Experimental Infection of Squirrel Monkeys with Nipah Virus

Philippe Marianneau¹, Vanessa Guillaume¹, K. Thong Wong, Munisamy Badmanathan, Ren Yih Looi, Séverine Murri, Philippe Loth, Noël Tordo, T. Fabian Wild, Branka Horvat

, and Hugues Contamin

Author affiliations: Institut Pasteur, Lyon, France (P. Marianneau, S. Murri, P. Loth, N. Tordo, H. Contamin); Institut national de santé et de la recherché médicale, Lyon (V. Guillaume, T.F. Wild, B. Horvat); University of Malaya, Kuala Lumpur, Malaysia (K.T. Wong, M. Badmanathan, R.Y. Looi)

Main Article

Table 2

Detection of anti–Nipah virus antibodies in 12 squirrel monkeys by ELISA and seroneutralization assay*

Monkey	Mode of infection†	Serology‡	Day postinfection
Monkey	Mode of infection†	Serology‡	2	3 or 4	8 or 9	12	17	30	37	52 or 56
A§	IV 10³	IgM	–	Neg
	IV 10³	IgG	–	Neg
	IV 10³	Neutralization	–	Neg
B¶	IV 10³	IgM	Neg	–	Neg	0.396
	IV 10³	IgG	Neg	–	Neg	Neg
	IV 10³	Neutralization	Neg	–	Neg	Neg
C¶	IV 10³	IgM	Neg	–	0.664	–	–
	IV 10³	IgG	Neg	–	Neg	–	–
	IV 10³	Neutralization	Neg	–	Neg	–	–
D§	IV 10⁷	IgM	–	Neg
	IV 10⁷	IgG	–	Neg
	IV 10⁷	Neutralization	–	Neg
E¶	IV 10⁷	IgM	Neg	–	1.343
	IV 10⁷	IgG	Neg	–	0.562
	IV 10⁷	Neutralization	Neg	–	40
F	IV 10⁷	IgM	Neg	–	1.550	–	–	0.374	–	0.175
	IV 10⁷	IgG	Neg	–	0.369	–	–	2.867	–	3.023
	IV 10⁷	Neutralization	Neg	–	80	–	–	>1,280	–	>1,280
G§	IN 10³	IgM	Neg	Neg
	IN 10³	IgG	Neg	Neg
	IN 10³	Neutralization	Neg	Neg
H	IN 10³	IgM	–	–	Neg	–	–	Neg	–	Neg
	IN 10³	IgG	–	–	Neg	–	–	Neg	–	Neg
	IN 10³	Neutralization	–	–	Neg	–	–	Neg	–	Neg
I	IN 10³	IgM	–	–	Neg	–	–	Neg	Neg	Neg
	IN 10³	IgG	–	–	Neg	–	–	Neg	Neg	Neg
	IN 10³	Neutralization	–	–	Neg	–	–	Neg	Neg	Neg
J§	IN 10⁷	IgM	–	Neg
	IN 10⁷	IgG	–	Neg
	IN 10⁷	Neutralization	–	Neg
K	IN 10⁷	IgM	Neg	–	–	–	0.286
	IN 10⁷	IgG	Neg	–	–	–	1.757
	IN 10⁷	Neutralization	Neg	–	–	–	80
L	IN 10⁷	IgM	–	–	0.375			0.248		Neg
	IN 10⁷	IgG	–	–	Neg			2.078		2.308
	IN 10⁷	Neutralization	–	–	Neg			320		320

*IV, intravenous; Ig, immunoglobulin; IN, intranasal; dpi, days postinfection.
†Mode of infection and delivered viral dose (in PFU).
‡IgG and IgM antibodies were determined by ELISA and results are presented as absorbance readings. Neutralization titers are expressed as reciprocal values of 2-fold serum dilutions required to completely inhibit cytopathic effect of 25 PFU of Nipah virus on Vero cells.
§Early systemic euthanasia: 3 dpi (A and D) or 4 dpi (G and J).
¶Death caused by Nipah virus infection, B: 12 dpi, C: 21 dpi, and E: 8 dpi.

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¹These authors contributed equally to this article.

The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.

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Page created: December 14, 2010
Page last updated: December 14, 2010
Page last reviewed: December 14, 2010
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