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Volume 16, Number 8—August 2010

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Not-So-Novel Michigan Rabbit Calicivirus

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EID Abrantes J, Esteves PJ. Not-So-Novel Michigan Rabbit Calicivirus. Emerg Infect Dis. 2010;16(8):1331-1332. https://dx.doi.org/10.3201/eid1608.091803
AMA Abrantes J, Esteves PJ. Not-So-Novel Michigan Rabbit Calicivirus. Emerging Infectious Diseases. 2010;16(8):1331-1332. doi:10.3201/eid1608.091803.
APA Abrantes, J., & Esteves, P. J. (2010). Not-So-Novel Michigan Rabbit Calicivirus. Emerging Infectious Diseases, 16(8), 1331-1332. https://dx.doi.org/10.3201/eid1608.091803.

To the Editor: A disease outbreak in a Michigan rabbitry led Bergin et al. (1) to identify a new rabbit calicivirus distinct from rabbit hemorrhagic disease virus, which they designated as Michigan rabbit calicivirus (MRCV). They found that in domestic rabbits from the United States, 2 different forms of rabbit calicivirus with differences in pathogenicity are circulating. Bergin et al. showed that, phylogenetically, MRCV was more closely related to the nonpathogenic rabbit calicivirus (RCV) than to pathogenic strains and used this observation as an argument for its classification as a novel calicivirus. However, they did not include the publicly available sequences of other nonpathogenic strains, such as Ashington (97% of the capsid viral protein [VP] 60) and the newly identified Lagovirus spp. RCV-A1 (complete genome) (2).

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Thumbnail of Evolutionary relationships of Lagovirus strains. The evolutionary history was inferred by using the neighbor-joining method (3) with the pairwise deletion option. The tree is drawn to scale. There were a total of 563 positions (97% of the capsid viral protein [60 aa sequence]). Phylogenetic analyses were conducted in MEGA 4 (4). Reliability of the tree was assessed by bootstrap with 1,000 replicates and is indicated in the nodes (only relevant values are shown). Several genetic dist

Figure. Evolutionary relationships of Lagovirus strains. The evolutionary history was inferred by using the neighbor-joining method (3) with the pairwise deletion option. The tree is drawn to scale. There were...

Using the same dataset as Bergin et al. and including these sequences, we performed genetic analyses focusing mainly on the capsid VP60. The lack of information for open reading frame 1 for the nonpathogenic strains led to this option. Independently of the sequences’ length, RCV-A1 was more closely related to the Lagovirus spp. European brown hare syndrome virus, here used as an outgroup, and clearly apart from a highly supported primary group that was further subdivided into 2 also highly supported subgroups, 1 composed of pathogenic rabbit hemorrhagic disease virus strains and another encompassing the RCV-like group (RCV, Ashington and Lambay [2], and MRCV). Here, only the phylogenetic tree that corresponds to the more complete VP60 sequences is shown (Figure).

We conclude that MRCV is not a novel calicivirus but a new variant of the nonpathogenic RCV-like group. However, the low pathogenicity presented by MRCV and the presence of viral RNA in the liver rather than in the intestine are clearly new features among the nonpathogenic RCV-like group (5).

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Acknowledgment

This work was supported by Foundation for Science and Technology Portugal grants SFRH/BD/31048/2006 and SFRH/BPD/27021/2006 to J.A. and P.J.E., respectively.

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Joana Abrantes and Pedro J. Esteves

Author affiliations: CIBIO-UP (Centro de Investigação em Biodiversidade e Recursos Genéticos, Vairão–Universidade do Porto, Porto, Portugal (J. Abrantes, P.J. Esteves); Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 892, Université de Nantes, Nantes, France (J. Abrantes); CESPU (Cooperativa de Ensino Superior Politécnico e Universitário), Gandra, Portugal (P.J. Esteves).

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References

  1. Bergin  IL, Wise  AG, Bolin  SR, Mullaney  TP, Kiupel  M, Maes  RK. Novel calicivirus identified in rabbits, Michigan, USA. Emerg Infect Dis. 2009;15:195562. DOIPubMed
  2. Strive  T, Wright  JD, Robinson  AJ. Identification and partial characterisation of a new lagovirus in Australian wild rabbits. Virology. 2009;384:97105. DOIPubMed
  3. Saitou  N, Nei  M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4:40625.PubMed
  4. Tamura  K, Dudley  J, Nei  M, Kumar  S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007;24:15969. DOIPubMed
  5. Capucci  L, Fusi  P, Lavazza  A, Pacciarini  ML, Rossi  C. Detection and preliminary characterization of a new rabbit calicivirus related to rabbit hemorrhagic disease virus but nonpathogenic. J Virol. 1996;70:861423.PubMed

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DOI: 10.3201/eid1608.091803

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Table of Contents – Volume 16, Number 8—August 2010

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