Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 16, Number 2—February 2010
Letter

Mycobacterium tuberculosis Beijing Strain, Bamako, Mali

Bassirou Diarra1, Sophia Siddiqui1Comments to Author , Dramane Sogoba, Brehima Traore, Mamoudou Maiga, Janice Washington, Anatole Tounkara, and Michael A. Polis
Author affiliations: Project SEREFO-NIAID/University of Bamako Research Collaboration on HIV/TB, Bamako, Mali (B. Diarra, D. Sogoba, B. Traore, M. Maiga, A. Tounkara); National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA (S. Siddiqui, M.A. Polis); US Food and Drug Administration, Silver Spring, Maryland, USA (J. Washington); 1These authors contributed equally to this article.

Main Article

Appendix Figure

A) Identification of isolates of the Mycobacterium tuberculosis Beijing clade (6) by using spoligotyping. The spoligotype pattern of the M. tuberculosis Beijing clade is characterized by the absence of hybridization of spacers 1–34 as shown, in combination with hybridization of spacers 35–43. Negative control (Neg.) shows absence of all spacers. For comparison, H37Rv (a laboratory strain) and M. bovis BCG show different patterns of spacers. Also shown are other clades not identified. B) Restric

Appendix Figure. A) Identification of isolates of the Mycobacterium tuberculosis Beijing clade (6) by using spoligotyping. The spoligotype pattern of the M. tuberculosis Beijing clade is characterized by the absence of hybridization of spacers 1–34 as shown, in combination with hybridization of spacers 35–43. Negative control (Neg.) shows absence of all spacers. For comparison, H37Rv (a laboratory strain) and M. bovis BCG show different patterns of spacers. Also shown are other clades not identified. B) Restriction fragment length polymorphism (7) patterns of strains from the 2 patients. Patterns confirm that both strains belong to the Beijing clade. However, patterns indicate different strains and confirm lack of direct transmission between the patients. The 2 additional clades shown (not from these patients) illustrate differences between the Beijing and other clades.

Main Article

References
  1. Filliol  I, Driscoll  JR, van Soolingen  D, Kreiswirth  BN, Kremer  K, Valétudie  G, Global distribution of Mycobacterium tuberculosis spoligotypes. Emerg Infect Dis. 2002;8:13479.PubMed
  2. European Concerted Action on New Generation Genetic Markers and Techniques for the Epidemiology and Control of Tuberculosis. Beijing/W genotype Mycobacterium tuberculosis and drug resistance. Emerg Infect Dis. 2006;12:73643.PubMed
  3. van Soolingen  D, Qian  L, de Haas  PE, Douglas  JT, Traore  H, Portaels  F, Predominance of a single genotype of Mycobacterium tuberculosis in countries of east Asia. J Clin Microbiol. 1995;33:32348.PubMed
  4. Glynn  JR, Whiteley  J, Bifani  PJ, Kremer  K, van Soolingen  D. Worldwide occurrence of Beijing/W strains of Mycobacterium tuberculosis: a systematic review. Emerg Infect Dis. 2002;8:8439.PubMed
  5. Bifani  PJ, Mathema  B, Kurepina  NE, Kreiswirth  BN. Global dissemination of the Mycobacterium tuberculosis W-Beijing family strains [review]. Trends Microbiol. 2002;10:4552. DOIPubMed
  6. Kremer  K, Glynn  JR, Lillebaek  T, Niemann  S, Kurepina  NE, Kreiswirth  BN, Definition of the Beijing/W lineage of Mycobacterium tuberculosis on the basis of genetic markers. J Clin Microbiol. 2004;42:40409. DOIPubMed
  7. Van Embden  JD, Cave  MD, Crawford  JT, Dale  JW, Eisenach  KD, Gicquel  B, Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol. 1993;31:4069.PubMed
  8. van der Spuy  GD, Kremer  K, Ndabambi  SL, Beyers  N, Dunbar  R, Marais  BJ, Changing Mycobacterium tuberculosis population highlights clade-specific pathogenic characteristics. Tuberculosis (Edinb). 2009;89:1205. Epub 2008 Dec 2. DOIPubMed
  9. Werngren  J, Hoffner  SE. Drug-susceptible Mycobacterium tuberculosis Beijing genotype does not develop mutation-conferred resistance to rifampin at an elevated rate. J Clin Microbiol. 2003;41:15204. DOIPubMed
  10. Cowley  D, Govender  D, February  B, Wolfe  M, Steyn  L, Evans  J, Recent and rapid emergence of W-Beijing strains of Mycobacterium tuberculosis in Cape Town, South Africa. Clin Infect Dis. 2008;47:12529. DOIPubMed

Main Article

Page created: December 10, 2010
Page updated: December 10, 2010
Page reviewed: December 10, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external