Piyada Linsuwanon, Sunchai Payungporn, Rujipat Samransamruajkit, Apiradee Theamboonlers, and Yong Poovorawan
Figure. Phylogenetic analysis of nucleotide sequences of the virus capsid protein (VP4) region of 5 human rhinovirus (HRV) strains (shown in boldface) isolated from 289 nasopharyngeal aspirate specimens, including those of 2 infants with refractory wheezing (C1 and C2), on the basis of amplification of VP4/2 by seminested reverse transcription–PCR. The tree was constructed by using the neighbor-joining method and Kimura’s 2-parameter distance with bootstrap replicated from 1,000 trees by using MEGA 4.0 (www.megasoftware.net). Scale bar indicates number of nucleotide substitutions per site. Human enterovirus (HEV) was used as an outgroup for comparison.