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Volume 15, Number 8—August 2009

Research

Transgenic Mice Expressing Porcine Prion Protein Resistant to Classical Scrapie but Susceptible to Sheep Bovine Spongiform Encephalopathy and Atypical Scrapie

Juan-Carlos Espinosa1, María-Eugenia Herva1, Olivier Andréoletti, Danielle Padilla, Caroline Lacroux, Hervé Cassard, Isabelle Lantier, Joaquin Castilla, and Juan-María TorresComments to Author 
Author affiliations: Centro de Investigación en Sanidad Animal, Madrid, Spain (J.-C. Espinosa, M.-E. Herva, D. Padilla, J. Castilla, J.-M. Torres); École Nationale Vétérinaire de Toulouse, Toulouse, France (O. Andréoletti, C. Lacroux, H. Cassard); Centre Institut National de la Recherche Agronomique de Tours, Nouzilly, France (I. Lantier)

Main Article

Table

Transmission of cattle BSE, sheep BSE, and sheep scrapie isolates in transgenic mice expressing porcine prion protein*

TSE isolate Genotype PrPres, kDa First passage
 Second passage
 Third passage
Survival time, dpi % (Attack rate) Survival time, dpi % (Attack rate) Survival time, dpi % (Attack rate)
Cattle-BSE1 – 19 >650 0 (0/12)† 269 ± 3 100 (10/10) 204 ± 12 100 (9/9)
Cattle-BSE2 – 19 498 ± 9 17 (2/12)† 198 ± 6 100 (15/15) 193 ± 17 100 (6/6)
Cattle-BSE0 – 19 >650 19 (3/16)† 197 ± 4 100 (12/12) 190 ± 10 100 (7/7)
Sheep-BSE0 ARQ/ARQ 19 458 ± 11 100 (15/15) 162 ± 4 100 (13/13) 166 ± 7 100 (7/7)
SC-662/97 ARQ/ARQ 21 >650 0 (0/10) >650 0 (0/12) ND ND
SC-UCD/99 ARQ/ARQ 21 >650 0 (0/12) >650 0 (0/9) ND ND
SC-Langlade ARQ/ARQ 21 >650 0 (0/12) >650 0 (0/12) ND ND
SC-PS13 ARQ/ARQ 20 >650 0 (0/12) >650 0 (0/12) ND ND
SC-JR01 VRQ/VRQ 21 >650 0 (0/12) >650 0 (0/12) ND ND
SC-PS83 ARR/ARR 21 >650 0 (0/12) >650 0 (0/12) ND ND
SC-PS48 VRQ/VRQ 19 >650 0 (0/9) >650 0 (0/10) ND ND
SC-PS152 AfRQ/AfRQ ≈7–14 300–600 16 (2/12) 162 ± 13 100 (9/9) 172 ± 16 100% (7/7)
SC-UCD/99 adapted to BoPrP-Tg110 –- >650 0 (0/13) >650 0 (0/10) ND ND
Healthy sheep brain ARQ/ARQ >650 0 (0/14) >650 0 (0/9) ND ND

*BSE, bovine spongiform encephalopathy; TSE, transmissible spongiform encephalopathy; PrPres, atypical proteinase K–resistant prion protein; dpi, days postinoculation; ND, not determined.
†No. animals positive for the aberrant form of PrP associated with disease / no. inoculated animals.

Main Article

1These authors contributed equally to this article.
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Page updated: October 04, 2010
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