Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 14, Number 4—April 2008
Dispatch

Dengue Virus Type 4, Manaus, Brazil

Regina Maria Pinto de Figueiredo*1Comments to Author , Felipe Gomes Naveca†1, Michele de Souza Bastos*1, Miriam do Nascimento Melo*, Suziane de Souza Viana‡, Maria Paula Gomes Mourão*, Cristóvão Alves Costa‡, and Izeni Pires Farias§

Author affiliations: *Fundação de Medicina Tropical do Amazonas, Manaus, Brazil; †Fundação Alfredo da Matta, Manaus, Brazil; ‡Instituto Nacional de Pesquisas da Amazônia, Manaus, Brazil; §Universidade Federal do Amazonas, Manaus, Brazil

Main Article

Table

Results of different methods used to confirm dengue virus type 4 (DENV-4) infection, Manaus, Amazonas, Brazil*

Isolate IgM antibody capture ELISA Virus culture†
 M-PCR
 S-PCR
 BLAST
D1 D2 D3 D4 D1 D2 D3 D4 D1 D2 D3 D4
AM750 – – – + + – – + + – – + + DENV-3/ DENV-4‡
AM1041 + – – – + – – – + – – – + NS
AM1619 + – – – + – – – + – – – + DENV-4

*IgM, immunoglobulin M; M-PCR, multiplex typing PCR described by Lanciotti et al. (10); S-PCR, single serotype-specific primer PCR; D, dengue serotype; BLAST, basic local alignment search tool; NS, not sequenced.
†Viral antigens detected by immunofluorescence with type-specific monoclonal antibodies.
‡Isolate AM750 is from a person with a co-infection; different clones were sequenced.

Main Article

References
  1. Gubler  DJ. Dengue and dengue haemorrhagic fever. Clin Microbiol Rev. 1998;11:480–96.PubMedGoogle Scholar
  2. Gubler  DJ. Epidemic dengue/dengue hemorrhagic fever as a public health, social and economic problem in the 21st century. Trends Microbiol. 2002;10:100–3. DOIPubMedGoogle Scholar
  3. World Health Organization. Dengue and dengue haemorrhagic fever. 2002 [cited 2008 Jan 9]. Available from http://www.who.int/mediacentre/factsheets/fs117/en
  4. Ministério da Saúde. Informe epidemiológico do SUS. 2000. Brasília (Brazil): Ministério da Saúde Press; 2000.
  5. Figueiredo  RM, Thatcher  BD, Lima  ML, Almeida  TC, Alecrim  WD, Guerra  MV. Exanthematous diseases and the first epidemic of dengue to occur in Manaus, Amazonas, State, Brazil, during 1998–1999 [in Portuguese]. Rev Soc Bras Med Trop. 2004;37:476–9. DOIPubMedGoogle Scholar
  6. Figueiredo  RM, Bastos  MS, Lima  ML, Almeida  TC, Alecrim  WD. Dinâmica da sorologia e isolamento viral na epidemia de dengue em Manaus (1998–2001). In: Abstracts of the 38th Congress of Brazilian Society of Tropical Medicine. Foz do Iguaçu, Paraná, Brazil; 2002 Feb 24–28. Brazilian Society of Tropical Medicine; 2002. p. 94.
  7. Figueiredo  RM, Silva  MN, Almeida  TC, Lopes  HC, Bastos  MS. Diagnóstico virológico de dengue em pacientes com quadro febril não diferenciado. In: Abstracts of the 39th Congress of Brazilian Society of Tropical Medicine. Belém, Pará, Brazil; 2003 Mar 11–15. Brazilian Society of Tropical Medicine; 2003. p. 485–6.
  8. Igarashi  A. Isolation of a Singh’s Aedes albopictus cell clone sensitive to dengue and chikungunya viruses. J Gen Virol. 1978;40:531–44.PubMedGoogle Scholar
  9. Kuno  G, Gomez  I, Gubler  DJ. Detecting artificial antidengue IgM immune complexes using an enzyme-linked immunosorbent assay. Am J Trop Med Hyg. 1987;36:153–9.PubMedGoogle Scholar
  10. Lanciotti  RS, Calisher  CH, Gubler  DJ, Chang  GJ, Vorndam  AV. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol. 1992;30:545–51.PubMedGoogle Scholar
  11. Ocazionez  RE, Cortes  FM, Villar  LA, Gomez  SY. Temporal distribution of dengue virus serotypes in Colombian endemic area and dengue incidence. Reintroduction of dengue-3 associated to mild febrile illness and primary infection. Mem Inst Oswaldo Cruz. 2006;101:725–31. DOIPubMedGoogle Scholar
  12. Lorono Pino  MA, Farfan Ale  JA, Rosado Paredes  EP, Kuno  G, Gubler  DJ. Epidemic dengue 4 in the Yucatan, Mexico, 1984. Rev Inst Med Trop Sao Paulo. 1993;35:449–55. DOIPubMedGoogle Scholar
  13. Bravo  JR, Guzmãn  MG, Kourí  GP. Why dengue haemorrhagic fever in Cuba? I. Individual risk factors for dengue haemorrhagic fever/dengue shock syndrome (DHF/DSS). Trans R Soc Trop Med Hyg. 1987;81:816–20. DOIPubMedGoogle Scholar
  14. Osanai  CH. A epidemia de Dengue em Boa Vista, território Federal de Roraima, 1981−1982 [dissertation]. Rio de Janeiro, Brazil: Escola Nacional de Saúde Pública; 1984.
  15. Lanciotti  RS, Gubler  DJ, Trent  DW. Molecular evolution and phylogeny of dengue-4 viruses. J Gen Virol. 1997;78:2279–86.PubMedGoogle Scholar

Main Article

1These authors contributed equally to this work.

Page created: July 14, 2010
Page updated: July 14, 2010
Page reviewed: July 14, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external