Novel Human Rotavirus Genotype G5P[7] from Child with Diarrhea, Cameroon

Mathew D. Esona¹

, Annelise Geyer¹, Krisztian Banyai, Nicola Page¹, Maryam Aminu¹, George E. Armah¹, Jennifer Hull, Duncan A. Steele¹, Roger I. Glass, and Jon R. Gentsch

Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (M.D. Esona, J. Hull, J. Gentsch); University of Limpopo, Pretoria, South Africa (A. Geyer); Association of Public Health Laboratories, Washington, DC, USA (K. Banyai); National Institute for Communicable Diseases, Johannesburg, South Africa (N. Page); Ahmadu Bello University, Zaria, Nigeria (M. Aminu); Noguchi Memorial Research Institute, Accra, Ghana (G.E. Armah); Program for Appropriate Technology in Health, Seattle, Washington, USA (D.A. Steele); National Institutes of Health, Bethesda, Maryland, USA (R.I. Glass);

Main Article

Table 1

Primers used for amplification and sequencing of rotavirus genes

Primer*	Sequence (5′ → 3′)†	Gene‡	Nucleotide position, strand	Amplicon size, bp	Strain	Reference
MDEVP1F	AAT CAC AAT CTG CAG TTC AAA	VP1	68–89, +	337	Ku	This article
MDEVP1R	AAT GAA TCA GTG TAT TCT TCG	VP1	405–384, –	337	Ku	This article
MDEVP2F	CTG ACA AAG TGC TAT CAC A	VP2	156–175, +	300	Ku	This article
MDEVP2R	AGG TAA TTG TCT TGG TTC	VP2	456–438, –	300	Ku	This article
MDEVP3F	TTG CTA GAT TGT CAA ATC GTG	VP3	597–618, +	327	Ku	This article
MDEVP3R	AAT AAG ATG GAG CTG AAC C	VP3	924–905, –	327	Ku	This article
MDENSP1F	GAG ACC RTC AAC TCC TAC YAA	NSP1	120–141, +	344	Wa	This article
MDENSP1R	ATT GTA AYG TTA TTG GCA T	NSP1	464–445, –	344	Wa	This article
MDENSP2F	GCT TGC TTT TGT TAT CCT	NSP2	58–76, +	327	Ku	This article
MDENSP2R	ATT TTC CAA ATG TCT AAC AG	NSP2	385–365, –	327	Ku	This article
MDENSP3F	GCC ACT TCA ACA TTA GAA	NSP3	101–119, +	303	Ku	This article
MDENSP3R	TAC ACT AAA ACA AGC ATT AAG	NSP3	404–383, –	303	Ku	This article
MDENSP5F	AGC GCT ACA GTG ATG TCT CT	NSP5	10–29, +	337	Ku	This article
MDENSP5R	CCA TTT GAT CGC ACC CA	NSP5	347–330, –	337	Ku	This article
JRG30	GGC TTT TAA AAG TTC TGT T	NSP4	1–19, +	737	Wa	This article
JRG31	ACC ATT CCT TCC ATT AAC	NSP4	738–721, –	737	Wa	This article
Con3	TGG CTT CGC TCA TTT ATA GAC A	VP4	11–32, +	876	Ku	(5)
Con2	ATT TCG GAC CAT TTA TAA CC	VP4	887–868, –	876	Ku	(5)
9con1-L	TA GCT CCT TTT AAT GTA TGG TAT	VP7	37–59, +	896	Wa	Modified from (6)
VP7-Rdeg	GAC GGV GCR ACT ACA TGG T	VP7	933–914, –	896	Wa	Modified from (7)
VP6-F	GAC GGV GCR ACT ACA TGG T	VP6	747–766, +	379		(8)
VP6-R	GTC CAA TTC ATN CCT GGT G	VP6	1126–1106, –	379		(8)

*F, forward; R, reverse.
†R, A or G; Y, C or T; V, A, C, or G; N, A, C, G, or T.
‡VP, structural protein; NSP, nonstructural protein.

Main Article

¹Member of the African Rotavirus Network.

The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.

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Page created: December 06, 2010
Page last updated: December 06, 2010
Page last reviewed: December 06, 2010
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