Recurrent Granulibacter bethesdensis Infections and Chronic Granulomatous Disease
David E. Greenberg
, Adam R. Shoffner, Adrian M. Zelazny, Michael E. Fenster, Kol A. Zarember, Frida Stock, Li Ding, Kimberly R. Marshall-Batty, Richard L. Wasserman, David F. Welch, Kishore Kanakabandi, Dan E. Sturdevant, Kimmo Virtaneva, Stephen F. Porcella, Patrick R. Murray, Harry L. Malech, and Steven M. Holland
Author affiliations: Author affiliations: National Institutes of Health, Bethesda, Maryland, USA (D.E. Greenberg, A.R. Shoffner, A.M. Zelazny, M.E. Fenster, K.A. Zarember, F. Stock, L. Ding, K.R. Marshall-Batty, P.R. Murray, H.L. Malech, S.M. Holland); University of Texas Southwestern Medical Center, Dallas, Texas, USA (R.L. Wasserman, D.F. Welch); Children's Medical Center, Dallas (D.F. Welch); Medical City Dallas Hospital, Dallas (D.F. Welch); National Institutes of Health, Hamilton, Montana, USA (K. Kanakabandi, D.E. Sturdevant, K. Virtaneva, S.F. Porcella)
Figure 3. Detection of Granulibacter sp. DNA by PCR of the spleen of a patient with chronic granulomatous disease. Lanes 1 and 2, no template controls for each primer set; lane 3, 400 ng of spleen DNA amplifying Granulibacter sp. 16S rRNA gene; lane 4, 400 ng of spleen DNA amplifying the Granulibacter sp. methanol dehydrogenase gene; lane 5, 100 ng DNA from the G. bethesdensis type strain amplifying the 16S rRNA gene (positive control); lane 6, 100 ng DNA from the G. bethesdensis type strain amplifying the methanol dehydrogenase gene (positive control). Left lane, molecular mass ladder. Expected PCR product sizes were 137 bp for the 16S rRNA gene and 63 bp for the methanol dehydrogenase gene. Values on the left are in bp.
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