Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 15, Number 8—August 2009

Legionella pneumophila in Rainwater on Roads

Ryota Sakamoto, Akira OhnoComments to Author , Toshitaka Nakahara, Kazunari Satomura, Suketaka Iwanaga, Yuuichiro Kouyama, Fumiaki Kura, Naoyuki Kato, Kozo Matsubayashi, Kiyohito Okumiya, and Keizo Yamaguchi
Author affiliations: Kyoto University, Kyoto, Japan (R. Sakamoto, T. Nakahara, K. Satomura, S. Iwanaga, K. Matsubayashi); Toho University School of Medicine, Tokyo, Japan (A. Ohno, Y. Kouyama, N. Kato, K. Yamaguchi); National Institute of Infectious Diseases, Tokyo (F. Kura); Research Institute for Humanity and Nature, Kyoto (K. Okumiya)

Main Article


Legionella spp. and Acanthamoeba spp. isolated from asphalt road sources, Tokyo, Japan, July–October 2007

SourcePositive samples, no. (%)
Primary survey
Puddles on roads (n = 18)
L. pneumophila*7 (38.9)
Acanthamoeba spp.†4 (22.2)
Rainwater (n = 10)
L. pneumophila*0
Legionella spp.‡1 (10.0)
Acanthamoeba spp.†3 (30.0)
Swabs from roads on sunny days (n = 12)
L. pneumophila*0
Legionella spp.‡2 (16.7)
Acanthamoeba spp.†
2 (16.7)
Fixed-point observation of puddles on roads
L. pneumophila concentration (n = 45)
<20 CFU/100 mL29 (64.4)
20–99 CFU/100 mL7 (15.6)
100–999 CFU/100 mL7 (15.6)
>1,000 CFU/100 mL2 ( 4.4)
L. pneumophila serogroups (n = 150 strains isolated)
156 (37.3)
350 (33.3)
215 (10.0)
612 ( 8.0)
511 ( 7.3)
Others6 ( 4.0)

*Tested by using culture method.
†Tested by using PCR method.
‡Tested by using loop-mediated isothermal amplification method.

Main Article

Page created: November 01, 2010
Page updated: November 01, 2010
Page reviewed: November 01, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.