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Volume 16, Number 12—December 2010
Dispatch

Mycobacterium tuberculosis Infection of Domesticated Asian Elephants, Thailand

Taweepoke Angkawanish, Worawidh WajjwalkuComments to Author , Anucha Sirimalaisuwan, Thattawan Kaewsakhorn, Kittikorn Boonsri, Victor P.M.G. Rutten, and SittidetMahasawangkul
Author affiliations: Author affiliations: National Elephant Institute, Forest Industry Organization, Lampang, Thailand (T. Angkawanish, S. Mahasawangkul); Kasetsart University, Nakhonpathom, Thailand (W. Wajjwalku); Chiang Mai University, Chiang Mai, Thailand (A. Sirimalaisuwan, T. Kaewsakhorn, K. Boonsri); Utrecht University, Utrecht, the Netherlands (T. Angkawanish, V.P.M.G. Rutten); University of Pretoria, Onderstepoort, South Africa (V.P.M.G. Rutten)

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Table 1

Primers used to identify bacteria cultured from trunk wash and tissue samples from domesticated Asian elephants, Thailand, 2003–2008*

Primer Forward Reverse
16s rRNA 5′-AgA gTT TgA TCC Tgg CTC Ag-3′ 5′-ACg gCT ACC TTg TTA CgA CTT-3′
ITS 5′-TTg TAC ACA CCg CCg gTC a-3′ 5′-TCT CgA TgC CAA ggC ATC CAC C-3′
gyrB 5′-TCG GAC GCG TAT GCG ATA TC-3′ 5′-ACA TAC AGT TCG GAC TTG CG-3′

*ITS modified from (7,8); gyrB modified from (9). ITS, internal transcribed spacer; gyrB, gyrase B.

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