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Volume 15, Number 3—March 2009

Merkel Cell Polyomavirus DNA in Respiratory Specimens from Children and Adults

Seweryn BialasiewiczComments to Author , Stephen B. Lambert, David M. Whiley, Michael D. Nissen, and Theo P. Sloots
Author affiliations: Sir Albert Sakzewski Virus Research Centre, Brisbane, Queensland, Australia (S. Bialasiewicz, S.B. Lambert, D.M. Whiley, M.D. Nissen, T.P. Sloots); University of Queensland Clinical Medical Virology Center, Brisbane (S. Bialasiewicz, S.B. Lambert, D.M. Whiley, M.D. Nissen, T.P. Sloots); Pathology Queensland; Brisbane (M.D. Nissen, T.P. Sloots)

Main Article

Table 2

Nucleotide variation in LT3 and VP1 sequences from 3 MCPyV strains detected in respiratory secretions from patients, Queensland, Australia, 2993*†

Isolate name Nucleotide position and change
843 3825 3875 3909 4022
MCC350‡ (EU375803) G T C C T
MCC339‡ (EU375804) C C G T A
MCV-B1 (LT3: FJ009185, VP1: FJ009188) C C
MCV-B2 (LT3: FJ009186, VP1: FJ009189) C C G A
MCV-B3 (LT3: FJ009187, VP1: FJ009190) C C

*LT3, partial large T antigen; VP1, major capsid protein; MCPyV, Merkel cell polyomavirus.
†Nucleotide changes are in relation to the prototype strain (boldface). Dashes indicate homology with MCC350. Nucleotide positions are given in a 5′ → 3′ early to late orientation, respective to alignment with MCC350. GenBank accession numbers are shown in parentheses.
‡Original MCPyV strain sequences described by Feng et al. (3).

Main Article

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