Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 17, Number 2—February 2011

Dengue Virus Serotype 3 Subtype III, Zhejiang Province, China

On This Page
Article Metrics
citations of this article
EID Journal Metrics on Scopus

Cite This Article

To the Editor: Beginning in July 2009, physicians in the city of Yiwu, Zhejiang Province, People’s Republic of China, noted an outbreak of illness characterized by rash, headache, subjective fever, itching, anorexia, and arthritis. We present the results of the investigation of this outbreak, which was caused by dengue virus (DENV) serotype 3 (DENV-3) subtype III.

DENV-3 subtype III has been continuously circulating in the Indian subcontinent since the 1960s. The virus was first isolated from East Africa in 1985 in Mozambique and subsequently in Kenya (1991) and Somalia (1993) (1,2). Although dengue has occurred frequently in southern China, including Guangdong, Guangxi, Hainan, Fujian, and Zhejiang Provinces and in Taiwan (36), to our knowledge, DENV-3 subtype III has not been reported in China.

Yiwu is in the center of Zhejiang Province, southeastern China. This investigation included the entire town of Yiwu and towns that are part of the larger town of Yiting where the outbreak took place. We reviewed medical records and conducted prospective surveillance at all hospitals, health centers, and outpatient clinics in Yiwu to identify patients with suspected dengue fever (DF) during July 1 through October 31, 2009. According to the diagnostic criteria for DF (WS216–2008) enacted by the Chinese Ministry of Health, a patient with suspected disease had at least 2 of the following symptoms: acute onset of rash, headache, subjective fever, itching, anorexia, or arthralgia. Patients with suspected disease were asked to provide blood specimens during the acute phase (within 7 days after symptom onset).

Serum samples were tested by ELISA for immunoglobulin (Ig) M against DENV by using the E-DEN01M kit (Panbio, Sinnamon Park, Queensland, Australia). Acute-phase serum samples were tested by real-time PCR for DENV RNA, according to the diagnostic criteria for dengue fever enacted by the Chinese Ministry of Health. Samples that were positive for DENV-3 by real-time PCR were inoculated into Aedes albopictus mosquito clone C6/36. Primers for reverse transcription–PCR and sequencing of the envelope gene of DENV isolates were used to identify DENV (4).

We considered a patient to have a confirmed case if DENV RNA was detected in the serum by real-time PCR or if IgM against DENV was present. A patient was considered to have a clinically diagnosed case if he or she had acute onset of rash, headache, subjective fever, itching, anorexia, and leukopenia, and lived in Qingsu, Fantianzhu, Xitian, or Shangzhai (4 adjoining villages in the area of the first confirmed case).

The sequences of isolates from case-patients were compared with published sequences by using the BLAST program (, and phylogenic analysis was calculated with PAUP 4.0 β 10 (7), which ran an unrooted tree with 1,000 bootstrap replicates.

We identified 196 cases of DF; 279 suspected cases were excluded, and no cases of dengue hemorrhagic fever or dengue shock syndrome were found. Of DF cases, 71 (36.2%) were confirmed and 125 (63.8%) were clinically diagnosed. Acute-phase serum samples were collected within 7 days after the onset of illness from 350 patients with suspected DF, and dengue virus RNA was detected in samples from 65 patients (18.6%). Six samples had IgM against DENV.


Thumbnail of Evolutionary analysis of dengue virus isolates from this study (boldface) compared with established dengue virus serotype 3 subtypes, Zhejiang Province, People’s Republic of China, 2009.

Figure. Evolutionary analysis of dengue virus isolates from this study (boldface) compared with established dengue virus serotype 3 subtypes, Zhejiang Province, People’s Republic of China, 2009.

Twenty-six samples positive for DENV RNA by real-time PCR were randomly selected to isolate viruses; 23 isolates were cultured. All isolates were amplified by reverse transcription–PCR, and amplicons were sequenced. The envelope gene sequences of all isolates were identical and 1,479 nt in length. All sequences had 99% similarity to 1 DENV serotype 3 partial envelope gene (GenBank accession no. AM746229), which had been detected in Jeddah, Saudi Arabia, in 2004. According to evolutionary analysis (Figure), sequences of our study were also most closely related to the isolate from Saudi Arabia, which suggests that the outbreak may have been initiated by imported cases from the Indian subcontinent or western Asia.

