Volume 17, Number 5—May 2011
Dispatch
Rickettsia parkeri in Gulf Coast Ticks, Southeastern Virginia, USA
Table 1
Sequences of primers and probes used to test for Rickettsia spp. DNA in Amblyomma maculatum ticks collected from southeastern Virginia, April–September 2010*
Name | Sequence, 5′ → 3′ | Gene | Fragment | Reference |
---|---|---|---|---|
Rpa129F | CAAATGTTGCAGTTCCTCTAAATG | ompB | 96 | J. Jiang et al., unpub. data |
Rpa224R | AAAACAAACCGTTAAAACTACCG | ompB | 96 | J. Jiang et al., unpub. data |
Rpa188Probe |
6-FAM-CGCGAAATTAATACCCTTATGAGCAGCAGTCGCG-BHQ-1 |
ompB |
96 |
J. Jiang et al., unpub. data |
R17K128F2 | GGGCGGTATGAAYAAACAAG | 17-kDa antigen gene | 111 | J. Jiang et al., unpub. data |
R17K238R | CCTACACCTACTCCVACAAG | 17-kDa antigen gene | 111 | J. Jiang et al., unpub. data |
R17K202TaqP |
FAM-CCGAATTGAGAACCAAGTAATGC-TAMRA |
17-kDa antigen gene |
111 |
J. Jiang et al., unpub. data |
190-FN1 | AAGCAATACAACAAGGTC | ompA | 540 | (1) |
190-RN1 |
TGACAGTTATTATACCTC |
ompA |
540 |
(1) |
RompB11F | ACCATAGTAGCMAGTTTTGCAG | ompB | 1895 | (9) |
RompB1902R | CCGTCATTTCCAATAACTAACTC | ompB | 1895 | (9) |
*omp, outer membrane protein gene.
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