico  N, Bille  J, Greub  G. Parachlamydia acanthamoebae enters and multiplies within pneumocytes and lung fibroblasts. Microbes Infect. 2006;8:1294–300. DOIPubMedGoogle Scholar
  8. Greub  G, Raoult  D. Crescent bodies of Parachlamydia acanthamoebae and its life cycle within Acanthamoeba polyphaga: an electron micrograph study. Appl Environ Microbiol. 2002;68:3076–84. DOIPubMedGoogle Scholar
  9. Casson  N, Entenza  JM, Greub  G. Serological cross-reactivity between different Chlamydia-like organisms. J Clin Microbiol. 2007;45:234–6. DOIPubMedGoogle Scholar
  10. Fang  R, Raoult  D. Antigenic classification of Rickettsia felis by using monoclonal and polyclonal antibodies. Clin Diagn Lab Immunol. 2003;10:221–8. DOIPubMedGoogle Scholar
  11. Thomas  V, Casson  N, Greub  G. Criblamydia sequanensis, a new intracellular Chlamydiales isolated from Seine river water using amoeba coculture. Environ Microbiol. 2006;8:2125–35. DOIPubMedGoogle Scholar
  12. Kumar  S, Tamura  K, Nei  M. MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform. 2004;5:150–63. DOIPubMedGoogle Scholar
  13. Everett  KD, Bush  RM, Andersen  AA. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int J Syst Bacteriol. 1999;49:415–40.PubMedGoogle Scholar
  14. Jaton  K, Bille  J, Greub  G. A novel real-time PCR to detect Chlamydia trachomatis in first-void urine or genital swabs. J Med Microbiol. 2006;55:1667–74. DOIPubMedGoogle Scholar
  15. Welti  M, Jaton  K, Altwegg  M, Sahli  R, Wenger  A, Bille  J. Development of a multiplex real-time quantitative PCR assay to detect Chlamydia pneumoniae, Legionella pneumophila and Mycoplasma pneumoniae in respiratory tract secretions. Diagn Microbiol Infect Dis. 2003;45:85–95. DOIPubMedGoogle Scholar

Main Article

Page created: July 07, 2010
Page updated: July 07, 2010
Page reviewed: July 07, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external