Volume 16, Number 12—December 2010
Research
Eastern Equine Encephalitis Virus in Mosquitoes and Their Role as Bridge Vectors
Philip M. Armstrong
and Theodore G. Andreadis
and Theodore G. Andreadis
Table
Eastern equine encephalitis virus isolated and virus titers from mosquitoes obtained in Connecticut, USA, 2009*
| Mosquito species | No. mosquitoes collected | No. virus isolates | Infection rate/1,000 mosquitoes, MLE (95% CI) | Mean Ct by qRT-PCR | Mean titer log10 PFU/ mosquito pool | % Mosquito pools >3.0 log10 PFU/ mosquito pool |
|---|---|---|---|---|---|---|
| Aedes cinereus | 15,294 | 6 | 0.4 (0.2–0.8) | 34.0 | 2.92 | 16.7 |
| Ae. vexans | 26,462 | 2 | 0.1 (0–0.2) | 33.0 | 1.15 | 0 |
| Anopheles punctipennis | 5,573 | 4 | 0.7 (0.2–1.7) | 34.6 | 1.69 | 0 |
| An. quadrimaculatus | 607 | 2 | 3.3 (0.6–10.6) | 35.9 | 1.67 | 0 |
| An. walkeri | 2,381 | 2 | 0.8 (0.2–2.7) | 35.1 | 1.43 | 0 |
| Culex restuans | 14,609 | 1 | 0.1 (0–0.3) | 35.2 | 0.85 | 0 |
| Cx. salinarius | 12,605 | 3 | 0.2 (0.1–0.6) | 32.1 | 1.31 | 0 |
| Culiseta melanura | 25,595 | 83 | 3.4 (2.8–4.2) | 22.3 | 6.55 | 88.0 |
| Ochlerotatus canadensis | 40,543 | 10 | 0.2 (0.1–0.4) | 32.9 | 2.82 | 10.0 |
| Oc. cantator | 4,457 | 1 | 0.2 (0–1.1) | 31.8 | 1.54 | 0 |
| Oc. triseriatus | 3,000 | 1 | 0.3 (0–1.6) | >50 | 1.60 | 0 |
| Oc. trivittatus | 23,340 | 2 | 0.1 (0–0.3) | 38.8 | <0.8 | 0 |
| Psorophora ferox | 13,677 | 1 | 0.1 (0–0.35) | 34.0 | <0.8 | 0 |
| Uranotaenia sapphirina | 2,954 | 4 | 1.4 (0.4–3.2) | 36.5 | 1.00 | 0 |
| Remaining species† | 100,544 | 0 | – | – | – | – |
*MLE, maximum-likelihood estimation; CI, confidence interval; Ct, cycle threshold; qRT-PCR, quantitative reverse transcription–PCR; –, not applicable. Mean Ct and PFU values were calculated for mosquito pools positive by qRT-PCR and plaque titration.
†n = 21.
Page created: August 29, 2011
Page updated: August 29, 2011
Page reviewed: August 29, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.