Volume 15, Number 10—October 2009
Research
Discriminatory Ability of Hypervariable Variable Number Tandem Repeat Loci in Population-based Analysis of Mycobacterium tuberculosis Strains, London, UK
Table 2
Number of DNA extracts (from n = 16) for which peaks were detected by different conditions for capillary electrophoresis of Mycobaterium tuberculosis after amplifying the loci with different polymerases, London, UK, 2005–2006*
Locus | Method† | Bioline polymerases‡ |
QIAGEN polymerases‡ |
|||
---|---|---|---|---|---|---|
BIOTAQ | Diamond | HotStartTaq | HotStartTaq Plus | |||
MIRU 26 | 1 | 16 | 16 | 16 (1) | 16 (1) | |
2 | 15 | 16 | 16 (1) | 16 (1) | ||
3 |
16 |
16 |
16 |
16 |
||
ETR-B | 1 | 16 | 16 | 16 (1) | 16 (1) | |
2 | 15 | 16 | 16 (1) | 16 (1) | ||
3 |
16 |
16 |
16 (2) |
16 (2) |
||
VNTR 1982 | 1 | 8 | 13 | 14 | 14 | |
2 | 9 | 13 | 12 | 14 | ||
3 |
6 |
11 |
12 |
14 |
||
VNTR 3232 | 1 | 11 | 15 | 13 | 14 | |
2 | 10 | 15 | 14 | 14 | ||
3 | 11 (3) | 15 (7) | 13 (6) | 13 (4) |
*MIRU, mycobacterial interspersed repetitive unit; ETR, exact tandem repeat; VNTR, variable number tandem repeats.
†Refer to Table 1.
‡Numbers in parentheses represent number of extracts whose calculated number of repeats were higher and lower than the expected value on the basis of that produced by the standard procedure (method 1).