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Volume 16, Number 1—January 2010
Dispatch

Enterotoxigenic Escherichia coli Multilocus Sequence Types in Guatemala and Mexico

Matilda NicklassonComments to Author , John D. Klena, Claudia Rodas, August Louis Bourgeois, Olga Torres, Ann-Mari Svennerholm, and Åsa Sjöling
Author affiliations: University of Gothenburg, Gothenburg, Sweden (M. Nicklasson, C. Rodas, A.-M. Svennerholm, Å. Sjöling); United States Naval Medical Research Unit 3, Cairo, Egypt (J. Klena); Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA (A.L. Bourgeois); Instituto de Nutrición de Centro América y Panamá, Guatemala City, Guatemala (O. Torres)

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Figure

Dendrogram of the 24 enterotoxigenic Escherichia coli strains from Guatemala and Mexico included in the study, showing multilocus sequence type. Sequences were assembled with BioEdit and aligned using ClustalX within BioEdit (12). The dendogram represents the relationship of a concatenation of the sequences from each strain and was constructed by using MEGA 3.1 (13). Phylogenetic reconstructions were created by using the neighbor-joining method with the Kimura 2-parameter substitution model, usi

Figure. Dendrogram of the 24 enterotoxigenic Escherichia coli strains from Guatemala and Mexico included in the study, showing multilocus sequence type. Sequences were assembled with BioEdit and aligned using ClustalX within BioEdit (12). The dendogram represents the relationship of a concatenation of the sequences from each strain and was constructed by using MEGA 3.1 (13). Phylogenetic reconstructions were created by using the neighbor-joining method with the Kimura 2-parameter substitution model, using 1,000 bootstrap replicates. A similar arrangement of the strains was indicated by eBURST version 2 analysis (http://eburst.mlst.net). Scale bar indicates dissimilarity, where 0 is completely identical and 1 is completely dissimilar.

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References
  1. Qadri  F, Svennerholm  A-M, Faruque  AS, Sack  RB. Enterotoxigenic Escherichia coli in developing countries: epidemiology, microbiology, clinical features, treatment, and prevention. Clin Microbiol Rev. 2005;18:465–83. DOIPubMedGoogle Scholar
  2. Wolf  MK. Occurence, distribution, and associations of O and H serogroups, colonization factor antigens, and toxins of enterotoxigenic Escherichia coli. Clin Microbiol Rev. 1997;10:569–84.PubMedGoogle Scholar
  3. Bölin  I, Wiklund  G, Qadri  F, Torres  O, Bourgeois  AL, Savarino  S, Enterotoxigenic Escherichia coli with STh and STp genotypes is associated with diarrhea both in children in areas of endemicity and in travelers. J Clin Microbiol. 2006;44:3872–7. DOIPubMedGoogle Scholar
  4. Gaastra  W, Svennerholm  A-M. Colonization factors of human enterotoxigenic Escherichia coli (ETEC). Trends Microbiol. 1996;4:444–52. DOIPubMedGoogle Scholar
  5. Sack  DA, Shimko  J, Torres  O, Bourgeois  AL, Francia  DS, Gustafsson  B, Randomised, double-blind, safety and efficacy of a killed oral vaccine for enterotoxigenic E. coli diarrhoea of travellers to Guatemala and Mexico. Vaccine. 2007;25:4392–400. DOIPubMedGoogle Scholar
  6. Shaheen  HI, Abdel Messih  IA, Klena  JD, Mansour  A, El-Wakkeel  Z, Wierzba  TF, Phenotypic and genotypic analysis of enterotoxigenic Escherichia coli in samples obtained from Egyptian children presenting to referral hospitals. J Clin Microbiol. 2009;47:189–97. DOIPubMedGoogle Scholar
  7. Qadri  F, Saha  A, Ahmed  T, Al Tarique  A, Ara Begum  Y, Svennerholm  A-M. Disease burden due to enterotoxigenic Escherichia coli in the first 2 years of life in an urban community in Bangladesh. Infect Immun. 2007;75:3961–8. DOIPubMedGoogle Scholar
  8. Bourgeois  AL, Halpern  J, Gustafsson  B, Svennerholm  A-M, Torres  O, Belkind-Gerson  J, Protective efficacy of an oral killed vaccine (OKV) for enterotoxigenic E. coli (ETEC) in adult travelers to Guatemala (GU) and Mexico (MX). 45th Annual Meeting of the Interscience Conference on Antimicrobial Agents and Chemotherapy. 2005.
  9. Taylor  DN, Bourgeois  AL, Ericsson  CD, Steffen  R, Jiang  ZD, Halpern  J, A randomized, double-blind, multicenter study of rifaximin compared with placebo and with ciprofloxacin in the treatment of travelers’ diarrhea. Am J Trop Med Hyg. 2006;74:1060–6.PubMedGoogle Scholar
  10. Sjöling  Å, Wiklund  G, Savarino  SJ, Cohen  DI, Svennerholm  A-M. Comparative analyses of phenotypic and genotypic methods for detection of enterotoxigenic Escherichia coli (ETEC) toxins and colonisation factors. J Clin Microbiol. 2007;45:3295–301. DOIPubMedGoogle Scholar
  11. Wirth  T, Falush  D, Lan  R, Colles  F, Mensa  P, Wieler  LH, Sex and virulence in Escherichia coli: an evolutionary perspective. Mol Microbiol. 2006;60:1136–51. DOIPubMedGoogle Scholar
  12. Hall  TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser. 1999;41:95–8.
  13. Kumar  S, Tamura  K, Nei  M. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform. 2004;5:150–63. DOIPubMedGoogle Scholar
  14. Nicklasson  M, Sjöling  Å, Lebens  M, Tobias  J, Janzon  A, Brive  L, Mutations in the periplasmic chaperone leading to loss of surface expression of the colonization factor CS6 in enterotoxigenic Escherichia coli (ETEC) clinical isolates. Microb Pathog. 2008;44:246–54. DOIPubMedGoogle Scholar
  15. Wolf  MK, de Haan  LA, Cassels  FJ, Willshaw  GA, Warren  R, Boedeker  EC, The CS6 colonization factor of human enterotoxigenic Escherichia coli contains two heterologous major subunits. FEMS Microbiol Lett. 1997;148:35–42. DOIPubMedGoogle Scholar

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