Volume 17, Number 4—April 2011
Dispatch
Molecular Discrimination of Sheep Bovine Spongiform Encephalopathy from Scrapie
Laura Pirisinu, Sergio Migliore, Michele Angelo Di Bari, Elena Esposito, Thierry Baron, Claudia D’Agostino, Luigi De Grossi, Gabriele Vaccari, Umberto Agrimi, and Romolo Nonno
Table 1
Transmissible spongiform encephalopathy isolates analyzed by conformational stability and solubility assay*
| Source | Identification no. | PrP genotype† | GdnHCl1/2, mol/L ± SD‡ |
|---|---|---|---|
| Natural isolates | |||
| Scrapie | ES/8/10/2 | ARQ/ARQ | 2.19 ± 0.18 |
| CH1641-like | 99–454 | VRQ/VRQ | 2.00 ± 0.06 |
| 99–321 | VRQ/VRQ | 2.41 ± 0.49 | |
| TR316211 |
ARQ/ARQ |
2.82 ± 0.08 |
|
| Experimental samples | |||
| CH1641 | 241/74 | AxQ/AxQ | 2.07 ± 0.05 |
| Sheep BSE | 301/16§ | ARQ/ARQ | >4 |
| 301/44§ | ARQ/ARQ | >4 | |
| 302/90¶ | ARQ/ARQ | 3.8; >4; >4 | |
*PrP, prion protein; GdnHCL1/2, guanidine hydrochloride at a concentration able to dissolve half the insoluble aggregates in a brain homogenate; BSE, bovine spongiform encephalopathy.
†Amino acids at codons 136, 154 and 171.
‡Each sample was analyzed >3 times.
§Intracerebral transmission.
¶Oral transmission.
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Page updated: July 25, 2011
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