Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 16, Number 1—January 2010

Worldwide Dissemination of the blaOXA-23 Carbapenemase Gene of Acinetobacter baumannii1

Pauline D. Mugnier, Laurent Poirel, Thierry Naas, and Patrice NordmannComments to Author 
Author affiliations: Institut National de la Santé et de la Recherche Médicale Unité 914, Paris, France; Université Paris Sud, Bicêtre, France; 1This work was presented in part at the 19th European Congress of Clinical Microbiology and Infectious Diseases, Helsinki, Finland, May 16–19, 2009.

Main Article

Figure 2

Pulsed-field electrophoresis (PFGE) profiles of ApaI-digested genomic DNA from strains of Acinetobacter baumannii. PFGE types, European clone types, and multilocus sequence typing (MLST) results are shown. *ST, sequence type determined by Bartual et al. (19) compared with ST determined by Nemec et al. (20). Lane M, molecular size markers (48.5 kb).

Figure 2. Pulsed-field electrophoresis (PFGE) profiles of ApaI-digested genomic DNA from strains of Acinetobacter baumannii. PFGE types, European clone types, and multilocus sequence typing (MLST) results are shown. *ST, sequence type determined by Bartual et al. (19) compared with ST determined by Nemec et al. (20). Lane M, molecular size markers (48.5 kb).

