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Volume 14, Number 4—April 2008

Dispatch

Dengue Virus Type 4, Manaus, Brazil

Regina Maria Pinto de Figueiredo*1Comments to Author , Felipe Gomes Naveca†1, Michele de Souza Bastos*1, Miriam do Nascimento Melo*, Suziane de Souza Viana‡, Maria Paula Gomes Mourão*, Cristóvão Alves Costa‡, and Izeni Pires Farias§

Author affiliations: *Fundação de Medicina Tropical do Amazonas, Manaus, Brazil; †Fundação Alfredo da Matta, Manaus, Brazil; ‡Instituto Nacional de Pesquisas da Amazônia, Manaus, Brazil; §Universidade Federal do Amazonas, Manaus, Brazil

Main Article

Table

Results of different methods used to confirm dengue virus type 4 (DENV-4) infection, Manaus, Amazonas, Brazil*

Isolate IgM antibody capture ELISA Virus culture†
 M-PCR
 S-PCR
 BLAST
D1 D2 D3 D4 D1 D2 D3 D4 D1 D2 D3 D4
AM750 – – – + + – – + + – – + + DENV-3/ DENV-4‡
AM1041 + – – – + – – – + – – – + NS
AM1619 + – – – + – – – + – – – + DENV-4

*IgM, immunoglobulin M; M-PCR, multiplex typing PCR described by Lanciotti et al. (10); S-PCR, single serotype-specific primer PCR; D, dengue serotype; BLAST, basic local alignment search tool; NS, not sequenced.
†Viral antigens detected by immunofluorescence with type-specific monoclonal antibodies.
‡Isolate AM750 is from a person with a co-infection; different clones were sequenced.

Main Article

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1These authors contributed equally to this work.

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