We used mass spectrometry (MS)–based proteomics, as described by Breci et al. and Koller et al. (11,12). Individual ticks or pools were pulverized after freezing in liquid nitrogen. Total proteins were precipitated in 95% ethanol at –20°C and recovered by centrifugation. Proteins of individual ticks were reduced with 100 mmol/L dithiothreitol, alkylated with 50 mmol/L iodoacetamide, digested with trypsin at a final concentration of 0.01 μg/μL, and filtered through a C18 cartridge before being subjected to liquid chromatography (LC) with a 5%–50% acetonitrile gradient in 0.1% formic acid, followed by tandem MS (LC-MS/MS; LTQ ThermoElectron, San Jose, CA, USA). Proteins of pooled ticks were separated by 1-dimensional sodium dodecyl sulfate–polyacrylamide gel electrophoresis, and gel slices were digested in situ with trypsin before using LC-MS/MS. Upwards of ≈7,000 spectra were submitted to a protein identification algorithm for each pooled sample. Sizes of sequenced peptides ranged from 5 aa to 34 aa. The SEQUEST search algorithm (http://fields.scripps.edu/sequest) with data-filtering criteria was used to identify sequence matches of output against databases of rabbit, sheep, deer, goat, mouse (Mus musculus), and tick proteins.

We studied I. scapularis and the lone-star tick, Amblyomma americanum, which is a vector of human monocytic ehrlichiosis in the United States. Ticks were provided by the tick-rearing facility of the Department of Entomology and Plant Pathology of Oklahoma State University (Stillwater, OK, USA). We examined pools of 15 A. americanum nymphs that had fed on sheep or rabbits as larvae and were 3 months postmolt. Predominant vertebrate peptides in all pools were α- and β-globin chains of hemoglobin and immunoglobulins. Sequences of these proteins corresponded to the source of the blood for the ticks. Other mammalian proteins detected in pools from ticks fed on sheep or rabbits were histone H3, histone H2, mitochondrial malate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, mitochondrial ATP synthase, interferon regulatory factor, α tubulin, β tubulin, and transferrin.

Figure

Figure. Tandem mass spectra of 2 peptides from sheep hemoglobin β-subunit identified in a nymph of an Amblyomma americanum tick. A) Singly protonated AAVTGFWGK. B) Doubly protonated VKVDEVGAEALGR. The peaks are labeled...

We then studied individual ticks that had fed as larvae on mice (I. scapularis), rabbits (A. americanum and I. scapularis), or sheep (A. americanum) and examined flat nymphs of A. americanum at 7 months postmolt and I. scapularis at 3–11 months postmolt. We also examined I. scapularis adults (2 males and 1 female) that had fed as larvae and nymphs on mice and were 3–5 months postmolt. Concentrations of extracted proteins from individual ticks were 50–70 μg/tick. The Figure shows representative LC-MS/MS spectra of an A. americanum nymph that had fed on a sheep as a larva. Panel A shows the tandem mass spectrum for the singly charged peptide AAVTGFWGK, corresponding to residues 8–16 of sheep hemoglobin β-subunit (P02075). Panel B shows the tandem mass spectrum for doubly charged VKVDEVGAEALGR, corresponding to residues 17–29 of the same protein. These 2 peptides cover 15.2% of the protein sequence and differ from the orthologous sequence of rabbit (P02099) at 8 of 22 positions.

The Table summarizes results of all individual tick analyses. There was no correlation between number of proteins detected and postmolt period. Although some proteins, such as immunoglobulin and histone H3, were detected in both species, other proteins distinguished between A. americanum and I. scapularis. Globin chains were more commonly found in A. americanum than in I. scapularis nymphs. Cytochrome c-type heme lyase, which binds heme moieties and is transported from the cytoplasm to mitochondria of eukaryotes, was present in all samples of I. scapularis but not in A. americanum (2-sided p<0.001, by likelihood ratio). Peptides detected included those specific for the host animal for the blood meal.

Digestion of a blood meal in ticks differs from what generally occurs in hematophagous insects. In ticks, digestion takes place gradually within cells of the intestinal tract after endocytosis, rather than by intraluminal enzymatic breakdown of blood cells and plasma components, as in insects (13). The combination of slow assimilation and uptake of some host proteins into hemolymph may explain persistence of host blood proteins for months after feeding and molting. Immunoglobulins in hemolymph have been demonstrated, but demonstration of several other proteins, including globin chains, histones, and mitochondrial enzymes, indicates that host protein persistence is not limited to 1 type of molecule.

The success of our study depended on access to a large database of protein sequences for sheep, rabbits, and laboratory mice. For most host species for these ticks in nature, such as the white-footed mouse (Peromyscus leucopus), protein databases currently are much less extensive. Thus, a priority before application of this approach is MS-based sequencing of highly prevalent proteins from the blood of P. leucopus and other host species. Although LC-MS/MS of individual ticks is feasible and highly sensitive, its cost confines it to exploratory studies. High-throughput analysis of hundreds or thousands of specimens will likely require species-specific assays that use antibodies or aptamers for detection and identification of selected proteins.

Understanding contributions of different vertebrate hosts to pathogen maintenance is a prerequisite for effective monitoring, modeling, and disease prevention efforts that focus on natural reservoirs. Unfortunately, this level of understanding has not been broadly achieved. A major impediment to success in this area for most tick-borne zoonoses has been the absence of reliable and reproducible methods for identification of the vertebrate source of the infection for the tick vector by characterizing residual blood components. Our study shows a way to achieve this goal. Similar data on uninfected ticks would establish the denominator for prevalence studies and indicate the relative competence of different host species.