Volume 16, Number 8—August 2010
Letter
Mycobacterium avium subsp. hominissuis Infection in Horses
Petr Kriz, Petr Jahn, Barbora Bezdekova, Mariana Blahutkova, Vojtech Mrlik, Iva Slana, and Ivo Pavlik
Table
Detection of Mycobacterium avium subsp. hominissuis in tissues of a 1-year-old Fjord filly*
| Sample source | Mycobacteria detection |
qPCR |
|||
|---|---|---|---|---|---|
| Microscopy | Culture | IS1245† | IS901 | ||
| Feces | − | + | 6.31 × 105 | − | |
| Lymph node of transversal colon | + | + | 1.84 × 109 | − | |
| Lymph node of descending colon | + | + | 5.89 × 109 | − | |
| Transversal colon wall | + | + | 3.98 × 107 | − | |
| Descending colon wall | + | + | 6.33 × 106 | − | |
| Liver | − | + | NT | NT | |
| Mesenteric lymph node | +++ | + | 2.47 × 1011 | − | |
| Kidney | − | + | NT | NT | |
| Spleen | − | + | NT | NT | |
| Diaphragm | − | − | 8.22 × 104 | − | |
*qPCR, quantitative real-time PCR; −, negative finding; +, few acid-fast rods (AFR); NT, not tested; +++, >100 AFR (per 50 microscopic fields).
†No. IS1245 copies/g.
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