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Volume 16, Number 3—March 2010

Dispatch

Experimental Infection of Squirrel Monkeys with Nipah Virus

Philippe Marianneau1, Vanessa Guillaume1, K. Thong Wong, Munisamy Badmanathan, Ren Yih Looi, Séverine Murri, Philippe Loth, Noël Tordo, T. Fabian Wild, Branka HorvatComments to Author , and Hugues Contamin
Author affiliations: Institut Pasteur, Lyon, France (P. Marianneau, S. Murri, P. Loth, N. Tordo, H. Contamin); Institut national de santé et de la recherché médicale, Lyon (V. Guillaume, T.F. Wild, B. Horvat); University of Malaya, Kuala Lumpur, Malaysia (K.T. Wong, M. Badmanathan, R.Y. Looi)

Main Article

Table 2

Detection of anti–Nipah virus antibodies in 12 squirrel monkeys by ELISA and seroneutralization assay*

Monkey Mode of infection† Serology‡ Day postinfection
2 3 or 4 8 or 9 12 17 30 37 52 or 56
A§ IV 103 IgM – Neg
IV 103 IgG – Neg
IV 103
 Neutralization
 –
 Neg
B¶ IV 103 IgM Neg – Neg 0.396
IV 103 IgG Neg – Neg Neg
IV 103
 Neutralization
 Neg
 –
 Neg
 Neg
C¶ IV 103 IgM Neg – 0.664 – –
IV 103 IgG Neg – Neg – –
IV 103
 Neutralization
 Neg
 –
 Neg
 –
 –
D§ IV 107 IgM – Neg
IV 107 IgG – Neg
IV 107
 Neutralization
 –
 Neg
E¶ IV 107 IgM Neg – 1.343
IV 107 IgG Neg – 0.562
IV 107
 Neutralization
 Neg
 –
 40
F IV 107 IgM Neg – 1.550 – – 0.374 – 0.175
IV 107 IgG Neg – 0.369 – – 2.867 – 3.023
IV 107
 Neutralization
 Neg
 –
 80
 –
 –
 >1,280
 –
 >1,280
G§ IN 103 IgM Neg Neg
IN 103 IgG Neg Neg
IN 103
 Neutralization
 Neg
 Neg
H IN 103 IgM – – Neg – – Neg – Neg
IN 103 IgG – – Neg – – Neg – Neg
IN 103
 Neutralization
 –
 –
 Neg
 –
 –
 Neg
 –
 Neg
I IN 103 IgM – – Neg – – Neg Neg Neg
IN 103 IgG – – Neg – – Neg Neg Neg
IN 103
 Neutralization
 –
 –
 Neg
 –
 –
 Neg
 Neg
 Neg
J§ IN 107 IgM – Neg
IN 107 IgG – Neg
IN 107
 Neutralization
 –
 Neg
K IN 107 IgM Neg – – – 0.286
IN 107 IgG Neg – – – 1.757
IN 107
 Neutralization
 Neg
 –
 –
 –
 80
L IN 107 IgM – – 0.375 0.248 Neg
IN 107 IgG – – Neg 2.078 2.308
IN 107 Neutralization – – Neg 320 320

*IV, intravenous; Ig, immunoglobulin; IN, intranasal; dpi, days postinfection.
†Mode of infection and delivered viral dose (in PFU).
‡IgG and IgM antibodies were determined by ELISA and results are presented as absorbance readings. Neutralization titers are expressed as reciprocal values of 2-fold serum dilutions required to completely inhibit cytopathic effect of 25 PFU of Nipah virus on Vero cells.
§Early systemic euthanasia: 3 dpi (A and D) or 4 dpi (G and J).
¶Death caused by Nipah virus infection, B: 12 dpi, C: 21 dpi, and E: 8 dpi.

Main Article

1These authors contributed equally to this article.

Page created: December 14, 2010
Page updated: December 14, 2010
Page reviewed: December 14, 2010
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