Volume 17, Number 5—May 2011
Dispatch
Tick-Borne Encephalitis Virus, Kyrgyzstan
Table 2
Primers used to test rodents, insectivores, and ticks for tick-borne encephalitis virus, Kyrgyzstan, 2007 and 2009*
Primer | Gene | Sequence, 5′ → 3′ | Reference |
---|---|---|---|
FSM-1 | NS5 | GAGGCTGAACAACTGCACGA | (8) |
FSM-2 | NS5 | GAACACGTCCATTCCTGATCT | (8) |
FSM-1i | NS5 | ACGGAACGTGACAAGGCTAG | (8) |
FSM-2i | NS5 | GCTTGTTACCATCTTTGGAG | (8) |
TBEV913F | E | TGCACACAYYTGGAAAACAGGGA | (9) |
TBEV1738R | E | TGGCCACTTTTCAGGTGGTACTTGGTTCC | (9) |
RH TBE forward | E | GGCAGCATTGTGACCTGTGT | R. Hewson, unpub. data |
RH TBE reverse | E | CGTGTCCTGTGGCTTTCTTTTT | R. Hewson, unpub. data |
RH TBE probe | E | 6FAM-AGGYGKCYTGTGAGGC-MGB NFQ | R. Hewson, unpub. data |
*TBEV, tick-borne encephalitis virus; NS, nonstructural protein; E, envelope.
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