Volume 14, Number 12—December 2008
Letter
Human Case of Rickettsia felis Infection, Taiwan
Kun-Hsien Tsai, Hsiu-Ying Lu, Jih-Jin Tsai, Sheng-Kai Yu, Jyh-Hsiung Huang, and Pei-Yun Shu
Table
Primer sequences for the SYBR-based real-time PCR assay*
| Primer | Genomic region | Sequence (5′ → 3′) | Amplicon size, bp |
|---|---|---|---|
| RR-F8 | 17-kDa (Rickettsia spp.) | GGC GGY GCA TTA CTT GGT TCT CAA TTC GC | 304 |
| RR-R12 | GTT TTC CSC CTA TTA CAA CTG TTT GAG T | ||
| RR-F1 | groEL gene (Rickettsia spp.) | AAA ATG GTT GCT GAG CTT GAA AAT CCT TT | 191 |
| RR-R2 | ACT TTC AAA CCA CCA CGT AAT CTA TTG AC | ||
| RR-F22 | ompB gene (Rickettsia spp.) | ATG GTR TAT GGG CWA AAC CTT TCT ATA | 330 |
| RR-R25 | TAG MMT CGA AGA AGT AAC GCT GAC TTT | ||
| RST-14F | 56-kDa gene (Orientia tsutsugamushi) | CCA TTT GGT GGT ACA TTA GCT GCA GGT | 233 |
| RST-6R | TCA CGA TCA GCT ATA CTT ATA GGC A | ||
| OMP3 | com-1 (Coxiella burnetii) | GAA GCG CAA CAA GAA GAA CAC | 438 |
| OMP4 | TTG GAA GTT ATC ACG CAG TTG |
*groEL, 60-kDa heat-shock protein; ompB, outer membrane protein B; com-1, 27-kDa outer membrane protein.
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