Volume 16, Number 7—July 2010
Research
Human Infection with Rickettsia felis, Kenya
Allen L. Richards, Ju Jiang, Sylvia Omulo, Ryan Dare, Khalif Abdirahman, Abdile Ali, Shanaaz K. Sharif, Daniel R. Feikin, Robert F. Breiman, and M. Kariuki Njenga
Table 1
Molecular detection results and characteristics of rickettsial DNA preparations in acute-phase serum samples from 6 patients who had fever, Garissa Provincial Hospital, North Eastern Province, Kenya, 2006–2008*
| Patient no. | 17-kDa gene sequence | ompB sequence | RF plasmid |
|---|---|---|---|
| 1 | 100% identification with Rickettsia felis | 100% identification with R. felis | 100% identification with R. felis |
| 2 | 100% identification with R. felis | – | – |
| 3 | – | – | – |
| 4 | – | 100% identification with R. felis | – |
| 5 | – | – | – |
| 6 | 100% identification with R. felis | – | – |
*ompB, outer membrane protein B gene. All samples were positive by Rick17 PCR and Rfelis qPCR and negative by Trick quantitative PCR (qPCR), RF plasmid, and RFδ plasmid. Rick17 qPCR is specific for the 17-kDa antigen gene of Rickettsia species; Trick qPCR is specific for a portion of ompB that is common to tick-borne rickettsiae; Rfelis qPCR is specific for a portion of ompB that is common to R. felis.
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