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Volume 17, Number 3—March 2011
Research

Novel Picornavirus in Turkey Poults with Hepatitis, California, USA

Kirsi S. Honkavuori, H. L. Shivaprasad, Thomas BrieseComments to Author , Craig Street, David L. Hirschberg, Stephen K. Hutchison, and W. Ian Lipkin
Author affiliations: Author affiliations: Columbia University, New York, New York, USA (K.S. Honkavuori, T. Briese, C. Street, D.L. Hirschberg, W.I. Lipkin); California Animal Health and Food Safety Laboratory System, Tulare, California, USA (H.L. Shivaprasad); 454 Life Sciences, Branford, Connecticut, USA (S.K. Hutchison)

Main Article

Table 1

Real-time PCR measurement of viral sequences in organ samples from turkey poults with turkey viral hepatitis compared with controls, California, USA, 2008–2010*

Poult no. Age, d Organ Ct Virus copies†
Infected
2993A 25 Liver 17.12 5.66 × 107
2993B 25 Liver 17.02 6.22 × 107
2993C 25 Liver 26.98 9.0 × 103
2993D 25 Liver 17.93 2.8 × 107
0091.1 28 Liver 17.24 5.1 × 10
0091.2 28 Liver 17.69 3.43 × 107
0091.3 28 Liver 23.26 2.57 × 105
1813.1 26 Liver 23.48 2.12 × 105
1813.2 26 Liver 21.23 1.53 × 106
1813.3 26 Intestine 20.94 1.97 × 106
0690 30 Liver 34.92 8.98 × 10°
1999 29 Liver 28.44 8.42 × 103
Pancreas 25.97 7.32 × 104
Intestine
 24.85
 1.96 × 105
Control
1621.1 42 Liver >36‡ Negative
1621.2 42 Liver >36‡ Negative
1621.3 42 Liver >36‡ Negative

*Ct, cycle threshold.
†In 300 ng total RNA. Copy numbers were calculated on the basis of a standard curve generated from cloned target sequences.
‡Ct >36 was rated as negative on the basis of the highest dilution of standard representing 5 copies.

Main Article

Page created: July 25, 2011
Page updated: July 25, 2011
Page reviewed: July 25, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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