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Volume 15, Number 9—September 2009


Monitoring of Putative Vectors of Bluetongue Virus Serotype 8, Germany

Bernd Hoffmann, Burkhard Bauer, Christian Bauer, Hans-Joachim Bätza, Martin Beer, Peter-Henning Clausen, Martin Geier, Jörn M. Gethmann, Ellen Kiel, Gabriele Liebisch, Arndt Liebisch, Heinz Mehlhorn, Günter A. Schaub, Doreen Werner, and Franz J. ConrathsComments to Author 
Author affiliations: Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany (B. Hoffmann, M. Beer); Free University of Berlin, Berlin, Germany (B. Bauer, P.-H. Clausen); Justus Liebig University Giessen, Giessen, Germany (C. Bauer); Federal Ministry for Food, Agriculture and Consumer Protection, Bonn, Germany (H.-J. Bätza); University of Regensburg, Regensburg, Germany (M. Geier); Friedrich-Loeffler-Institut, Wusterhausen, Germany (J.M. Gethmann, F.J. Conraths); Carl von Ossietzky University Oldenburg, Oldenburg, Germany (E. Kiel); Zecklab, Burgwedel, Germany (G. Liebisch, A. Liebisch); Heinrich-Heine-University of Düsseldorf, Düsseldorf, Germany (H. Mehlhorn); Ruhr University, Bochum, Germany (G.A. Schaub); Leibniz-Center for Agricultural Landscape Research e. V, Müncheberg, Germany (D. Werner)

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Table 2

Genetic characterization of batches of midges of the Culicoides pulicaris group, Germany, April 2007–May 2008*

Batch no. rRT-PCR, Ct C. pulicaris s.s. C. punctatus C. impunctatus C. grisescens C. newsteadi
263/49 A 28.57 ++ + +
263/49 B 29.46 +++ +++ ++ + +
276/46B 31.37 +++ + +++
276/62A 34.43 +++ +++
292/4A 33.97 +++ +++
304/63A 32.90 +++ +++ + ++

*rRT-PCR, real-time reverse transcription–PCR; Ct, cycle threshold; –, no visible band; +, faint; ++, distinct; +++, strong band after agarose gel electrophoresis of PCR products and staining with ethidium bromide.

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