The date of symptom onset among patients with confirmed or clinically diagnosed cases ranged from July 20 to October 4, 2009. Cases peaked in early September and subsided in early October. The median age of patients with confirmed or probable disease was 47.3 years (range 3–96 years). Infections occurred in all age groups, but most infections occurred among persons 41 to 65 years of age; 118 (60.2%) were women, and 172 were farm workers.

Confirmed and clinically diagnosed cases occurred in 18 villages, which were part of 7 towns. Most cases (182) were reported in Yiting, where the first case was confirmed, and in particular, were in persons who lived in the villages of Qingsu, 100 cases; Fantianzhu, 49 cases; Xitian, 19 cases; Shangzhai, 4 cases; and Xiateng, 4 cases.

The outbreak shows that DENV-3 subtype III is easily transmitted among humans and mosquitoes and can adapt efficiently to a new area. Other countries where the climate is similar to that of Zhejiang Province (subtropical monsoon) should be aware of the risk for expansion of DENV-3 subtype III transmission. Clinical vigilance and strong epidemiologic and laboratory surveillance are essential.



We thank the physicians and staff at Health Bureau of Zhejiang Province, the Yiwu Community Health Centers, the Yiwu Department of Health Service, and Yiwu Center for Disease Control and Prevention for their support and assistance with this investigation.


Jimin SunComments to Author , Junfen Lin, Juying Yan, Weizhong Fan, Liang Lu, Huakun Lv, Juan Hou, Feng Ling, Tao Fu, Zhiping Chen, Liming Cong, Qiyong Liu, Yanjun Zhang, and Chengliang Chai
Author affiliations: Author affiliations: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, People’s Republic of China (J. Sun, J. Lin, J. Yan, J. Hou, F. Ling, Z. Chen, L. Cong, Y. Zhang, C. Chai); Yiwu Municipal Center for Disease Control and Prevention , Yiwu, People’s Republic of China (W. Fan, T. Fu); State Key Laboratory for Infectious Disease Prevention and Control, Beijing, People’s Republic of China (Q. Liu)



  1. Gubler  DJ, Sather  GE, Kuno  G, Cabral  JR. Dengue 3 virus transmission in Africa. Am J Trop Med Hyg. 1986;35:12804.PubMedGoogle Scholar
  2. Kanesa-thasan  N, Chang  GJ, Smoak  BL, Magill  A, Burrous  MJ, Hoke  CHJ. Molecular and epidemiologic analysis of dengue virus isolates from Somalia. Emerg Infect Dis. 1998;4:299303. DOIPubMedGoogle Scholar
  3. Qiu  FX, Gubler  DJ, Liu  JC, Chen  QQ. Dengue in China: a clinical review. Bull World Health Organ. 1993;71:34959.PubMedGoogle Scholar
  4. Juying  Y, Yiyu  L, Jingqing  W, Haiyan  M, Yan  F, Wen  SH, The etiological study of a dengue fever outbreak and the molecular characterization of the dengue virus isolates in Zhejiang Province. Chin J Virol. 2006;22:33944.
  5. Lei  L. Comparison of epidemiological characteristics of dengue fever between 2002 and 2006, Guangzhou. S China J Prev Med. 2008;34:1821.
  6. Shaojian  C, Rongtao  H, Nengxiong  Z, Jianming  O, Yansheng  Y. Epidemiological analysis of dengue fever outbreak in 2004 from Fujian Province. Haixia J Prev Med. 2006;12:324.
  7. Swofford  DL. PAUP*: phylogenetic analysis using parsimony and other methods, Version 4.0b9. Sunderland (MA): Sinauer Associates; 2002.




Cite This Article

DOI: 10.3201/eid1702.100396

1These authors contributed equally to this article.

Related Links


Table of Contents – Volume 17, Number 2—February 2011

EID Search Options
presentation_01 Advanced Article Search – Search articles by author and/or keyword.
presentation_01 Articles by Country Search – Search articles by the topic country.
presentation_01 Article Type Search – Search articles by article type and issue.



Please use the form below to submit correspondence to the authors or contact them at the following address:

Jimin Sun, Zhejiang Centers for Disease Control, Xinching Rd, Binjiang District, Hangzhou City, People’s Republic of China 310051

Send To

10000 character(s) remaining.


Page created: July 08, 2011
Page updated: July 08, 2011
Page reviewed: July 08, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.