Main Article

  1. Perez  F, Hujer  AM, Hujer  KM, Decker  BK, Rather  PN, Bonomo  RA. Global challenge of multidrug-resistant Acinetobacter baumannii. Antimicrob Agents Chemother. 2007;51:347184. DOIPubMedGoogle Scholar
  2. Peleg  AY, Seifert  H, Paterson  DL. Acinetobacter baumannii: emergence of a successful pathogen. Clin Microbiol Rev. 2008;21:53882. DOIPubMedGoogle Scholar
  3. Poirel  L, Nordmann  P. Carbapenem resistance in Acinetobacter baumannii: mechanism and epidemiology. Clin Microbiol Infect. 2006;12:82636. DOIPubMedGoogle Scholar
  4. Scaife  W, Young  HK, Paton  RH, Amyes  GB. Transferable imipenem-resistance in Acinetobacter species from a clinical source. J Antimicrob Chemother. 1995;36:5857. DOIPubMedGoogle Scholar
  5. Poirel  L, Figueiredo  S, Cattoir  V, Carattoli  A, Nordmann  P. Acinetobacter radioresistens as a silent source of carbapenem resistance for Acinetobacter spp. Antimicrob Agents Chemother. 2008;52:12526. DOIPubMedGoogle Scholar
  6. Stoeva  T, Higgins  PG, Bojkova  K, Seifert  H. Clonal spread of carbapenem-resistant OXA-23 positive Acinetobacter baumannii in a Bulgarian university hospital. Clin Microbiol Infect. 2008;14:7237. DOIPubMedGoogle Scholar
  7. Zhou  H, Pi  BR, Yang  Q, Yu  YS, Chen  YG, Li  LJ, Dissemination of imipenem-resistant Acinetobacter baumannii strains carrying the ISAba1 blaOXA-23 gene in a Chinese hospital. J Med Microbiol. 2007;56:107680. DOIPubMedGoogle Scholar
  8. Carvalho  KR, Carvalho-Assef  AP, Peirano  G, Santos  LC, Pereira  MJ, Asensi  MD. Dissemination of multidrug-resistant Acinetobacter baumannii genotypes carrying blaOXA-23 collected from hospitals in Rio de Janeiro, Brazil. Int J Antimicrob Agents. 2009;34:258. DOIPubMedGoogle Scholar
  9. Calhoun  JH, Murray  CK, Manring  MM. Multidrug-resistant organisms in military wounds from Iraq an Afghanistan. Clin Orthop Relat Res. 2008;466:135662. DOIPubMedGoogle Scholar
  10. Naas  T, Levy  M, Hirschauer  C, Marchandin  H, Nordmann  P. Outbreak of carbapenem OXA-23 in a tertiary care hospital of Papeete, French Polynesia. J Clin Microbiol. 2005;43:48269. DOIPubMedGoogle Scholar
  11. Corvec  S, Poirel  L, Naas  T, Drugeon  H, Nordmann  P. Genetics and expression of the carbapenems-hydrolysing oxacillinase gene blaOXA-23 in Acinetobacter baumannii. Antimicrob Agents Chemother. 2007;51:15303. DOIPubMedGoogle Scholar
  12. Adams-Haduch  JM, Paterson  DL, Sidjabat  HE, Pasculle  AW, Potoski  BA, Muto  CA, Genetic basis of multidrug resistance in Acinetobacter baumannii clinical isolates at a tertiary medical center in Pennsylvania. Antimicrob Agents Chemother. 2008;52:383743. DOIPubMedGoogle Scholar
  13. Mugnier  PD, Poirel  L, Nordmann  P. Functional analysis of insertion sequence ISAba1, responsible for genomic plasticity of Acinetobacter baumannii. J Bacteriol. 2009;191:24148. DOIPubMedGoogle Scholar
  14. Mugnier  P, Poirel  L, Pitout  M, Nordmann  P. Carbapenem-resistant and OXA-23 producing Acinetobacter baumannii isolates in the United Arab Emirates. Clin Microbiol Infect. 2008;14:87982. DOIPubMedGoogle Scholar
  15. Mugnier  PD, Bindayna  KM, Poirel  L, Nordmann  P. Diversity of plasmid-mediated carbapenem-hydrolysing oxacillinases among carbapenem-resistant Acinetobacter baumannii isolates from Kingdom of Bahrain. J Antimicrob Chemother. 2009;63:10713. DOIPubMedGoogle Scholar
  16. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; 18th informational supplement. M100–S18. Wayne (PA): The Institute; 2008.
  17. Dortet  L, Legrand  P, Soussy  CJ, Cattoir  V. Bacterial identification, clinical significance, and antimicrobial susceptibilities of Acinetobacter ursingii and Acinetobacter schindleri, two frequently misidentified opportunistic pathogens. J Clin Microbiol. 2006;44:44718. DOIPubMedGoogle Scholar
  18. Turton  JF, Gabriel  SN, Valderrey  C, Kaufmann  ME, Pitt  TL. Use of sequence-based typing and multiplex PCR to identify clonal lineages of outbreak strains of Acinetobacter baumannii. Clin Microbiol Infect. 2007;13:80725. DOIPubMedGoogle Scholar
  19. Bartual  SG, Seifert  H, Hippler  C, Luzon  MA, Wisplinghoff  H, Rodriguez-Valera  F. Development of a multilocus sequence typing scheme for characterization of clinical isolates of Acinetobacter baumannii. J Clin Microbiol. 2005;43:438290. DOIPubMedGoogle Scholar
  20. Nemec  A, Krizova  L, Maixnerova  M, Diancourt  L, Van der Reijden  TJK, Brisse  S, Emergence of carbapenem resistance in Acinetobacter baumannii in the Czech Republic is associated with the spread of multidrug resistant strains of European clone II. J Antimicrob Chemother. 2008;62:4849. DOIPubMedGoogle Scholar
  21. Liu  SL, Hessel  A, Sanderson  KE. Genomic mapping with I-CeuI, an intron-encoded endonuclease specific for genes for ribosomal RNA, in Salmonella spp., Escherichia coli, and other bacteria. Proc Natl Acad Sci U S A. 1993;90:68748. DOIPubMedGoogle Scholar
  22. Poirel  L, Guibert  M, Bellais  S, Naas  T, Nordmann  P. Integron- and carbenicillinase-mediated reduced susceptibility to amoxicillin–clavulanic acid in isolates of multidrug-resistant Salmonella enterica serotype typhimurium DT104 from French patients. Antimicrob Agents Chemother. 1999;43:1098104.PubMedGoogle Scholar
  23. Tenover  FC, Arbeit  RD, Goering  RV, Mickelsen  PA, Murray  BE, Persing  DH, Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol. 1995;33:22339.PubMedGoogle Scholar
  24. Bogaerts  P, Cuzon  G, Naas  T, Bauraing  C, Deplano  A, Lissoir  B, Carbapenem-resistant Acinetobacter baumannii isolates expressing the blaOXA-23 gene associated with ISAba4 in Belgium. Antimicrob Agents Chemother. 2008;52:42056. DOIPubMedGoogle Scholar
  25. Park  YK, Choi  JY, Jung  SI, Park  KH, Lee  H, Jung  DS, Two distinct clones of carbapenem-resistant Acinetobacter baumannii isolates from Korean hospitals. Diagn Microbiol Infect Dis. 2009;64:38995. DOIPubMedGoogle Scholar

Main Article

Page created: March 31, 2011
Page updated: March 31, 2011
Page reviewed: March 31, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.