Volume 15, Number 8—August 2009
Research
Transgenic Mice Expressing Porcine Prion Protein Resistant to Classical Scrapie but Susceptible to Sheep Bovine Spongiform Encephalopathy and Atypical Scrapie
Table
Transmission of cattle BSE, sheep BSE, and sheep scrapie isolates in transgenic mice expressing porcine prion protein*
TSE isolate | Genotype | PrPres, kDa | First passage |
Second passage |
Third passage |
|||||
---|---|---|---|---|---|---|---|---|---|---|
Survival time, dpi | % (Attack rate) | Survival time, dpi | % (Attack rate) | Survival time, dpi | % (Attack rate) | |||||
Cattle-BSE1 | – | 19 | >650 | 0 (0/12)† | 269 ± 3 | 100 (10/10) | 204 ± 12 | 100 (9/9) | ||
Cattle-BSE2 | – | 19 | 498 ± 9 | 17 (2/12)† | 198 ± 6 | 100 (15/15) | 193 ± 17 | 100 (6/6) | ||
Cattle-BSE0 | – | 19 | >650 | 19 (3/16)† | 197 ± 4 | 100 (12/12) | 190 ± 10 | 100 (7/7) | ||
Sheep-BSE0 | ARQ/ARQ | 19 | 458 ± 11 | 100 (15/15) | 162 ± 4 | 100 (13/13) | 166 ± 7 | 100 (7/7) | ||
SC-662/97 | ARQ/ARQ | 21 | >650 | 0 (0/10) | >650 | 0 (0/12) | ND | ND | ||
SC-UCD/99 | ARQ/ARQ | 21 | >650 | 0 (0/12) | >650 | 0 (0/9) | ND | ND | ||
SC-Langlade | ARQ/ARQ | 21 | >650 | 0 (0/12) | >650 | 0 (0/12) | ND | ND | ||
SC-PS13 | ARQ/ARQ | 20 | >650 | 0 (0/12) | >650 | 0 (0/12) | ND | ND | ||
SC-JR01 | VRQ/VRQ | 21 | >650 | 0 (0/12) | >650 | 0 (0/12) | ND | ND | ||
SC-PS83 | ARR/ARR | 21 | >650 | 0 (0/12) | >650 | 0 (0/12) | ND | ND | ||
SC-PS48 | VRQ/VRQ | 19 | >650 | 0 (0/9) | >650 | 0 (0/10) | ND | ND | ||
SC-PS152 | AfRQ/AfRQ | ≈7–14 | 300–600 | 16 (2/12) | 162 ± 13 | 100 (9/9) | 172 ± 16 | 100% (7/7) | ||
SC-UCD/99 adapted to BoPrP-Tg110 | –- | >650 | 0 (0/13) | >650 | 0 (0/10) | ND | ND | |||
Healthy sheep brain | ARQ/ARQ | >650 | 0 (0/14) | >650 | 0 (0/9) | ND | ND |
*BSE, bovine spongiform encephalopathy; TSE, transmissible spongiform encephalopathy; PrPres, atypical proteinase K–resistant prion protein; dpi, days postinoculation; ND, not determined.
†No. animals positive for the aberrant form of PrP associated with disease / no. inoculated animals.