Volume 14, Number 7—July 2008
Research
Spotted Fever Group Rickettsiae in Ticks, Morocco
Table
Detection and identification of spotted fever group Rickettsia spp. from ticks collected in Morocco, by PCR and DNA sequencing
Tick species (no. specimens tested) | Host | Rickettsial gene targeted/no. ticks positive by PCR/no. examined* | Identification by gene sequence (no. identified/no. tested) | GenBank accession no. |
---|---|---|---|---|
Dermacentor marginatus (11) | Vegetation | gltA/6/11 | R. slovaca (5/6), R. raoultii (1/6) | U59725, DQ365803 |
ompA/6/6 |
R. slovaca (5/6),
R. raoultii (1/6) |
U43808, DQ365799 |
||
Hyalomma marginatum marginatum (35) | Domestic animals | gltA/3/35 | R. aeschlimannii | U59722 |
ompA/3/3 |
R. aeschlimannii |
DQ379982 |
||
Haemaphysalis sulctata (79) | Domestic animals | gltA/61/79 | “Rickettsia endosymbiont of Ha. Sulctata” (“R. kastelanii”) | DQ081187 |
ompA/0/61 |
– |
– |
||
Ha. punctata (6) | Domestic animals | gltA/3/6 | “Rickettsia endosymbiont of Ha. Sulctata” (“R. kastelanii”) | DQ081187 |
ompA/0/3 |
||||
Haemaphysalis sp. (18) | Vegetation | gltA/14/18 | “Rickettsia endosymbiont of Ha. Sulctata” (“R. kastelanii”) | DQ081187 |
ompA/0/14 |
– |
– |
||
Ixodes ricinus (14) | Vegetation | gltA/9/14 | R. monacensis (5/9), R. helvetica (4/9) | AF140706, U59723 |
ompA/5/9 |
R. monacensis (5/9) |
AJ427885 |
||
Rhipicephalus sanguineus (106) | Domestic animals | gltA/5/106 | R. massiliae | U59720 |
ompA/5/5 |
R. massiliae |
U43792 |
||
Rh. bursa (76) |
Domestic animals |
gltA/0/76 |
– |
– |
Rh. turanicus (25) | Domestic animals | gltA/0/25 | – | – |
*gltA, citrate synthase A; ompA, outer membrane protein A. Only ticks positive for the gltA gene were tested for the ompA gene.