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Volume 14, Number 12—December 2008

Transmission of Atypical Bovine Prions to Mice Transgenic for Human Prion Protein

Vincent BéringueComments to Author , Laëtitia Herzog, Fabienne Reine, Annick Le Dur, Cristina Casalone, Jean-Luc Vilotte, and Hubert Laude
Author affiliations: Institut National de la Recherche Agronomique, Jouy-en-Josas, France (V. Béringue, L. Herzog, F. Reine, A. Le Dur, J.-L. Vilotte, H. Laude); Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Turin, Italy (C. Casalone)

Main Article


Transmission of classical and atypical BSE isolates to transgenic mice expressing human prion protein (Met129)*

Isolate Origin
(identification no.) 1st passage
2nd passage
3rd passage
Total no. affected† Mean survival time‡ Total no. affected† Mean survival time‡ Total no. affected† Mean survival time‡
BSE France (3) 1/6§ 872 6/7 568 ± 65 8/8 523 ± 22
France (3) 2/6 627, 842
Germany¶ 1/4§ 802 6/6 677 ± 54 8/8 555 ± 24
Italy (128204) 0/5 606–775
Belgium 0/4 696–829
L-type Italy (1088) 9/9 607 ± 23 11/11 653 ± 13 NA
France (7) 7/7 574 ± 35 0/8# >450
France (10) 8/8 703 ± 19
France (11) 9/9 647 ± 26
H-type France (1) 0/6 376–721 0/7 350–850 NA
France (2) 0/6 313–626 0/8 302–755 NA
France (5) 0/10 355–838 NA

*BSE, bovine spongiform encephalopathy; NA, not available (experiments still ongoing).
†Mice with neurologic signs and positive for protease-resistant prion protein by Western blotting.
‡Days ± SE of the mean. For mice with negative results, only the range of survival time is given.
§First passage performed on hemizygous mice. Note that the primary transmission of France (3) isolate was performed on both hemizygous and homozygous mice.
¶One passage on transgenic mice expressing bovine prion protein (7).
#Ongoing experiment.

Main Article

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